“…MUTZ-3 LC closely resemble their native counterparts, both phenotypically and functionally (Kosten et al, 2015b;Masterson et al, 2002;Santegoets et al, 2008). Indeed, in the past we have shown in skin equivalents with integrated Langerhans Cells (SE-LC) that upon allergen exposure MUTZ-3 LC mature and migrate in a CXCL12 dependent manner from the epidermis to the dermis, whereas upon irritant exposure MUTZ-3 LC migrate in a CCL5 dependent manner and undergo an IL-10 dependent phenotypic switch into a macrophage-like cell in the dermis, closely mimicking our observations in excised skin (Kosten et al, 2015b;Ouwehand et al, 2008Ouwehand et al, , 2011b). Recently, we described a full thickness oral gingiva equivalent (GE) consisting of a reconstructed epithelium on a fibroblast populated collagen hydrogel (lamina propria) and shown that the GE secretes negligible amounts of key chemokines involved in LC migration in skin (Kosten et al, 2015a).…”