In the modern agricultural landscape, realizing data’s full potential requires a unified infrastructure where stakeholders collaborate and share their data to gain insights and create business value. The agricultural data ecosystem (ADE) serves as a crucial socio-technical infrastructure, aggregating diverse data from various platforms and, thus, advertising sustainable agriculture and digitalization. Establishing trustworthy data sharing and exchange in agro-food value chains involves socioeconomic and technological elements addressed by the agricultural data space (ADS) and its trust principles. This paper outlines key challenges to data sharing in agro-food chains impeding ADE establishment based on the review of 27 studies in scientific literature. Challenges mainly arise from stakeholders’ mistrust in the data-sharing process, inadequate data access and use policies, and unclear data ownership agreements. In the ADE context, interoperability is a particularly challenging topic for ensuring the long-term sustainability of the system. Considering these challenges and data space principles and building blocks, we propose a set of design principles for ADS design and implementation that aim to mitigate the adverse impact of these challenges and facilitate agricultural data sharing and exchange.