Technique for Obtaining Infrared Spectra of Microgram Amounts of Compounds Separated by Thin Layer Chromatography.

Chromatography

“…Reuseable plates have been prepared by spraying molten adsorbents onto rigid supports (604). Special equipment for TLC includes: various sample applicators (77,321,496,1345,1760,2057) and application chambers (77,365,1421), narrow developing chambers (309,739,881,1591,1724,2177) and an adsorbent sandwich (195S), self-contained chambers of adsorbent layers in test tubes (126, 2222) or funnels (1382), sorbentcovered microscope slides (92,1390,1860) and glass rods (509), apparatus for starting and stopping development (2054) and for circular (2110) or centrifugal TLC (1058,1703), a cover plate for staining at elevated temperatures (1593), a high-resolution zonal scraper (1908)(1909)(1910), suction apparatus for collecting zones (648, 1785), and elution set-ups (648,1207,1760,2142).…”

Finding a suitable separation condition for TLC/FTIR analysis by using multiple-narrow-band TLC technique

“…This approach uses suitable solvent to dissolve each separated sample out of the TLC plate. 13,14 Aer removing the solvent by vaporization, IR spectrum of the obtained sample is recorded. This approach is tedious and the analysis is under the risk of sample loss and contamination.…”

Analysis of an Alanine/Arginine Mixture by Using TLC/FTIR Technique

“…Since the 1960s, some TLC/IR technique appeared in the literature [5,6]. In such an experiment, suitable solvents were used to transfer each separated analyte out of the TLC plate.…”