TEER Measurement Techniques for In Vitro Barrier Model Systems

Srinivasan, Balaji; Kolli, Aditya Reddy; Esch, Mandy B.; Abaci, Hasan Erbil; Shuler, Michael L.; Hickman, James J.

doi:10.1177/2211068214561025

Cited by 1,630 publications

(1,508 citation statements)

References 140 publications

(179 reference statements)

Supporting

Mentioning

1,419

Contrasting

Unclassified

Order By: Relevance

“…Taking these points into consideration, we posit that the observed R cl fluctuation of Figure 3a (top) is attributed to changes in the cell/cell paracellular distance, due to changes in packing of the cells on the active area of the transistor channel during high flow. In fact, measured changes in the impedance spectra were observed in the frequency range (ƒ(Hz) ⩽ 400 Hz) that are commonly attributed to the paracellular resistance 50,51 . The aforementioned changes in the R cl are shown in Supplementary Figure S6, together with the fitting curves used for the extrapolation of R cl and C cl reported in Figure 3a.…”

Section: Results and Discussion Flow Shear Stress Mechanical Stimulationmentioning

confidence: 99%

Organic transistor platform with integrated microfluidics for in-line multi-parametric in vitro cell monitoring

et al. 2017

View full text Add to dashboard Cite

Future drug discovery and toxicology testing could benefit significantly from more predictive and multi-parametric readouts from in vitro models. Despite the recent advances in the field of microfluidics, and more recently organ-on-a-chip technology, there is still a high demand for real-time monitoring systems that can be readily embedded with microfluidics. In addition, multi-parametric monitoring is essential to improve the predictive quality of the data used to inform clinical studies that follow. Here we present a microfluidic platform integrated with in-line electronic sensors based on the organic electrochemical transistor. Our goals are twofold, first to generate a platform to host cells in a more physiologically relevant environment (using physiologically relevant fluid shear stress (FSS)) and second to show efficient integration of multiple different methods for assessing cell morphology, differentiation, and integrity. These include optical imaging, impedance monitoring, metabolite sensing, and a wound-healing assay. We illustrate the versatility of this multi-parametric monitoring in giving us increased confidence to validate the improved differentiation of cells toward a physiological profile under FSS, thus yielding more accurate data when used to assess the effect of drugs or toxins. Overall, this platform will enable high-content screening for in vitro drug discovery and toxicology testing and bridges the existing gap in the integration of in-line sensors in microfluidic devices.

show abstract

Section: Results and Discussion Flow Shear Stress Mechanical Stimulationmentioning

confidence: 99%

Organic transistor platform with integrated microfluidics for in-line multi-parametric in vitro cell monitoring

et al. 2017

View full text Add to dashboard Cite

show abstract

“…This finding encourages sufficient Caco-2 monolayer integrity despite of decreasing TEER values. A general minimum limit of TEER indicating a nonsufficient cell monolayer integrity is hard to determine as TEER values vary depending on used equipment [33] and other factors such as temperature and used media [34]. In addition, decrease in TEER is proportional with an increased electron flux through cell monolayers.…”

Section: Discussionmentioning

confidence: 99%

Sustained Release for St. John?s Wort: A Rational Idea?

Disch¹,

Forsch²,

Siewert³

et al. 2017

J Bioequiv Availab

View full text Add to dashboard Cite

“…9A) and measured transepithelial electrical resistance (TEER) upon spontaneous differentiation. TEER is a widely accepted quantitative technique for measuring the integrity of tight junction dynamics in cultured endothelial and epithelial monolayers 41. Similar to the increased intestinal permeability in CerS2 null mice, CerS2 knockdown Caco‐2 cells displayed lower TEER levels during differentiation (Fig.…”

Section: Resultsmentioning

confidence: 84%

Ablation of ceramide synthase 2 exacerbates dextran sodium sulphate‐induced colitis in mice due to increased intestinal permeability

Kim

Volpert

Shin

et al. 2017

J Cellular Molecular Medi

View full text Add to dashboard Cite

Ceramides mediate crucial cellular processes including cell death and inflammation and have recently been implicated in inflammatory bowel disease. Ceramides consist of a sphingoid long‐chain base to which fatty acids of various length can be attached. We now investigate the effect of alerting the ceramide acyl chain length on a mouse model of colitis. Ceramide synthase (CerS) 2 null mice, which lack very‐long acyl chain ceramides with concomitant increase of long chain bases and C16‐ceramides, were more susceptible to dextran sodium sulphate‐induced colitis, and their survival rate was markedly decreased compared with that of wild‐type littermates. Using mixed bone‐marrow chimeric mice, we showed that the host environment is primarily responsible for intestinal barrier dysfunction and increased intestinal permeability. In the colon of CerS2 null mice, the expression of junctional adhesion molecule‐A was markedly decreased and the phosphorylation of myosin light chain 2 was increased. In vitro experiments using Caco‐2 cells also confirmed an important role of CerS2 in maintaining epithelial barrier function; CerS2‐knockdown via CRISPR‐Cas9 technology impaired barrier function. In vivo myriocin administration, which normalized long‐chain bases and C16‐ceramides of the colon of CerS2 null mice, increased intestinal permeability as measured by serum FITC‐dextran levels, indicating that altered SLs including deficiency of very‐long‐chain ceramides are critical for epithelial barrier function. In conclusion, deficiency of CerS2 influences intestinal barrier function and the severity of experimental colitis and may represent a potential mechanism for inflammatory bowel disease pathogenesis.

show abstract

TEER Measurement Techniques for In Vitro Barrier Model Systems

Cited by 1,630 publications

References 140 publications

Organic transistor platform with integrated microfluidics for in-line multi-parametric in vitro cell monitoring

Organic transistor platform with integrated microfluidics for in-line multi-parametric in vitro cell monitoring

Sustained Release for St. John?s Wort: A Rational Idea?

Ablation of ceramide synthase 2 exacerbates dextran sodium sulphate‐induced colitis in mice due to increased intestinal permeability

Contact Info

Product

Resources

About