Teburu—Open source 3D printable bioreactor for tissue slices as dynamic three‐dimensional cell culture models

Daneshgar, Assal; Tang, Peter; Remde, Christopher; Lommel, Michael; Moosburner, Simon; Kertzscher, Ulrich; Klein, Oliver; Weinhart, Marie; Pratschke, Johann; Sauer, Igor M.; Hillebrandt, Karl H.

doi:10.1111/aor.13518

Cited by 7 publications

(6 citation statements)

References 24 publications

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“…This may be due to numerous in-house developments and optimizations in the field of fluidic MBR as PDMS is comparatively easy to process and to shape. Occasionally, 3D printed systems are also used [2,3]. Another large group of widely used MBRs, the RWV bioreactor type, is usually based on commercially available systems, e.g., from Synthecon.…”

Section: Resultsmentioning

confidence: 99%

Advanced 3D Cell Culture Techniques in Micro-Bioreactors, Part I: A Systematic Analysis of the Literature Published between 2000 and 2020

2020

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Bioreactors have proven useful for a vast amount of applications. Besides classical large-scale bioreactors and fermenters for prokaryotic and eukaryotic organisms, micro-bioreactors, as specialized bioreactor systems, have become an invaluable tool for mammalian 3D cell cultures. In this systematic review we analyze the literature in the field of eukaryotic 3D cell culture in micro-bioreactors within the last 20 years. For this, we define complexity levels with regard to the cellular 3D microenvironment concerning cell–matrix-contact, cell–cell-contact and the number of different cell types present at the same time. Moreover, we examine the data with regard to the micro-bioreactor design including mode of cell stimulation/nutrient supply and materials used for the micro-bioreactors, the corresponding 3D cell culture techniques and the related cellular microenvironment, the cell types and in vitro models used. As a data source we used the National Library of Medicine and analyzed the studies published from 2000 to 2020.

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Section: Resultsmentioning

confidence: 99%

Advanced 3D Cell Culture Techniques in Micro-Bioreactors, Part I: A Systematic Analysis of the Literature Published between 2000 and 2020

2020

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“…As well as an in vivo-like structure, these tissues also provide the ECM. The models cover decellularized tissue slices [208] and also decellularized whole organs [204] with permission of Springer Nature (C) Bioreactor system of Mahmoudifar and Doran consisting of two separate culture chambers containing osteogenic respectively chondrogenic medium (left). Up to three triplicate PGA disk scaffolds can be mounted between the two chambers (right).…”

Section: Decellularized Tissues In Fluidic Micro-bioreactorsmentioning

confidence: 99%

“…As well as an in vivo-like structure, these tissues also provide the ECM. The models cover decellularized tissue slices [208] and also decellularized whole organs [209]. The system of Daneshgar et al [208] consisted of decellularized human liver slices, which they reseeded with human mesenchymal stromal cells, in a printed, so-called Teburu perfusion bioreactor.…”

Section: Decellularized Tissues In Fluidic Micro-bioreactorsmentioning

confidence: 99%

“…The models cover decellularized tissue slices [208] and also decellularized whole organs [209]. The system of Daneshgar et al [208] consisted of decellularized human liver slices, which they reseeded with human mesenchymal stromal cells, in a printed, so-called Teburu perfusion bioreactor. Song et al [209] used acellular kidney scaffolds of rats, recellularized with human umbilical venous endothelial cells as well as rat neonatal kidney cells.…”

Section: Decellularized Tissues In Fluidic Micro-bioreactorsmentioning

confidence: 99%

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Advanced 3D Cell Culture Techniques in Micro-Bioreactors, Part II: Systems and Applications

et al. 2020

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In this second part of our systematic review on the research area of 3D cell culture in micro-bioreactors we give a detailed description of the published work with regard to the existing micro-bioreactor types and their applications, and highlight important results gathered with the respective systems. As an interesting detail, we found that micro-bioreactors have already been used in SARS-CoV research prior to the SARS-CoV2 pandemic. As our literature research revealed a variety of 3D cell culture configurations in the examined bioreactor systems, we defined in review part one “complexity levels” by means of the corresponding 3D cell culture techniques applied in the systems. The definition of the complexity is thereby based on the knowledge that the spatial distribution of cell-extracellular matrix interactions and the spatial distribution of homologous and heterologous cell–cell contacts play an important role in modulating cell functions. Because at least one of these parameters can be assigned to the 3D cell culture techniques discussed in the present review, we structured the studies according to the complexity levels applied in the MBR systems.

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“…Assal Daneshgar et al of the Humboldt‐Universität zu Berlin, Berlin, Germany reported on their three‐dimensional tissue culture printable bioreactor system (Teburu) that is applicable for different approaches of pathway investigation and targeted tissue repair using human tissue slices as a three‐dimensional cell culture model. They discussed reseeding a 500‐µm thick decellularized human liver slice using human mesenchymal stroma cells.…”

Section: Tissue Engineeringmentioning

confidence: 99%

Artificial Organs 2019: A year in review

Malchesky¹

2020

Artificial Organs

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In this Editor’s Review, articles published in 2019 are organized by category and summarized. These provide a brief reflection of the research and progress in artificial organs intended to advance and better human life while providing insight for continued application of these technologies and methods. Artificial Organs continues in the original mission of its founders “to foster communications in the field of artificial organs on an international level.” Artificial Organs continues to publish developments and clinical applications of artificial organ technologies in this broad and expanding field of organ Replacement, Recovery, and Regeneration from all over the world. Peer‐reviewed Special Issues this year included contributions from the 14th International Conference on Pediatric Mechanical Circulatory Support Systems and Pediatric Cardiopulmonary Perfusion edited by Dr Akif Undar, and the 26th Congress of the International Society for Mechanical Circulatory Support edited by Dr Minoru Ono and Dr Francesco Moscato. Additionally, important editorials highlighted the need for sustainability in hemodialysis, challenges and opportunities in mechanical circulatory support, progress in artificial pancreas development, historical perspectives on ventilators and dialysis, tissue engineering for cardiac support, and regional updates from India and China. Our Pioneer Series continues to highlight the many researchers who created this field of study. This year we debuted a new series entitled “Recent Progress in Artificial Organs” prepared by Vakhtang Tchantchaleishvili and Elizabeth Maynes of Thomas Jefferson University, Philadelphia, PA, USA. This series highlights recent advances and new developments in the field. We take this time also to express our gratitude to our authors for contributing their work to this journal. We offer our very special thanks to our reviewers who give so generously of their time and expertise to review, critique, and especially provide meaningful suggestions to the author’s work. Without our dedicated expert reviewers, the quality expected from such a journal would not be possible. We also express our special thanks to our Publisher, John Wiley & Sons, for their expert attention and support in the production Artificial Organs. We look forward to reporting further advances in the coming years.

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Teburu—Open source 3D printable bioreactor for tissue slices as dynamic three‐dimensional cell culture models

Cited by 7 publications

References 24 publications

Advanced 3D Cell Culture Techniques in Micro-Bioreactors, Part I: A Systematic Analysis of the Literature Published between 2000 and 2020

Advanced 3D Cell Culture Techniques in Micro-Bioreactors, Part I: A Systematic Analysis of the Literature Published between 2000 and 2020

Advanced 3D Cell Culture Techniques in Micro-Bioreactors, Part II: Systems and Applications

Artificial Organs 2019: A year in review

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