Tektin 2 is required for central spindle microtubule organization and the completion of cytokinesis

Durcan, Thomas M.; Halpin, Elizabeth S.; Rao, Trisha; Collins, Nicholas S.; Tribble, Emily; Hornick, Jessica E.; Hinchcliffe, Edward H.

doi:10.1083/jcb.200711160

Cited by 28 publications

(29 citation statements)

References 45 publications

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“…Morever, mRNA encoding tektin-1 is detected in freshly isolated radial glial cells (Li et al, 2003). Finally tektin-2, which is also associated to mitotic spindles and midbody in CHO cells, is required for proper central spindle microtubule organization and cytokinesis completion (Durcan et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Distribution of EFHC1 or Myoclonin 1 in mouse neural structures

et al. 2010

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Summary EFHC1, a gene mutated in juvenile myoclonic epilepsy, encodes EFHC1, a protein with three DM10 domains and one EF-hand motif. We recently demonstrated that this molecule is a microtubule-associated protein (MAP) implicated in neuronal migration. Because some controversies persist about the precise localization in the CNS, we studied the neuroanatomical distribution of EFHC1 in mature and developing mouse brain. In the adult, low mRNA expression was detected in several brain structures such as cortex, striatum, hippocampus and cerebellum. At E16, EFHC1 mRNA was shown to be expressed in cortex and not only in cells lining ventricles.Using a purified polyclonal antibody, EFHC1 staining was observed in all cortical layers, in piriform cortex, in hippocampus and in Purkinje cells of cerebellum. In the cortex, the majority of EFHC1 positive cells correspond to neurons, however some glial cells were also stained. In agreement with a previous study, we demonstrated strong EFHC1 expression in cilia of ependymal cells lining cerebral ventricles. Moreover, at E16, the protein was observed at the borders of brain ventricles, in choroid plexus, but also, although to a lesser extent, in piriform and neocortex. In these latter structures, the pattern of expression seems to correspond to the extensions of the radial glia fibers as demonstrated by BLBP immunostaining. Finally, we confirmed that EFHC1 was also expressed and co-localized with the mitotic spindle of neural stem cells.These results confirm that EFHC1 is a protein with a likely low expression level in mouse brain but detectable both in adult and embryonic brain supporting our previous data and Abbreviations: ABC, avidin-biotin-peroxidase complex; BLBP, brain lipid-binding protein; DAB3, 3 -diaminobenzidine; FITC, fluorescein isothiocyanate; GFAP, glial fibrillary acidic protein; IgG, immunoglobulin G; JME, juvenile myoclonic epilepsy; MAP, microtubule-associated protein; MTOC, microtubule-organizing center; NeuN, neuronal nuclei; NGS, normal goat serum; NSC, neural stem cells; PBS, phosphatebuffered saline; PBSG, phosphate-buffered saline with 4.5 g/l glucose; PFA, paraformaldehyde; RRX, rhodamine Red-X; VDCC, voltagedependent calcium channel. Distribution of EFHC1 or Myoclonin 1 in mouse neural structures 197 hypothesis that EFHC1 could play an important role during brain development. As discussed, this opens the door to a new conceptual approach viewing some idiopathic generalized epilepsies as developmental diseases instead of classical channelopathies.

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Section: Discussionmentioning

confidence: 99%

Distribution of EFHC1 or Myoclonin 1 in mouse neural structures

et al. 2010

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“…Tektin 2, another member of the Tektin family, is a midbody component and is indispensable for central spindle microtubule organization (12). Our results suggested that Tektin 1 also has a similar function at the midbody during cytokinesis.…”

Section: Cep57 Recruits Tektin 1 To the Midbody And Functions With Itmentioning

confidence: 58%

“…As Cep57 directly binds to microtubules and facilitates central spindle microtubule organization (15, 16), we next investigated whether the central spindle and midbody localization of Cep57 is dependent on microtubules. We used blebbistatin, a non-muscle myosin II inhibitor, to synchronize HeLa cells to late anaphase and then added nocodazole to depolymerize microtubules (12,22). After the treatment, Cep57 was absent from the Flemming body, whereas its centrosome localization was not affected (Fig.…”

Section: Cep57mentioning

confidence: 99%

“…Thus, Cep57 may be required for cytokinesis by playing a structural role in stabilizing the microtubules and ensuring proper central spindle organization and correct midbody assembly, which ensure the accomplishment of cytokinesis (4,6,12).…”

Section: Cep57mentioning

confidence: 99%

“…Cep55, a microtubule-associated protein, localizes to the spindle midzone through association with the MKLP1 (mitotic kinesin-like protein 1)-male germ cell Rac GTPase-activating protein centralspindlin complex, which is essential for the midbody structure and successful cytokinesis (3,11). Tektin 2 organizes the central spindle and midbody microtubules, and its depletion disrupts proper midbody assembly and leads to cytokinesis failure (12).…”

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confidence: 99%

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Cep57 Protein Is Required for Cytokinesis by Facilitating Central Spindle Microtubule Organization

Zhou

et al. 2013

Journal of Biological Chemistry

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Background: Cep57 has been shown to be a centrosome protein with microtubule-binding and microtubule-bundling activities. Results: Cep57 localizes to the midbody and plays roles in central spindle microtubule organization to ensure the completion of cytokinesis. Conclusion: Cep57 is required for cytokinesis. Significance: We identified the function of Cep57 in cytokinesis as a novel factor of central spindle microtubule organization.

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Centrosome biogenesis continues in the absence of microtubules during prolonged S‐phase arrest

Collins

Hornick

Durcan

et al. 2010

Journal Cellular Physiology

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SummaryWhen CHO cells are arrested in S-phase, they undergo repeated rounds of centrosome duplication without cell cycle progression. While the increase is slow and asynchronous, the number of centrosomes in these cells does rise with time. To investigate mechanisms controlling this duplication, we have arrested CHO cells in S-phase for up to 72 hours, and coordinately inhibited new centriole formation by treatment with the microtubule poison colcemid. We find that in such cells, the pre-existing centrosomes remain, and a variable number of foci -containing α/γ-tubulin and centrin 2 -assemble at the nuclear periphery. When the colcemid is washed out, the nuclearassociated foci disappear, and cells assemble new centriole-containing centrosomes, which accumulate the centriole scaffold protein SAS-6, nucleate microtubule asters, and form functional mitotic spindle poles. The number of centrosomes that assemble following colcemid washout increases with duration of S-phase arrest, even though the number of nuclear-associated foci or pre-existing centrosomes does not increase. This suggests that during S-phase, a cryptic generative event occurs repeatedly, even in the absence of new triplet microtubule assembly. When triplet microtubule assembly is restored, these cryptic generative events become realized, and multiple centriole-containing centrosomes assemble.

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Tektin 2 is required for central spindle microtubule organization and the completion of cytokinesis

Cited by 28 publications

References 45 publications

Distribution of EFHC1 or Myoclonin 1 in mouse neural structures

Distribution of EFHC1 or Myoclonin 1 in mouse neural structures

Cep57 Protein Is Required for Cytokinesis by Facilitating Central Spindle Microtubule Organization

Centrosome biogenesis continues in the absence of microtubules during prolonged S‐phase arrest

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