Techniques to stimulate and interrogate cell–cell adhesion mechanics

Yang, Ruiguo; Broussard, Joshua A.; Green, Kathleen J.; Espinosa, Horacio D.

doi:10.1016/j.eml.2017.12.002

Cited by 17 publications

(7 citation statements)

References 213 publications

(215 reference statements)

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“…[116] It has long been thought that DSMs have no role in mechanosensing, but recent studies using FRET-based tension sensors showed that the desmosomal cadherin desmoglein 2 and the plakin protein desmoplakin can bear mechanical tension, thus opening the possibility that DSMs can serve as sites of mechanotransduction. [116,117] By analogy to the role of plectin in force modulation by HDs, desmoplakin in DSMs might initiate mechanosignaling after relief of its auto-inhibited non-canonical SH3 domain by mechanical force [67] and oppose force generation by AJs [118] (Figure 2). Moreover, DSM assembly requires the presence of AJs, [119] again similar to the role of FAs in formation of HDs.…”

Section: Force Distribution Between Cell-extracellular Matrix and Celmentioning

confidence: 99%

Crosstalk between Cell Adhesion Complexes in Regulation of Mechanotransduction

2020

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Physical forces regulate numerous biological processes during development, physiology, and pathology. Forces between the external environment and intracellular actin cytoskeleton are primarily transmitted through integrin-containing focal adhesions and cadherin-containing adherens junctions. Crosstalk between these complexes is well established and modulates the mechanical landscape of the cell. However, integrins and cadherins constitute large families of adhesion receptors and form multiple complexes by interacting with different ligands, adaptor proteins, and cytoskeletal filaments. Recent findings indicate that integrin-containing hemidesmosomes oppose force transduction and traction force generation by focal adhesions. The cytolinker plectin mediates this crosstalk by coupling intermediate filaments to the actin cytoskeleton. Similarly, cadherins in desmosomes might modulate force generation by adherens junctions. Moreover, mechanotransduction can be influenced by podosomes, clathrin lattices, and tetraspanin-enriched microdomains. This review discusses mechanotransduction by multiple integrin-and cadherin-based cell adhesion complexes, which together with the associated cytoskeleton form an integrated network that allows cells to sense, process, and respond to their physical environment.

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Section: Force Distribution Between Cell-extracellular Matrix and Celmentioning

confidence: 99%

Crosstalk between Cell Adhesion Complexes in Regulation of Mechanotransduction

2020

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“…At the same time, a suspension cell is held by a holding pipette with negative pressure and an injection needle with a positive pressure performing microinjections ( Figure 6 d). Additionally, the movement of the needle during microinjection is shown in Figure 6 e. Various mechanical properties of cells can be measured using this technique, such as investigating membrane elasticity, single-cell mechanotransduction study, molecular adhesion measurements between cell pairs, and quantifying mechanical and material properties of single-cell and cell nuclei [ 55 ]. The MA technique was used by Dufu et al, and they investigated the effect of voxelator (GBT440; anti-polymerization and anti-sickling agent) on the deformability of sickle Red blood cells (SS RBCs).…”

Section: Single-cell Mechanical Properties For Mechanotransductionmentioning

confidence: 99%

A Review of Single-Cell Adhesion Force Kinetics and Applications

Shinde

Illath

Gupta

et al. 2021

Cells

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Cells exert, sense, and respond to the different physical forces through diverse mechanisms and translating them into biochemical signals. The adhesion of cells is crucial in various developmental functions, such as to maintain tissue morphogenesis and homeostasis and activate critical signaling pathways regulating survival, migration, gene expression, and differentiation. More importantly, any mutations of adhesion receptors can lead to developmental disorders and diseases. Thus, it is essential to understand the regulation of cell adhesion during development and its contribution to various conditions with the help of quantitative methods. The techniques involved in offering different functionalities such as surface imaging to detect forces present at the cell-matrix and deliver quantitative parameters will help characterize the changes for various diseases. Here, we have briefly reviewed single-cell mechanical properties for mechanotransduction studies using standard and recently developed techniques. This is used to functionalize from the measurement of cellular deformability to the quantification of the interaction forces generated by a cell and exerted on its surroundings at single-cell with attachment and detachment events. The adhesive force measurement for single-cell microorganisms and single-molecules is emphasized as well. This focused review should be useful in laying out experiments which would bring the method to a broader range of research in the future.

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“…In recent years, surface sensitive techniques such as quartz crystal microbalance (QCM) or surface plasmon resonance (SPR) have been developed, as well as various passive (including particle displacement, particle deformations due to strain, stress-based FRET sensors, etc.) and active (including atomic force microscopy (AFM) and optical and magnetic tweezers) measurement techniques [13,14]. Of those techniques, AFM offers a unique combination of scale (nm to µm) and force (pN to µN), while enabling measurements in ambient conditions, (bio)chemical modifications of the probe, and combination with optical microscopy [15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Single-Cell Probe Force Studies to Identify Sox2 Overexpression-Promoted Cell Adhesion in MCF7 Breast Cancer Cells

Iturri

Weber

Vivanco

et al. 2020

Cells

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The replacement of the cantilever tip by a living cell in Atomic Force Microscopy (AFM) experiments permits the direct quantification of cell–substrate and cell–cell adhesion forces. This single-cell probe force measurement technique, when complemented by microscopy, allows controlled manipulation of the cell with defined location at the area of interest. In this work, a setup based on two glass half-slides, a non-fouling one with bacterial S-layer protein SbpA from L. sphaericus CMM 2177 and the second with a fibronectin layer, has been employed to measure the adhesion of MCF7 breast cancer cells to fibronectin films (using SbpA as control) and to other cells (symmetric vs. asymmetric systems). The measurements aimed to characterize and compare the adhesion capacities of parental cells and cells overexpressing the embryonic transcription factor Sox2, which have a higher capacity for invasion and are more resistant to endocrine therapy in vivo. Together with the use of fluorescence techniques (epifluorescence, Total Internal Fluorescence Microscopy (TIRF)), the visualization of vinculin and actin distribution in cells in contact with fibronectin surfaces is enabled, facilitating the monitoring and quantification of the formation of adhesion complexes. These findings demonstrate the strength of this combined approach to assess and compare the adhesion properties of cell lines and to illustrate the heterogeneity of adhesive strength found in breast cancer cells.

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Techniques to stimulate and interrogate cell–cell adhesion mechanics

Cited by 17 publications

References 213 publications

Crosstalk between Cell Adhesion Complexes in Regulation of Mechanotransduction

Crosstalk between Cell Adhesion Complexes in Regulation of Mechanotransduction

A Review of Single-Cell Adhesion Force Kinetics and Applications

Single-Cell Probe Force Studies to Identify Sox2 Overexpression-Promoted Cell Adhesion in MCF7 Breast Cancer Cells

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