Tartrate-resistant acid phosphatase (TRAP) and the osteoclast/immune cell dichotomy

Hayman, Alison R.

doi:10.1080/08916930701694667

Cited by 310 publications

(254 citation statements)

References 48 publications

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“…In our culture system, IL-3 inhibited RANKL-induced osteoclast differentiation by directly acting on osteoclast precursors. Tartrate-resistant acid phosphatase is a marker of osteoclast but is not specific for human osteoclasts (35). Therefore, we determined the expression of 23c6, an epitope of the vitronectin receptor (CD51/61), which is specifically expressed by human osteoclasts (36).…”

Section: Discussionmentioning

confidence: 99%

IL-3 Inhibits Human Osteoclastogenesis and Bone Resorption through Downregulation of c-Fms and Diverts the Cells to Dendritic Cell Lineage

Gupta

Barhanpurkar

Tomar

et al. 2010

The Journal of Immunology

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IL-3 is an important cytokine that regulates hematopoiesis and functions as a link between the immune and the hematopoietic system. In this study, we investigated the role and mechanism of IL-3 action on human osteoclast formation and bone resorption using PBMCs. PBMCs differentiate into functional osteoclasts in the presence of M-CSF and receptor activator of NF-κB ligand as evaluated by 23c6 expression and bone resorption. We found that IL-3 dose-dependently inhibited formation of 23c6-positive osteoclasts, bone resorption and C-terminal telopeptide of type I collagen, a collagen degradation product. The inhibitory effect of IL-3 on bone resorption was irreversible. To investigate the mechanism of IL-3 action, we analyzed the effect of IL-3 on the receptor activator of NF-κB and c-Fms receptors and c-Fos, PU.1, NFAT cytoplasmic 1, and RelB transcription factors essential for osteoclastogenesis. IL-3 significantly inhibited c-Fms and downregulated both PU.1 and c-Fos at both mRNA and protein level. Furthermore, IL-3–treated cells showed increased expression of dendritic cell markers CD1a and CD80 and decreased expression of monocyte/macrophage marker CD14. Interestingly, IL-3 inhibited formation of human osteoclasts derived from blood monocytes and bone marrow cells of osteoporotic individuals. Thus, IL-3 may have therapeutic potential as an antiosteolytic agent in treatment of osteoporosis.

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Section: Discussionmentioning

confidence: 99%

IL-3 Inhibits Human Osteoclastogenesis and Bone Resorption through Downregulation of c-Fms and Diverts the Cells to Dendritic Cell Lineage

Gupta

Barhanpurkar

Tomar

et al. 2010

The Journal of Immunology

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“…16 TRAP staining (TRAP 387 Kit, Sigma-Aldrich Chemicals, St Louis, Mo), a marker of osteoclasts, was used to evaluate alveolar bone resorption. 7,12,13,[17][18][19] The osteoclasts were multinucleated, TRAP-positive cells in contact with or near bone surfaces 12,13 and were evaluated in the periodontal ligament (PL) of the mesial face of the DV root of the maxillary right first molar. 18,20,21 From each of the five sections, images were captured from five consecutive microscopic fields 18 using an Olympus BX40 light microscope (Olympus, Tokyo, Japan) coupled to a Dinolite AM 423X microcamera (AmMo Electronics Corp, New Taipei City, Taiwan) at 4003 magnification.…”

Section: Tissue Responses To Corticotomy-and Corticision-assisted Toomentioning

confidence: 99%

Tissue responses resulting from tooth movement surgically assisted by corticotomy and corticision in rats

