Telomerase Activity in Cervical Smears

Saretzki, Gabriele; Fischer, Henrike J.; Kaufmann, I; Schewe, Christiane; Nadjari, Bahram; Blohmer, Jens-Uwe; Hauptmann, S.

doi:10.1155/2001/630972

Cited by 8 publications

(10 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results are comparable to previous studies in which hTERT expression relates to the severity of the disease [30][31][32]. [33,34]. Published studies on the subject reveal that hTERT expression and telomerase activity was reported in 91.3%, 96.5% and 100% of cervical cancer samples [35][36][37].…”

Section: Discussionsupporting

confidence: 89%

Correlation of hTERT Expression with Cervical Cytological Abnormalities and Human Papillomavirus Infection

Zejnullahu

Josifovska

Vukovik

et al. 2017

Prilozi

View full text Add to dashboard Cite

Telomerase Reverse Transcriptase (TERT) is the main catalytic sub-unit of telomerase, a reverse transcriptase enzyme. Telomerase expression is regulated at many levels, with numerous studies suggesting that up-regulation of human TERT gene (hTERT) at transcriptional level results in immortal cell phenotype associated with cancer. The aim of this study is to determine the correlation between hTERT expression and different cervical precursor lesions, as well as with cervical cancer in patients with confirmed Human papillomavirus (HPV) infection. The study included molecular analyzes on cervical samples from 214 women and matched Papanicolaou (Pap) test results. HPV detection and genotyping was performed by polymerase chain reaction (PCR) and genotyping. Quantitative real-time PCR (qRT-PCR) was performed using TaqMan probes and were calculated relative to the reference gene. Results showed significantly increased hTERT mRNA expression levels in high-grade and low-grade lesions compared to normal control samples (p<0.01) associated with 6.31 fold higher risk for developing ASC-US and 9.20 for LSIL. Strong correlation between HPV infection and hTERT expression in the high-grade lesions and cervical cancer was also observed. hTERT relative expression values showed 98% specificity and 100 % sensitivity as indicator of cervical lesions particularly for the ACS-H, HSIL and cervical cancer. In conclusion, hTERT expression correlate with the cytological grade of the cervical lesions and HPV infection and has a potential to be used as a diagnostic and prognostic marker.

show abstract

Section: Discussionsupporting

confidence: 89%

Correlation of hTERT Expression with Cervical Cytological Abnormalities and Human Papillomavirus Infection

Zejnullahu

Josifovska

Vukovik

et al. 2017

Prilozi

View full text Add to dashboard Cite

show abstract

“…Moreover, the inhibition of hTERT expression can trigger apoptosis before a significant effect on the mean telomere length is obtained. Similarly, it has been found that transfection-enforced overexpression of hTERT hTERT and mitochondrial cell death C Massard et al can reduce stress-induced cell death (Lu et al, 2001;Saretzki et al, 2001;Gorbunova et al, 2003;Luiten et al, 2003;Kang et al, 2004). According to one group, hTERT that lacks reverse transcriptase activity loses its cytoprotective activity (Zhang et al, 2003), but according to another group, hTERT mutants that lack telomerase activity still can suppress cell death induction, for instance by DNA-damaging agents (Rahman et al, 2005).…”

mentioning

confidence: 93%

hTERT: a novel endogenous inhibitor of the mitochondrial cell death pathway

et al. 2006

View full text Add to dashboard Cite

hTERT is the catalytic subunit of the telomerase and is hence required for telomerase maintenance activity and cancer cell immortalization. Here, we show that acute hTERT depletion has no adverse effects on the viability or proliferation of cervical and colon carcinoma cell lines, as evaluated within 72 h after transfection with hTERTspecific small interfering RNAs (siRNAs). Within the same time frame, hTERT depletion facilitated the induction of apoptotic cell death by cisplatin, etoposide, mitomycin C and reactive oxygen species, yet failed to sensitize cells to death induction via the CD95 death receptor. Experiments performed with p53 knockout cells or chemical p53 inhibitors revealed that p53 was not involved in the chemosensitizing effect of hTERT knockdown. However, the proapoptotic Bcl-2 family protein Bax was involved in cell death induction by hTERT siRNAs. Depletion of hTERT facilitated the conformational activation of Bax induced by genotoxic agents. Moreover, Bax knockout abolished the chemosensitizing effect of hTERT siRNAs. Inhibition of mitochondrial membrane permeabilization by overexpression of Bcl-2 or expression of the cytomegalovirus-encoded protein vMIA (viral mitochondrial inhibitor of apoptosis), which acts as a specific Bax inhibitor, prevented the induction of cell death by the combination of hTERT depletion and chemotherapeutic agents. Altogether, our data indicate that hTERT inhibition may constitute a promising strategy for facilitating the induction of the mitochondrial pathway of apoptosis.

show abstract

“…In intraepithelial neoplasia, the occurrence rate is approximately 58%. 18 Kailash et al 19 studied 154 cervical tissue samples and observed a significant correlation (P G 0.01) between telomerase activity and positive results for HPV types 16 and 18. The authors also observed relatively high telomerase levels in high-grade lesions and invasive cervical carcinoma, which is similar to the findings of other authors.…”

Section: Discussionmentioning

confidence: 98%

Survivin and Telomerase Expression in the Uterine Cervix of Women With Human Papillomavirus-Induced Lesions

Barbosa¹,

Silva

Correa³

et al. 2011

International Journal of Gynecological Cancer

View full text Add to dashboard Cite

The results suggest that mechanisms that promote both cell proliferation (telomerase activity) and cell survival (survivin expression) are active in cervical cancer and its precursor lesions. There was a negative correlation between survivin expression and the number of PCR cycles necessary to detect telomerase activity in the total sample, achieving statistical significance in patients in the CIN-3 group.

show abstract

Telomerase Activity in Cervical Smears

Cited by 8 publications

References 24 publications

Correlation of hTERT Expression with Cervical Cytological Abnormalities and Human Papillomavirus Infection

Correlation of hTERT Expression with Cervical Cytological Abnormalities and Human Papillomavirus Infection

hTERT: a novel endogenous inhibitor of the mitochondrial cell death pathway

Survivin and Telomerase Expression in the Uterine Cervix of Women With Human Papillomavirus-Induced Lesions

Contact Info

Product

Resources

About