Techniques for the brucellosis laboratory

Edelsten, R. M.

doi:10.1007/bf00402562

Cited by 13 publications

(10 citation statements)

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“…Positive reactors were defined as animals with agglutination for the RBT and iELISA with S/P value > 80. At necropsy, samples were taken from aborted foetuses, and from adults (lymph nodes, milk samples), which were then submitted to the UP Bacteriology BSL 2+ laboratory for culture on FM, as described by the World Organisation for Animal Health (OIE) [ 22 ], and modified CITA medium [ 21 ]. Suspect colonies were stained with modified Ziehl–Nielsen (Stamp) and oxidase, urease, and catalase tests were performed and confirmed to species level using PCR assays [ 23 ].…”

Section: Methodsmentioning

confidence: 99%

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Investigation of Brucella melitensis in Sable Antelope (Hippotragus niger) in South Africa

et al. 2020

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In this study, Brucella melitensis infection in sable antelope (Hippotragus niger) was investigated on two wildlife ranches in South Africa over a 12-year period in order to determine the origin of the outbreaks and the role of livestock in maintaining the disease. Retrospective data were obtained from farm records and interviews as well as samples tested from different disease scenarios and clinical settings. On one ranch, 10 of 74 sable tested seropositive for B. melitensis in 2004 but were certified clear of infection after no further brucellosis cases were detected following repeated serological tests and culling over a five-year period. Recrudescence occurred in 2013 (7 of 187 brucellosis positives) and in 2014 (one positive), with persistent, latent infection being the most reasonable explanation. In a second case study, linked to the first one through a common vendor, 15 of 80 sable tested positive in 2016, some five years after the acquisition of the animals from a putative source. Brucella melitensis biovar 1 and/or 3 were isolated from each outbreak on both ranches. Both outbreaks resulted in substantial losses for the owners, arising from testing and culling and significant resource expenditure by the state. The study identified the diagnostic challenges for identifying and resolving disease outbreaks in wildlife, the persistence of B. melitensis in sable, the risks associated with animal movements, and the need for a wildlife-sensitive disease control scheme. Although the actual source of infection could not be identified, the investigation points away from local livestock as a source of ongoing infection while the persistent infection is consistent with the disease circulating within small, ranched populations and being spread through the keeping and trading of high-value animals. The implications of the study findings to disease control in wildlife are discussed.

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Section: Methodsmentioning

confidence: 99%

“…Suspect colonies were stained with modified Ziehl–Nielsen (Stamp) and oxidase, urease, and catalase tests were performed and confirmed to species level using PCR assays [ 23 ]. Biotyping (excluding phages) was undertaken as indicated by Edelsten [ 22 ] and OIE [ 23 ] at the ARC-OVR laboratory.…”

Section: Methodsmentioning

confidence: 99%

Investigation of Brucella melitensis in Sable Antelope (Hippotragus niger) in South Africa

et al. 2020

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“…Serum was separated and screened for anti -Brucella antibodies along with positive and negative control sera using Rose Bengal Plate Test (RBPT) (13) and IgM -ELISA (NovaLisa, GmbH, Germany) as described previously (14). The samples that gave positive reactions for both tests were considered as positive due to the variation in the sensitivity and specificity of each test (15).…”

Section: Brucella Detectionmentioning

confidence: 99%

Epidemiological Investigation of Human Brucellosis in Pakistan

Ali

Nawaz

Akhtar

et al. 2018

Jundishapur J Microbiol

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Background: Human brucellosis is a neglected and zoonotic disease also known as Malta fever or undulant fever. Objectives: A cross sectional study was conducted to determine the seroprevalence of brucellosis and to access the role of risk factors associated with this disease in humans of Punjab, Pakistan. Methods: A total of 250 serum samples were collected and subjected to Rose Bengal Plate Test and Enzyme Linked Immunosorbent Assay for screening of Brucella. A predesigned questionnaire was filled prior to sampling to collect data regarding socio -demographic and suspected risk factors of human brucellosis. Descriptive and bivariate statistical analysis was performed using the STATA software version 12. Results: The study showed 16% seroprevalence of brucellosis. The prevalence was statistically higher in males (24%), age group of 20 to 30 years (26.92%), rural residents, (23%) and individuals with animals at home (22.50%). Among the related risk factors, exposure to animals (OR = 1.87, 95% CI: 0.9459, 3.6973) and consuming raw milk (OR = 2.36, 95% CI: 1.1713, 4.7760) were strongly associated with the disease. Conclusions: Awareness programs in the rural population should be provided about the disease and its associated risk factors. Consuming unpasteurized milk and products should be avoided to control this disease.

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“…For this reason, laboratory test results of blood and CSF are essential to the differential diagnosis of chronic infections such as tuberculosis and Lyme disease, especially in endemic areas. The detection of specific antibodies usually confirms the diagnosis of NB in blood or CSF by enzyme-linked immunosorbent assay (ELISA) or Coombs’ test, or less commonly by positive CSF cultures [ 14 ]. Although tissue culture is the gold standard for diagnosis of brucellosis, due to its long culture period and low sensitive rate, it may delay the diagnosis of patients [ 15 , 16 ].…”

Section: Discussionmentioning

confidence: 99%

Neurobrucellosis with ischemic stroke and spinal cord involvement: a case report

et al. 2021

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Background Brucellosis is a common zoonotic disease that may have a variety of clinical manifestations when it affects the nervous system. Ischemic stroke is a rare clinical symptom, but if it is not diagnosed and treated early, it may cause more severe consequences. Case presentation We report a 38-year-old man presenting with hearing impairment for four years and sudden weakness of the right limb for two years, recurrent aphasia, and gradual weakness of bilateral lower limbs for nine months. He had bilateral positive Babinski’s sign. Cerebrospinal fluid (CSF) showed raised protein and pleocytosis. Magnetic resonance imaging (MRI) showed ischemic infarcts in the pons and extensive enhancement of spinal meninges combined with spinal cord atrophy and ischemia. The tests revealed Brucella Rose Bengal positive in serum and CSF. Brucella culture in CSF was also positive. Next-generation sequencing (NGS) of CSF revealed positive for Brucella with 105 species were detected. He showed significant improvement with antibiotics at five months follow-up. Conclusions Neurobrucellosis may mimic stroke and transverse myelitis like syndromes. NB is a treatable infectious condition and should always be considered in the differentials, especially if there are risk factors, as in our case.

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Techniques for the brucellosis laboratory

Cited by 13 publications

References 0 publications

Investigation of Brucella melitensis in Sable Antelope (Hippotragus niger) in South Africa

Investigation of Brucella melitensis in Sable Antelope (Hippotragus niger) in South Africa

Epidemiological Investigation of Human Brucellosis in Pakistan

Neurobrucellosis with ischemic stroke and spinal cord involvement: a case report

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