Taxonomic considerations among and within some Egyptian taxa of <i>Capparis</i> and related genera (Capparaceae) as revealed by RAPD fingerprinting

Abstract

Taxonomic considerations among and within some Egyptian taxa of Capparis and related genera (Capparaceae) as revealed by RAPD fingerprinting.-This investigation was carried out to assess the taxonomic relationships among eight taxa of the Egyptian members of Capparaceae based on random amplified polymorphic DNA markers, and to compare the results with those obtained from morphological studies. A total of 46 bands were scored for three RAPD primers corresponding to an average of 15.3 bands per primer. The three primers (A03, A07 and A09) revealed eight polymorphic RAPD markers among the studied taxa ranging in size from 200 bp to 1000 bp. Jaccard's coefficient of similarity varied from 0.28 to 0.84, indicative of high level of genetic variation among the genotypes studied. uPGMA cluster analysis indicated three distinct clusters, one comprised Cleome amblyocarpa and Gynandropsis gynandra, while another included two clusters at 0.74 phenon line; one for Capparis decidua, and the other for Capparis sinaica and all varieties of Capparis spinosa. The four varieties of Capparis spinosa were segregated at 0.84 phenon line. However, one of these varieties was more closely related to Capparis sinaica than to the other three varieties of C. spinosa. The RAPD analysis reported here confirms previous studies based on morphological markers.Key words: Capparis; Cleome; cluster analysis; Egypt; genetic relationships; Gynandropsis; RAPD-PCR; taxonomy; uPGMA. ResumenConsideraciones taxonómicas sobre algunos taxones egipcios de Capparis y géneros relacionados (Capparaceae) a partir de RAPDs.-El objetivo de este trabajo es investigar las relaciones taxonómicas entre ocho taxones pertenecientes a las Capparaceae en base a marcadores de tipo RAPD, y comparar los resultados con los obtenidos previamente en estudios morfológicos. Se han contabilizado un total de 46 bandas para tres pares de cebadores, con una media de 15,3 bandas por cebador. los tres pares de cebadores (A03, A07 y A09) revelan ocho marcadores polimórficos entre los taxones estudiados, de entre 200 y 1000 pares de bases. El coeficiente de similaridad de Jaccard varía entre 0,28 y 0,84, indicativo de un alto nivel de variación genética entre los genotipos estudiados. El análisis uPGMA muestra tres grupos distintos, el primero comprende Cleome amblyocarpa y Gynandropsis gynandra, mientras que el segundo incluye dos grupos a la altura del valor 0,74 del dendrograma: uno se corresponde con Capparis decidua, y el otro comprende Capparis sinaica y todas las variedades de Capparis spinosa. las cuatro variedades de C. spinosa se segregan a la altura del valor 0,84 del dendrograma. Sin embargo, una de estas variedades está más relacionada con C. sinaica que con las otras variedades de C. spinosa. El análisis de RAPD confirma los resultados de estudios anteriores basados en caracteres morfológicos.

Using genetic structure data and phylogenetic criteria in attributing prioritization scores for conservation of spontaneous Capparis spinosa L. populations from Tunisia

“…The levels of genetic diversity reported here rank within the range of values reported for caper species using DNA marker tools (Gristina et al, 2014;Inocencio, Cowan, Alcaraz, Rivera, & Fay, 2005;Khouildi et al, 2000;Moubasher et al, 2011;Nosrati et al, 2012;Özbek & Kara, 2013;Saifi et al, 2011) and other species with mixed breeding systems (Hamrick & Godt, 1996).…”

A systematic revision of Capparaceae and Cleomaceae in Egypt: an evaluation of the generic delimitations of Capparis and Cleome using ecological and genetic diversity

“…Various traditional taxonomic positions of Capparis and Cleome were derived from the study of both quantitative and qualitative macro-morphological characters which might become a broad margin of mistake. Therefore, additional knowledge about the genotype of plants is much required to resolve taxonomic problems in these genera [54]. Hence, evaluations of molecular statistics has had a serious effect on our perception of plant evolution and relations on all taxonomic levels, from the deep nodes dividing the key plant groups to species and populations [20].…”

A taxonomic revision of the Capparis spinosa group (Capparaceae) from the Mediterranean to Central Asia

“…This hypothesis was recently strengthened by the results of an investigation on Egyptian populations of Capparis based on RAPD fingerprinting (Moubasher et al 2011), showing the affinity of representatives of this subspecies with the Sudano-Zambezian C. spinosa subsp. cartilaginea (Decne.)…”