Techniques applied to the study of microbial impact on building materials

Welton, Ryan G.; Silva, M. Ribas; Gaylarde, Christine C.; Herrera, Liz Karen; Anleo, X.; Belie, Nele De; Modrý, S.

doi:10.1007/bf02482255

Cited by 13 publications

(10 citation statements)

References 6 publications

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“…As mentioned, one of the ways for quantitative assessment of biofilm is the use of the sedimentation chambers. Welton et al (2005) used the Helber's chamber, which is usually used for the counting of bacteria (Collins & Lyne, 2004). This technique is even more suitable for enumeration of algal cells or fungal spores, given that it can be performed at higher magnification.…”

Section: Discussionmentioning

confidence: 99%

“…However, the quantification of these parameters in biofilm that is exposed to air is quite rare, in general. The counting of cyanobacterial and algal cells on opaque surfaces can be, e.g., carried out directly by using the epifluorescence microscope (Welton et al, 2005), which is a fast and non-destructive method based on chlorophyll fluorescence. The abundance of microorganisms can also be assessed using certain scales (based on the frequency in which they appear in the sample).…”

Section: Introductionmentioning

confidence: 99%

“…Some researchers reported the use of different sedimentation chambers (often used in phytoplankton analyses) for determining the cyanobacterial and algal abundance in biofilm samples. The preparation of biofilm for sedimentation implies proper biofilm sampling from the substrate, homogenization in water, followed by sedimentation and counting of cyanobacterial and algal cells (Welton et al, 2005). One of the novel approaches that is easy-to-use and represents a fast method for biofilm characterization (qualitative and quantitative) and detection of biofilm community structure changes is flow cytometry.…”

Section: Introductionmentioning

confidence: 99%

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Cyanobacterial and algal abundance and biomass in cave biofilms and relation to environmental and biofilm parameters

Popović¹,

Nikolić²,

Jovanović³

et al. 2019

IJS

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Due to life in extreme environments, cyanobacteria and algae from cave biofilms that form at the entrances or deep inside the cave around artificial lights are of increasing interest to many scientists. It is well-known that many phototrophic microorganisms are first to colonize exposed substrata and produce the organic matter on which other biofilm constituents relly. Many studies dealing with phototrophic microorganisms from biofilms focus on the diversity and community composition of cyanobacteria and algae, while quantitative assessments are rarely implemented. Biofilm sampling was conducted in Degurić and Vernjikica Cave located in Western and Eastern Serbia, respectively. Ecological parameters (temperature, relative humidity, light intensity) and distance from the entrance were measured. Additionally, chlorophyll content, as well as biofilm parameters (water content, organic and inorganic matter) were determined. The abundance of phototrophic microorganisms was assessed on microscope slides which contained 1 mg of biofilm that was dehydrated for a short period of time and homogenized prior to slide preparation, and then rehydratated. The biomass of recorded cyanobacterial and algal taxa was calculated by applying geometric approximations and standard mathematical formulas. In Degurić Cave, at the sampling site where the highest biomass was documented, the higher diversity, water content and chlorophyll values were also recorded, while in Vernjikica Cave a high content of organic matter was documented. According to the multivariate analyses performed, the biomass of simple trichal Cyanobacteria, Bacillariophyta, and Xanthophyta was positively correlated with the content of organic matter in biofilm and light intensity, while coccoid and heterocytous Cyanobacteria and Chlorophyta

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cyanobacterial and algal abundance and biomass in cave biofilms and relation to environmental and biofilm parameters

Popović¹,

Nikolić²,

Jovanović³

et al. 2019

IJS

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“…The nonaggressive adhesive tape method was used for sampling phototrophic microorganisms and micromycetes for direct observation of SAB 12 . The non-destructive adhesive tape method was carried out by gently applying a strip of adhesive tape to the tombstone surface being studied, which was then removed to sterile glass microscope slides and kept in sterile conditions prior to laboratory processing.…”

Section: Microbiological Sampling and Analysesmentioning

confidence: 99%

“…Microscopy (light and SEM) is the most suitable tool for analysing SAB and bioderioration process 12 . Optical microscopy showed the presence of SAB-forming photoautotrophs and filamentous fungi.…”

Section: Microscopic Observationsmentioning

confidence: 99%

Biodiversity's Hidden Treasure:Biodeteriorated Archaeological Tombstones of Serbia

et al. 2017

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In the present study, biological colonization on medieval tombstones from archaeological sites in Serbia has been investigated. Chemical analyses showed that the stone substrata were mostly of calcium carbonate, which is highly bioreceptive. Large areas of tombstones were covered with epilithic lichenized fungi and mosses, and microbiological analyses showed the presence of micromycetes and cyanobacteria. The dominant group of fungi recorded on tombstone surfaces was microcolonial fungi, now recognized as primary colonizers of stone substrata.

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Use of in situ and confocal Raman spectroscopy to study the nature and distribution of carotenoids in brown patinas from a deteriorated wall painting in Marcus Lucretius House (Pompeii)

et al. 2011

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Colonisation of wall paintings by microorganisms and other organisms is a well-known problematic phenomenon. Besides taxonomic identification of the biodeteriogen, it is essential to evaluate the consequences of the colonisation, e.g., unsightly coloured patinas. This work proposes new methodology for characterisation of the nature of the main carotenoids and their distribution in brown stains or patinas of a deteriorated wall painting on the north wall of the atrium of Marcus Lucretius House (Pompeii, Italy). Characterisation of the brown patinas and surrounding areas (plaster and polychromy) from the wall painting started with in situ screening using, mainly, a portable Raman instrument with a handheld FTIR (DRIFTS sampling interface) in order to select the sampling areas suitable for further analysis in the laboratory. Two wall painting fragments were then analysed in the laboratory in two steps. First, microscopic observations (SEM and phase-contrast microscopy) were used to determine whether biodeteriogens were present in the samples. In a second step, confocal Raman microscopy (785 and 514 nm excitation lasers) was used to characterise the main biogenic compounds of the brown stains. Because of the resonance Raman effect (514 nm excitation laser), it was possible to obtain reliable Raman features to assign not only the nature of the main biogenic pigments (carotenoids) present in the stains, but also their spatial conformation. Moreover, Raman confocal applications, for example, Raman imaging and depth profiling were also used in a first attempt to determine the distribution of biosynthesised carotenoids in the stains, and to determine the thickness of the brown patinas.

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Techniques applied to the study of microbial impact on building materials

Cited by 13 publications

References 6 publications

Cyanobacterial and algal abundance and biomass in cave biofilms and relation to environmental and biofilm parameters

Cyanobacterial and algal abundance and biomass in cave biofilms and relation to environmental and biofilm parameters

Biodiversity's Hidden Treasure:Biodeteriorated Archaeological Tombstones of Serbia

Use of in situ and confocal Raman spectroscopy to study the nature and distribution of carotenoids in brown patinas from a deteriorated wall painting in Marcus Lucretius House (Pompeii)

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