Techniques for the microinjection of single cells

Müller, C.; Grässmann, A.; Grassmann, Marian

doi:10.1042/bst0090585

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2011

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“…For example, R. Chambers taught Tom King how to microinject which resulted in cloning of frogs by nuclear transfer (Briggs and King, 1952). The microinjection technique was adapted by Grässmann's and Diacumakos' groups (reviewed in Müller et al, 1981) to study the effects of perturbation of intracellular events in a single cell in culture. Soon after its introduction, the micropipette was transformed into the microelectrode, thus laying foundation to electrophysiology (reviewed in Bretag, 1983; Brown and Flaming, 1986).…”

Section: After Barbermentioning

confidence: 99%

Marshall Barber and the century of microinjection: from cloning of bacteria to cloning of everything

Korzh

Strähle

2002

Differentiation

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Section: After Barbermentioning

confidence: 99%

Marshall Barber and the century of microinjection: from cloning of bacteria to cloning of everything

Korzh

Strähle

2002

Differentiation

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“…There are many different methods to transfect cells, such as cationic polymers [1], precipitation with calcium phosphate [2], lipid-based transfection [3, 4], microinjection/gene gun [5] and electroporation [6]. Among them, electroporation-based technologies have gained popularity because of their efficiency and relatively low toxicity.…”

Section: Introductionmentioning

confidence: 99%

Nucleofection induces non-specific changes in the metabolic activity of transfected cells

et al. 2011

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Transfection has become an everyday technique widely used for functional studies in living cells. The choice of the particular transfection method is usually determined by its efficiency and toxicity, and possible functional consequences specific to the method used are normally overlooked. We describe here that nucleofection, a method increasingly used because of its convenience and high efficiency, increases the metabolic rate of some cancer cells, which can be misleading when used as a measure of proliferation. Moreover, nucleofection can alter the subcellular expression pattern of the transfected protein. These undesired effects are independent of the transfected nucleic acid, but depend on the particular cell line used. Therefore, the interpretation of functional data using this technology requires further controls and caution.

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Techniques for the microinjection of single cells

Cited by 2 publications

References 2 publications

Marshall Barber and the century of microinjection: from cloning of bacteria to cloning of everything

Marshall Barber and the century of microinjection: from cloning of bacteria to cloning of everything

Nucleofection induces non-specific changes in the metabolic activity of transfected cells

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