Technologies to improve the sensitivity of existing chromatographic methods used for bioanalytical studies

Chen, Zhendong; Gao, Yuxiong; Zhong, Dafang

doi:10.1002/bmc.4798

Cited by 14 publications

(7 citation statements)

References 87 publications

(107 reference statements)

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“…In fact, there is a great biological variation in these matrices, and the direct recovery of the drug from the tissues is difficult. Thus, the use of skin layer matrices spiked with the analyte is the procedure recommended by the health guidelines and was adopted in this study (Chen, Gao, & Zhong, 2020;FDA, 2018;van Nuland, Rosing, Schellens, & Beijnen, 2020). Note.…”

Section: Methods Validationmentioning

confidence: 99%

Development of a reversed‐phase high‐performance liquid chromatographic method for the determination of propranolol in different skin layers

Cunha-Filho

Rocha

Duarte

et al. 2020

Biomedical Chromatography

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The aim of this work was to develop and validate an analytical method using HPLC for the determination of propranolol in the different layers of the skin to be used in kinetic studies of skin permeation. The development of the method was based on the suitability of the chromatogram, and the validation followed the international health regulation for bioanalytical methods. In addition, the method was tested in an in vitro permeation assay using porcine skin. The drug was determined using an RP‐C18 column at 30°C, a mobile phase comprising acidic aqueous phase:acetonitrile (75:25 v/v), at a flow rate of 1.0 mL min−1, and UV detection at 290 nm. The method was demonstrated to be selective against skin contaminants, linear in a wide range of concentrations (3–20 μg mL−1), sensitive enough to quantify less than 0.1% of the drug dosage in skin matrices, and precise regardless of analysis variations such as day of analysis, analyst, or equipment. In addition, the method presented a high drug extraction capacity greater than 90% for all skin layers (stratum corneum, hair follicle, and remaining skin). Finally, the method was successfully tested in skin permeation assays, proving its value in the development of topical formulations containing propranolol.

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Section: Methods Validationmentioning

confidence: 99%

Development of a reversed‐phase high‐performance liquid chromatographic method for the determination of propranolol in different skin layers

Cunha-Filho

Rocha

Duarte

et al. 2020

Biomedical Chromatography

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“…Common detectors used in LC include UV-VIS detectors and diode array detectors (DAD), which use the principle that a physical or chemical property of the sample differs from the mobile phase to achieve the separation of substances [ 75 , 76 ]. This technique has been used to detect polar compounds with high sensitivity, selectivity, and reproducibility [ 77 , 78 ]. The choice of mobile phase and chromatographic column are the main two important parts.…”

Section: Analytical Technologies For Hormones In Cosmeticsmentioning

confidence: 99%

Advances on Hormones in Cosmetics: Illegal Addition Status, Sample Preparation, and Detection Technology

Wang

et al. 2023

Molecules

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Owing to the rapid development of the cosmetic industry, cosmetic safety has become the focus of consumers’ attention. However, in order to achieve the desired effects in the short term, the illegal addition of hormones in cosmetics has emerged frequently, which could induce skin problems and even skin cancer after long-term use. Therefore, it is of great significance to master the illegal addition in cosmetics and effectively detect the hormones that may exist in cosmetics. In this review, we analyze the illegally added hormone types, detection values, and cosmetic types, as well as discuss the hormone risks in cosmetics for human beings, according to the data in unqualified cosmetics in China from 2017 to 2022. Results showed that although the frequency of adding hormones in cosmetics has declined, hormones are still the main prohibited substances in illegal cosmetics, especially facial masks. Because of the complex composition and the low concentration of hormones in cosmetics, it is necessary to combine efficient sample preparation technology with instrumental analysis. In order to give the readers a comprehensive overview of hormone analytical technologies in cosmetics, we summarize the advanced sample preparation techniques and commonly used detection techniques of hormones in cosmetics in the last decade (2012–2022). We found that ultrasound-assisted extraction, solid phase extraction, and microextraction coupled with chromatographic analysis are still the most widely used analytical technologies for hormones in cosmetics. Through the investigation of market status, the summary of sample pretreatment and detection technologies, as well as the discussion of their development trends in the future, our purpose is to provide a reference for the supervision of illegal hormone residues in cosmetics.

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“…Derivatization is not always desirable, but sometimes necessary to solve the problem of determining of target analytes from biomatrix (Chen, Gao, & Zhong, 2020). Derivatization may solve some of the problems, but meanwhile it complicates the analytical procedure by adding more operations to the sample preparation process.…”

Section: Future Perspectivesmentioning

confidence: 99%

Derivatization procedures and their analytical performances for HPLC determination in bioanalysis

David

Moldoveanu

Galaon

2020

Biomedical Chromatography

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Derivatization, or chemical structure modification, is often used in bioanalysis performed by liquid chromatography technique in order to enhance detectability or to improve the chromatographic performance for the target analytes. The derivatization process is discussed according to the analytical procedure used to achieve the reaction between the reagent and the target compounds (containing hydroxyl, thiol, amino, carbonyl and carboxyl as the main functional groups involved in derivatization). Important procedures for derivatization used in bioanalysis are in situ or based on extraction processes (liquid-liquid, solid-phase and related techniques) applied to the biomatrix. In the review, chiral, isotope-labeling, hydrophobicity-tailored and post-column derivatizations are also included, based on representative publications in the literature during the last two decades. Examples of derivatization reagents and brief reaction conditions are included, together with some bioanalytical applications and performances (chromatographic conditions, detection limit, stability and sample biomatrix).

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Technologies to improve the sensitivity of existing chromatographic methods used for bioanalytical studies

Cited by 14 publications

References 87 publications

Development of a reversed‐phase high‐performance liquid chromatographic method for the determination of propranolol in different skin layers

Development of a reversed‐phase high‐performance liquid chromatographic method for the determination of propranolol in different skin layers

Advances on Hormones in Cosmetics: Illegal Addition Status, Sample Preparation, and Detection Technology

Derivatization procedures and their analytical performances for HPLC determination in bioanalysis

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