Technique to minimize phenolics in walnut <i>in vitro</i> culture initiation

Mir, Javid Iqbal; Ahmed, Nazeer; Itoo, H.; Sheikh, M. A.; Wani, Shabir Hussain; Rashid, Rebwar Saeed M.; Mir, Hidayatullah

doi:10.5958/0974-0112.2017.00057.3

Cited by 6 publications

(4 citation statements)

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“…Ascorbic acid has been used successfully in the past to inhibit the exudation of phenols and reduced oxidative browning in various fruit crops [8,9]. Ascorbic acid is able to scavenge oxygen radicals produced when the plant tissue is wounded, therefore protecting the cells from oxidative injury.…”

Section: Resultsmentioning

confidence: 99%

Phenolic Exudation Control and Establishment of In vitro Strawberry (Fragaria × Ananassa) cv. Chandler

Mir

Rani

Ahmad

et al. 2019

CJAST

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The rate of strawberry propagation through conventional technique is quite low and it is difficult to maintain planting material during the summer months under Bihar condition. Further, importing mother plants adds to the production cost. In vitro micro propagation has emerged as a potential alternative for supplying planting material for strawberry. Two type of explants viz., runner tip and nodal segment were used for the study. Phenol exudation was the major problem during establishment which caused death of majority explants. In our experiment, almost no phenolic exudation (+) and maximum percent regeneration for runner tip (55.2 ± 0.52%) and nodal segment (58.1 ± 0.54%) was observed when MS medium was supplemented with ascorbic acid 200 mg per liter. Phenolic exudation was recorded highest (++++) under control when no antioxidants were supplemented. Minimum number of days for runner tips (8.4 ± 0.23) and nodal segments (10.3 ± 0.33) taken for shoot proliferation was observed when MS medium was supplemented with activated charcoal 300 mg and 200 mg per liter, respectively. Though all other antioxidants used in our study including citric acid, PVP and activated charcoal significantly reduced oxidative browning, ascorbic acid was found to be most effective antioxidant in controlling lethal browning during in vitro establishment of strawberry. This protocol has a potential for allowing a large scale multiplication of this important crop.

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Section: Resultsmentioning

confidence: 99%

Phenolic Exudation Control and Establishment of In vitro Strawberry (Fragaria × Ananassa) cv. Chandler

Mir

Rani

Ahmad

et al. 2019

CJAST

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“…From previous research, it was reported that 16.7 roots/shoot were established on MS medium with 2.69 µM NAA (Hoesen, 2009), the ½ MS medium with 0.27 µM NAA was more suitable for rooting; 19 roots/shoot and 5.2 cm root length of Lilium longiflorum were resulted in MS medium with NAA in 2 mg/l (Mir et al, 2012), the individual shoots were successfully planted in MS medium augmented with 2.46 µM IBA for root formation (Saetiew and Umamanit, 2015), 6.5 roots/shoot were established in MS medium with 12.08 µM NAA (Fauziah et al, 2019), and 13.3% root formation was determined on MS medium added by 5.37 µM NAA . In the experiment, Chu N6 medium hormone free, supplemented with 1% activated charcoal, was successfully induced 2.9 to 5.8 roots/shoot with 7.4 to 18.3 mm root length.…”

Section: Discussion Discussion Discussionmentioning

confidence: 96%

Potential utilization of filaments and ovules as explant sources in in vitro propagation of Lilium sp.

WINARTO,

KARTIKANINGRUM,

RACHMAWATI

et al. 2024

Not Bot Horti Agrobo

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Lilies are important cut and pot flowers locally and globally; however, qualified planting materials from in vitro culture are still limited. The culture generally uses scales and leaves, while filaments and ovules are rarely utilized. Filaments and ovules of EXO CF/E, RDF CG/E, and DZ CG/E genotypes of Lilium sp. were explored for their potential to establish in vitro propagation protocol using different Chu N6 medium and their modifications. The 9.9 shoots/explant from 1.5 regenerative explants/replication of RDF CG/E filaments was proven on Chu N6 medium containing 6.79 µM 2,4-dichlorophenoxyacetic acid (2,4-D), 2.27 µM thidiazuron (TDZ), and 90 g L-1 sucrose; 3.3 regenerative explants/replication and 8.4 shoots/explant of EXO CF/E ovules were determined on Chu N6 medium with 2.26 µM 2,4-D, 6.81 µM TDZ and 90 g L-1 sucrose; and slightly improved on Chu N6 macro elements, full vitamins of MS basal medium with 3.39 µM 2,4-D, 3.41 µM TDZ, 150 ml L-1 coconut water (CW) and 60 g L-1 sucrose. The shoots were proliferated on the established media. The shoots of EXO CF/E, RDF CG/E and DZ CG/E genotypes produced varied leaves/shoot, leaf length, leaf width, roots/shoot, root length and bulb diameter on Chu N6 medium hormone free with 1.5% AC, followed by hardening them in a glass house for one month. The hardened-plantlets were acclimatized in plastic pots containing charcoal husk and bamboo compost (1:1) with 39.8-63.6% survivability. The protocol has the potential to be applied to other genotypes or lilies by specific improvements at the acclimatization stage.

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“…Additionally, medium supplemented with IBA was found to deliver the most favorable results for rooting activity in L . longiflorum [ 21 , 47 ] and L . napalense [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

Application of machine learning in in vitro propagation of endemic Lilium akkusianum R. Gämperle

Tütüncü

2024

PLoS ONE

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A successful regeneration protocol was developed for micropropagation of Lilium akkusianum R. Gämperle, an endemic species of Türkiye, from scale explants. The study also aimed to evaluate the effects of Meta-Topolin (mT) and N6-Benzyladenine (BA) on in vitro regeneration. The Murashige and Skoog medium (MS) supplemented with different levels of α-naphthaleneacetic acid (NAA)/BA and NAA/mT were used for culture initiation in the darkness. The highest callus rates were observed on explants cultured on MS medium with 2.0 mg/L NAA + 0.5 mg/L mT (83.31%), and the highest adventitious bud number per explant was 4.98 in MS medium with 0.5 mg/L NAA + 1.5 mg/L mT. Adventitious buds were excised and cultured in 16/8 h photoperiod conditions. The highest average shoot number per explant was 4.0 in MS medium with 2.0 mg/L mT + 1.0 mg/L NAA. Shoots were rooted with the highest rate (90%) in the medium with the 1.0 mg/L IBA, and the highest survival rate (87.5%) was recorded in rooted shoots in the same medium. The ISSR marker system showed that regenerated plantlets were genetically stable. Besides traditional tissue culture techniques used in the current study, the potential for improving the effectiveness of L. akkusianum propagation protocols by incorporating machine learning methodologies was evaluated. ML techniques enhance lily micropropagation by analyzing complex biological processes, merging with traditional methods. This collaborative approach validates current protocols, allowing ongoing improvements. Embracing machine learning in endemic L. akkusianum studies contributes to sustainable plant propagation, promoting conservation and responsible genetic resource utilization in agriculture.

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Technique to minimize phenolics in walnut in vitro culture initiation

Cited by 6 publications

References 0 publications

Phenolic Exudation Control and Establishment of In vitro Strawberry (Fragaria × Ananassa) cv. Chandler

Phenolic Exudation Control and Establishment of In vitro Strawberry (Fragaria × Ananassa) cv. Chandler

Potential utilization of filaments and ovules as explant sources in in vitro propagation of Lilium sp.

Application of machine learning in in vitro propagation of endemic Lilium akkusianum R. Gämperle

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