TAT-conjugated nanodiamond for the enhanced delivery of doxorubicin

Abstract

The unique properties of nanodiamonds (NDs) such as chemical stability, surface modifiability and remarkable biocompatibility impart them with a great opportunity to be versatile platforms for drug delivery. In this study, chemotherapeutic doxorubicin (DOX) and cell penetrating peptide TAT were conjugated to the surface of NDs in sequence through carbodiimide coupling in order to avoid premature release and enhance the intracellular delivery of DOX. The cytotoxicity, intracellular location and cellular uptake of DOX-conjugated NDs with or without TAT were evaluated in C6 glioma cells. Our results revealed that conjugation of TAT to ND-DOX could enhance the translocation across the cell membrane and exhibit a higher cytotoxicity effect than free DOX. This antitumor drug and penetrating peptide-conjugated ND drug delivery system therefore represents a novel delivery system with promoted antineoplastic activity of therapeutics and minimized side effects.

Nanodiamonds coupled with plant bioactive metabolites: A nanotech approach for cancer therapy

“…In this context, doxorubicin, one of the most common anticancer drugs, was successfully linked to nanodiamonds and introduced toward murine macrophages, as well as human colorectal carcinoma cells, preserving its efficacy [19,20]. Nanodiamond-mediated doxorubicin delivery system was also able to inhibit the generation of lung metastasis in mice, starting from 4T1 breast cancer cells [21].…”

Nanodiamonds coupled with 5,7-dimethoxycoumarin, a plant bioactive metabolite, interfere with the mitotic process in B16F10 cells altering the actin organization

“…[5][6][7] In particular, the successful use of DNDs in cancer treatment has greatly captured the attention of the scientific community. [8][9][10][11] Although numerous plant compounds have been widely studied for their antitumor activities, [12][13][14][15] to date only three papers describe the coupling of natural secondary metabolites with DNDs aimed at enhancing their bioavailability and bioactivity. The first of them illustrated the antimicrobial activity of menthol ((1R,2S,5R)-2-isopropyl-5-methylcyclohexanol)-modified ND particles.…”

Nanodiamonds as Intracellular Probes for Imaging in Biology and Medicine

“…12) ) ND conjugates with cisplatin and 10-hydroxycamptothecin (HCPT) show a similar particle architecture Guan et al 2010). Conjugation of DOX-ND with cell-penetrating TAT peptide (HIV trans-activator of transcription protein) enhances intracellular delivery and blocks premature release (Li et al 2011a). DOX can be bound to FNDs which, unlike DNDs, allow long-term fluorescence localization of particles within the cell.…”