TCRB Clonotypes Are Present in CD4 T Cell Populations Prepared Directly from Rheumatoid Synovium

Davey, Michael P.; Burgoine, Gary A.; Woody, Carolyn N.

doi:10.1016/s0198-8859(97)00087-6

Cited by 14 publications

(16 citation statements)

References 56 publications

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“…[19][20][21][22][23] Analysis of CDR3 of overexpressed BV genes has revealed some clonotypes that only exist in rheumatoid synovium but not peripheral T cells, suggesting T-cell clonal expansion in the affected joints in RA. [24][25][26] However, clonality and TCR BV gene usage of infiltrating T cells in RA SF or membranes are relatively heterogeneous, which complicates BV gene analysis using regular or semiquantitative PCR. This may be attributable to significant variations in the detection of overexpressed BV genes and CDR3 structural features of RA synovial T cells reported in different studies.…”

Section: Introductionmentioning

confidence: 99%

Skewed T-cell receptor BV14 and BV16 expression and shared CDR3 sequence and common sequence motifs in synovial T cells of rheumatoid arthritis

Sun

Nie²,

et al. 2005

Genes Immun

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T-lymphocytes play an important role in rheumatoid arthritis (RA). In this study, we evaluated the hypothesis that common T-cell receptor (TCR) structural features may exist among infiltrating T cells of different RA patients, if the TCR repertoire is shaped by interaction with common self or microbial antigens in the context of susceptible HLA genes in RA. Synovial lesion tissue (ST), synovial fluid (SF) and blood specimens from RA patients and controls were analyzed for TCR V gene repertoire by real-time PCR. There was highly skewed BV14 and BV16 usage in synovial T cells of RA as opposed to those of controls, which was accompanied with a trend for correlation between skewed BV16 and DRB1*0405. Immunoscope analysis of the V-D-J region of ST-derived T cells demonstrated oligoclonal and polyclonal expansion of BV14 þ and BV16 þ T cells. Detailed characterization using specific BV and BJ primers further revealed common clonotypes combining the same BV14/BV16, BJ and CDR3 length. DNA cloning and sequence analysis of the clonotypes confirmed identical CDR3 sequences and common CDR3 sequence motifs among different RA patients. The findings are important in the understanding of BV gene skewing and CDR3 structural characteristics among synovial infiltrating T cells of RA.

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Section: Introductionmentioning

confidence: 99%

Skewed T-cell receptor BV14 and BV16 expression and shared CDR3 sequence and common sequence motifs in synovial T cells of rheumatoid arthritis

Sun

Nie²,

et al. 2005

Genes Immun

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“…4 The 5' primer for reamplification was always TCRVB 2, 3, or 5S1. The 3' primers were selected based on the TCRBJ gene expressed by the clone of interest.…”

Section: Confirmation Of Clones Within Liver Tissuementioning

confidence: 99%

“…Individual plasmids were then sequenced exactly as previously described. 4 Identical sequences obtained from randomly selected plasmids were inferred to represent the presence of the clone in the resident CD4 T-cell population.…”

Section: Confirmation Of Clones Within Liver Tissuementioning

confidence: 99%

“…This was shown by eluting DNA from that section of the denaturing gel that contained a skewed band, amplifying the DNA by PCR, and performing sequence analysis. 4 In this study, rather than first characterizing bands that deviated from a normal distribution on spectratype analysis, we attempted to clone T cells in vitro and then subsequently confirm the presence of each clone in the resident CD4 population by using the spectratype approach. If the same T cell was cloned twice in vitro, this was inferred to mean that the clone had an increased precursor frequency in situ.…”

Section: Spectratype Analysismentioning

confidence: 99%

“…3 These are referred to as clonotypes to reflect that they were identified by molecular approaches rather than by culture and characterization of intact cells. 4 The peptide/MHC ligands responsible for driving these clonal expansions remain unknown.…”

mentioning

confidence: 99%

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Woody³

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Trivalent T Cell Receptor Peptide Vaccine for Treatment of Multiple Sclerosis Targets Predominant V Genes Widely Implicated in Autoimmune Diseases and Allergy

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TCRB Clonotypes Are Present in CD4 T Cell Populations Prepared Directly from Rheumatoid Synovium

Cited by 14 publications

References 56 publications

Skewed T-cell receptor BV14 and BV16 expression and shared CDR3 sequence and common sequence motifs in synovial T cells of rheumatoid arthritis

Skewed T-cell receptor BV14 and BV16 expression and shared CDR3 sequence and common sequence motifs in synovial T cells of rheumatoid arthritis

T-cell clones derived by CD3 stimulation from hepatitis C virus explanted liver tissue are not representative of dominant clones present in vivo

Trivalent T Cell Receptor Peptide Vaccine for Treatment of Multiple Sclerosis Targets Predominant V Genes Widely Implicated in Autoimmune Diseases and Allergy

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