Taxon K, a complex within the Burkholderia cepacia complex, comprises at least two novel species, Burkholderia contaminans sp. nov. and Burkholderia lata sp. nov.

Vanlaere, Elke; Baldwin, Amy; Gevers, Dirk; Henry, Deborah A.; Brandt, Evie De; LiPuma, John J.; Mahenthiralingam, Eshwar; Speert, David P.; Dowson, Christopher G.; Vandamme, Peter

doi:10.1099/ijs.0.001123-0

Cited by 271 publications

(194 citation statements)

References 50 publications

Supporting

Mentioning

187

Contrasting

Unclassified

Order By: Relevance

“…[69][70][71][72][73][74][75][76][77][78][79][80] The frequency of detection of these species in people with CF varies considerably, although Burkholderia cenocepacia and Burkholderia multivorans are most common. 26,81 Burkholderia gladioli is the third most frequently isolated Burkholderia species among CF patients in the United States.…”

Section: Ic2 Burkholderia Sppmentioning

confidence: 99%

Infection Prevention and Control Guideline for Cystic Fibrosis: 2013 Update

Saiman

Siegel

LiPuma

et al. 2014

Infect. Control Hosp. Epidemiol.

352

382

View full text Add to dashboard Cite

The 2013 Infection Prevention and Control (IP&C) Guideline for Cystic Fibrosis (CF) was commissioned by the CF Foundation as an update of the 2003 Infection Control Guideline for CF. During the past decade, new knowledge and new challenges provided the following rationale to develop updated IP&C strategies for this unique population:1. The need to integrate relevant recommendations from evidence-based guidelines published since 2003 into IP&C practices for CF. These included guidelines from the Centers for Disease Control and Prevention (CDC)/Healthcare Infection Control Practices Advisory Committee (HICPAC), the World Health Organization (WHO), and key professional societies, including the Infectious Diseases Society of America (IDSA) and the Society for Healthcare Epidemiology of America (SHEA). During the past decade, new evidence has led to a renewed emphasis on source containment of potential pathogens and the role played by the contaminated healthcare environment in the transmission of infectious agents. Furthermore, an increased understanding of the importance of the application of implementation science, monitoring adherence, and feedback principles has been shown to increase the effectiveness of IP&C guideline recommendations.2. Experience with emerging pathogens in the non-CF population has expanded our understanding of droplet transmission of respiratory pathogens and can inform IP&C strategies for CF. These pathogens include severe acute respiratory syndrome coronavirus and the 2009 influenza A H1N1. Lessons learned about preventing transmission of methicillin-resistant Staphylococcus aureus (MRSA) and multidrug-resistant gram-negative pathogens in non-CF patient populations also can inform IP&C strategies for CF.

show abstract

Section: Ic2 Burkholderia Sppmentioning

confidence: 99%

Infection Prevention and Control Guideline for Cystic Fibrosis: 2013 Update

Saiman

Siegel

LiPuma

et al. 2014

Infect. Control Hosp. Epidemiol.

352

382

View full text Add to dashboard Cite

show abstract

“…Many Burkholderia have developed beneficial interactions with their plant hosts and have considerable ecological significance: several species of Burkholderia have proven to be very efficient biocontrol and bioremediation agents (Chiarini et al, 2006). This is especially true for members of the Burkholderia cepacia complex (Bcc), which currently comprises 17 closely related genomovars (Vanlaere et al, 2009). For instance, Burkholderia ambifaria J82 was registered by the US Environmental Protection Agency for use as a biospecticide and was commercially used for a few years (Parke and GurianSherman, 2001).…”

Section: Introductionmentioning

confidence: 99%

Phase variation has a role in Burkholderia ambifaria niche adaptation

et al. 2009

View full text Add to dashboard Cite

Members of the Burkholderia cepacia complex (Bcc), such as B. ambifaria, are effective biocontrol strains, for instance, as plant growth-promoting bacteria; however, Bcc isolates can also cause severe respiratory infections in people suffering from cystic fibrosis (CF). No distinction is known between isolates from environmental and human origins, suggesting that the natural environment is a potential source of infectious Bcc species. While investigating the presence and role of phase variation in B. ambifaria HSJ1, an isolate recovered from a CF patient, we identified stable variants that arose spontaneously irrespective of the culture conditions. Phenotypic and proteomic approaches revealed that the transition from wild-type to variant types affects the expression of several putative virulence factors. By using four different infection models (Drosophila melanogaster, Galleria mellonella, macrophages and Dictyostelium discoideum), we showed that the wild-type was more virulent than the variant. It may be noted that the variant showed reduced replication in a human monocyte cell line when compared with the wild-type. On the other hand, the variant of isolate HSJ1 was more competitive in colonizing plant roots than the wild-type. Furthermore, we observed that only clinical B. ambifaria isolates generated phase variants, and that these variants showed the same phenotypes as observed with the HSJ1 variant. Finally, we determined that environmental B. ambifaria isolates showed traits that were characteristic of variants derived from clinical isolates. Our study therefore suggest that B. ambifaria uses phase variation to adapt to drastically different environments: the lung of patients with CF or the rhizosphere.

show abstract

“…The Burkholderia cepacia complex comprises closely related species of Gram-negative bacteria commonly found in the environment (1,2). B. cepacia complex bacteria are opportunistic human pathogens that establish chronic lung infections in immunocompromised patients with chronic granulomatous disease or, most commonly, cystic fibrosis (3).…”

mentioning

confidence: 99%

BcsKC Is an Essential Protein for the Type VI Secretion System Activity in Burkholderia cenocepacia That Forms an Outer Membrane Complex with BcsLB

Aubert

MacDonald

Valvano

2010

Journal of Biological Chemistry

View full text Add to dashboard Cite

The type VI secretion system (T6SS) contributes to the virulence of Burkholderia cenocepacia, an opportunistic pathogen causing serious chronic infections in patients with cystic fibrosis. BcsK C is a highly conserved protein among the T6SSs in Gram-negative bacteria. Here, we show that BcsK C is required for Hcp secretion and cytoskeletal redistribution in macrophages upon bacterial infection. These two phenotypes are associated with a functional T6SS in B. cenocepacia. Experiments employing a bacterial two-hybrid system and pulldown assays demonstrated that BcsK C interacts with BcsL B , another conserved T6SS component. Internal deletions within BcsK C revealed that its N-terminal domain is necessary and sufficient for interaction with BcsL B . Fractionation experiments showed that BcsK C can be in the cytosol or tightly associated with the outer membrane and that BcsK C and BcsL B form a high molecular weight complex anchored to the outer membrane that requires BcsF H (a ClpV homolog) to be assembled. Together, our data show that BcsK C /BcsL B interaction is essential for the T6SS activity in B. cenocepacia.

show abstract

Taxon K, a complex within the Burkholderia cepacia complex, comprises at least two novel species, Burkholderia contaminans sp. nov. and Burkholderia lata sp. nov.

Cited by 271 publications

References 50 publications

Infection Prevention and Control Guideline for Cystic Fibrosis: 2013 Update

Infection Prevention and Control Guideline for Cystic Fibrosis: 2013 Update

Phase variation has a role in Burkholderia ambifaria niche adaptation

BcsKC Is an Essential Protein for the Type VI Secretion System Activity in Burkholderia cenocepacia That Forms an Outer Membrane Complex with BcsLB

Contact Info

Product

Resources

About