Telomerase activity in pig granulosa cells proliferating and differentiating in vitro

Tománek, M.; Chronowska, Ewa; Kott, T.; Czerneková, V.

doi:10.1016/j.anireprosci.2007.02.003

Cited by 18 publications

(18 citation statements)

References 35 publications

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“…This observation confirms our previous results (Tománek et al, 2008), demonstrating the higher proliferative potential of less differentiated granulosa cells.…”

Section: Discussionsupporting

confidence: 82%

“…In the laboratory the ovaries were placed in sterile PBS supplemented with an antibiotic-antimycotic solution (Sigma, UK) for 20 min. Individual follicles of the desired size (small antral 1-2 mm and large antral 5-7 mm) were isolated as described earlier (Tománek et al, 2008). The number of live granulosa cells in suspension was estimated using of 0.25% trypan blue (Sigma, USA) in PBS and by counting in a hematocytometer.…”

Section: Isolation Of Porcine Granulosa Cells and In Vitro Culture Comentioning

confidence: 99%

“…We previously determined the presence of telomerase activity in pig granulosa cells obtained from small and large antral follicles (Tománek et al, 2008). However, the mechanisms of TA regulation in granulosa cells are still largely unknown.…”

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confidence: 99%

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Effect of aromatase inhibitor (fadrozole) on proliferation, estradiol production and telomerase activity in pig granulosa cells in vitro

Chronowska¹,

Tománek²,

Kott³

2009

CZECH J ANIM SCI

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ABSTRACT:The objective of the present work was to study the effect of a nonsteroidal aromatase inhibitor (fadrozole) on proliferation, estradiol production, aromatase expression and telomerase activity (TA) in pig granulosa cells (GC) from small (1-2 mm) and large (5-7 mm) follicles. The cells were treated with fadrozole for 48 h and 72 h in basal and FSH-stimulated conditions. Fadrozole caused a decrease (P < 0.05) of 3 H-thymidine incorporation in granulosa cells derived from small (1-2 mm) and large follicles (5-7 mm). The proliferative potential of small-follicle GC was significantly higher (P < 0.01) under all culture conditions. Estradiol production was suppressed (P < 0.01) in both granulosa cell populations cultured in the presence of fadrozole for 48 and 72 h. Fadrozole caused a decrease (P < 0.05) of aromatase gene expression in small-follicle granulosa cell incubated for 72 h and in large-follicle GC after 48 h of culture. Large-follicle GC were characterized by a higher (P < 0.01) level of estradiol production and aromatase gene expression. Telomerase activity decreased (P < 0.05) in large-follicle granulosa cells incubated in the presence of an aromatase inhibitor for 72 h. The TA level in large-follicle granulosa cells was higher (P < 0.01) in comparison to small-follicle GC in all culture conditions after 72 h of incubation. The results of the present study suggest the important role of telomerase in the process of follicular growth and development.

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“…This observation confirms our previous results (Tománek et al, 2008), demonstrating the higher proliferative potential of less differentiated granulosa cells.…”

Section: Discussionsupporting

confidence: 82%

Section: Isolation Of Porcine Granulosa Cells and In Vitro Culture Comentioning

confidence: 99%

See 1 more Smart Citation

Effect of aromatase inhibitor (fadrozole) on proliferation, estradiol production and telomerase activity in pig granulosa cells in vitro

Chronowska¹,

Tománek²,

Kott³

2009

CZECH J ANIM SCI

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“…Telomerase activity was the highest in the smallest examined follicles and was decreasing significantly as the follicles enlarged [42]. Interestingly, it was shown that there is no significant difference between pig small antral and large antral follicles in telomerase activity of granulosa cells [74]. Additionally, a significantly decreased telomerase activity was observed in large and atretic follicles in comparison to small and healthy follicles in rat [83].…”

Section: Telomerase Activity and Stemness In Granulosa Cellsmentioning

confidence: 79%

Plasticity of granulosa cells: on the crossroad of stemness and transdifferentiation potential

Dzafic

Štimpfel

Virant‐Klun

2013

J Assist Reprod Genet

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The ovarian follicle represents the basic functional unit of the ovary and consists of an oocyte, which is surrounded by granulosa cells (GCs). GCs play an important role in the growth and development of the follicle. They are subject to increased attention since it has recently been shown that the subpopulation of GCs within the growing follicle possesses exceptionally plasticity showing stem cell characteristics. In assisted reproduction programs, oocytes are retrieved from patients together with GCs, which are currently discarded daily, but could be an interesting subject to be researched and potentially used in regenerative medicine in the future. Isolated GCs expressed stem cell markers such as OCT-4, NANOG and SOX-2, showed high telomerase activity, and were in vitro differentiated into other cell types, otherwise not present within ovarian follicles. Recently another phenomenon demonstrated in GCs is transdifferentiation, which could explain many ovarian pathological conditions. Possible applications in regenerative medicine are also given.

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“…The GC telomeres of women with diminished ovarian reserves have been found to be significantly shorter than those of controls (Buttsa 2006). In addition, several studies have demonstrated detectable TA in the GCs of diverse species, including pigs, cattle and humans (Lavranos et al 1999, Liu & Zhu 2003, Tomanek et al 2008. There have been no reports on predicting IVF treatment outcomes via TA and TL.…”

Section: Introductionmentioning

confidence: 99%

Telomerase activity is more significant for predicting the outcome of IVF treatment than telomere length in granulosa cells

Wang¹,

Chen²,

Li³

et al. 2014

Reproduction

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Our previous study has demonstrated that luteinized granulosa cells (GCs) have the potential to proliferate and that the telomerase activity (TA) of luteinized GCs may predict the clinical outcomes of IVF treatment. However, in the field of telomere research, there have always been different opinions regarding the significance of TA and telomere length (TL). Thus, in the present study, we compared the effects of these two parameters on IVF treatment outcomes in the same individuals. TL did not differ significantly between the pregnant group and the non-pregnant group. The TA, number of retrieved oocytes and rate of blastocyst transfer were significantly higher in the pregnant group than in the non-pregnant group (0.8825 OD!mm, 12.75G2.20 and 34.48%, respectively, in the pregnant group vs 0.513 OD!mm, 11.60G0.93 and 14.89%, respectively, in the non-pregnant group (P!0.05)), while basal FSH level was lower in the pregnant group than in the non-pregnant group. The subjects did not differ with regard to ovarian stimulation or other clinical characteristics. A TA increase of 1 OD!mm increased the chance of becoming pregnant 4.769-fold (odds ratio: 5.769, 95% CI: 1.434-23.212, P!0.014). The areas under the receiver operating characteristic curves were 0.576 for TL and 0.674 for TA (PZ0.271 and P!0. 012 respectively). The corresponding cut-off points were 4.470 for TL and 0.650 OD!mm for TA. These results demonstrate that TA is a better predictor of pregnancy outcomes following IVF treatment than TL. No other clinical parameters, including age, baseline FSH level or peak oestradiol level, distinguished between the pregnant group and the non-pregnant group as effectively as TA.

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Telomerase activity in pig granulosa cells proliferating and differentiating in vitro

Cited by 18 publications

References 35 publications

Effect of aromatase inhibitor (fadrozole) on proliferation, estradiol production and telomerase activity in pig granulosa cells in vitro

Effect of aromatase inhibitor (fadrozole) on proliferation, estradiol production and telomerase activity in pig granulosa cells in vitro

Plasticity of granulosa cells: on the crossroad of stemness and transdifferentiation potential

Telomerase activity is more significant for predicting the outcome of IVF treatment than telomere length in granulosa cells

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