Peron

Johann

Papalexiou

et al. 2016

The Angle Orthodontist

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Objective: To compare the histological responses in corticotomy-and corticision-assisted tooth movement. Materials and Methods: Ninety Wistar rats were divided into three groups: C (control-tooth movement only), CT (tooth movement þ corticotomy), and CI (tooth movement þ corticision). Surgeries were performed on the vestibular and lingual cortical bone of the maxillary first molar. Tooth movement was carried out with nickel-titanium closed coil springs having a force of 30 g. The rats were sacrificed at 3, 14, and 28 days. To evaluate the number of osteoclasts and amount of root resorption, a tartrate-resistant acid phosphatase stain was used. Hematoxylin and eosin staining was performed for areas of hyalinization, and the organic bone matrix was stained with picrosirius. Results: The CT group showed a greater number of osteoclasts than did the C group on day 3 (P , .05). At the same time point, the CT and CI groups showed a delayed onset of organic bone matrix remodeling and a lower incidence of root resorption than did the C group (P , .05). There were also fewer hyalinization areas in the CI group than in the C group on day 3 (P , .05). Conclusions: Corticotomy effectively increased bone resorption during the early stages of tooth movement, but this increase was not observed for corticision. The surgical procedures did not accelerate organic bone matrix remodeling. Corticotomies and corticisions decreased the risk of root resorption only during the early stages of movement. Corticision reduced the level of hyalinization, while corticotomy did not. (Angle Orthod. 2017;87:118-124)

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“…27 TRAP staining was carried out using a manufacturer's protocol (387A-1KT; Sigma Aldrich, Inc., St. Louis, MO). The number of TRAP-positive cells was determined within an area of 8x6mm around the center of the defect (Figs.…”

Section: Tartrate-resistant Acid Phosphatasementioning

confidence: 99%

Bone Marrow-Derived Cell Concentrates Have Limited Effects on Osteochondral Reconstructions in the Mini Pig

Jagodzinski

Liu

Guenther

et al. 2014

Tissue Engineering Part C: Methods

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This study investigates the effects of seeding a chondrogenic and osteogenic scaffold with a bone marrowderived cell concentrate (BMCC) and reports the histological and mechanical properties 3 months after implantation in the miniature pig. Twenty defects (7 · 10 mm) were created in the femoral condyles of 10 miniature pigs. The defects were left empty (E), filled with the grafted cylinder upside down (U) or with a combined scaffold (S) containing a spongious bone cylinder (Tutobone Ò ) covered with a collagen membrane (Chondrogide Ò ). In a fourth group, the same scaffolds were implanted but seeded with a stem cell concentrate (S + BMCC). The animals were stained with calcein green after 2 weeks and xylenol orange after 4 weeks. After 3 months, the animals were sacrificed, and a mechanical analysis (Young's modulus), macroscopic, and histologic (ICRS Score) examination of the specimens was conducted. Young's modulus in the periphery was significantly lower for group E (67.5 -15.3 kPa) compared with untreated controls (171.7 -21.6 kPa, p < 0.04). Bone defects were smaller in group S (10% -8%) compared with E (27% -7%; p < 0.05). There was a trend toward smaller bony defects on comparing groups E and S + BMCC (11% -8%; p = 0.07). More red fluorescence was detected in group S + BMCC (2.3% -1.1%) compared with groups E (0.4% -0.2%) and U (0.5% -0.2%, p < 0.03). ICRS scores were higher for groups S (25.3 -3.8) and S + BMCC (26.2 -5.2, p < 0.01). In this animal model of osteochondral defects, stem cell concentrates enhance new bone apposition but fail to improve mechanical properties or histological appearance of cartilage regenerates in critical-sized defects.

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Tartrate-resistant acid phosphatase (TRAP) and the osteoclast/immune cell dichotomy

Cited by 310 publications

References 48 publications

IL-3 Inhibits Human Osteoclastogenesis and Bone Resorption through Downregulation of c-Fms and Diverts the Cells to Dendritic Cell Lineage

IL-3 Inhibits Human Osteoclastogenesis and Bone Resorption through Downregulation of c-Fms and Diverts the Cells to Dendritic Cell Lineage

Tissue responses resulting from tooth movement surgically assisted by corticotomy and corticision in rats

Bone Marrow-Derived Cell Concentrates Have Limited Effects on Osteochondral Reconstructions in the Mini Pig

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