Telomerase expression of malignant epithelial cells correlates with Dukes' stage in colorectal cancer

Ghori, Aqeel; Usselmann, Bernhard; Ferryman, S. R.; Morris, Alan; Fraser, Ian

doi:10.1046/j.1463-1318.2002.00378.x

Cited by 15 publications

(14 citation statements)

References 20 publications

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“…In agreement with several studies addressing telomerase activity in CRC (17,18,20,22), hTERT mRNA levels have been shown to gradually increase from normal mucosa to adenomas and carcinomas (14 -16). Nonetheless, hTERT mRNA has also been detected in telomerase-negative samples (23).…”

Section: Discussionsupporting

confidence: 87%

Relationship Between Tumor and Plasma Levels of hTERT mRNA in Patients with Colorectal Cancer: Implications for Monitoring of Neoplastic Disease

Terrin

Rampazzo

Pucciarelli

et al. 2008

Clinical Cancer Research

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Purpose: Colorectal cancer (CRC) is one of the most common cancers in western countries.Identification of circulating markers for CRC would optimize early stage diagnosis and the monitoring for disease recurrence. Expression of telomerase reverse transcriptase (hTERT) is essential to the oncogenic process and might be used as a molecular marker of neoplastic disease. Experimental Design: Eighty-five CRC samples (25 stage I, 15 stage II, 15 stage III, and 30 stage IV), the available corresponding noncancerous mucosa (n = 42), and plasma collected at the time of surgery (n = 49) were analyzed. Control plasma samples were obtained from 43 age-matched healthy subjects. All hTERT transcripts (hTERT-AT) and transcripts encoding the functional protein (hTERT-FL) were quantified by real-time PCR.Results: hTERT-ATwas found to correlate with hTERT-FL (r = 0.849; P < 0.0001) mRNA levels in tumors. Both hTERT mRNAs were significantly higher in tumors than in adjacent noncancerous mucosa and both significantly increased with tumor progression (P < 0.0001). In contrast to controls, all but two plasma samples from CRC patients were positive for hTERT mRNAs. Using the cutoff value of 180 copies hTERT-AT/mL, the sensitivity and specificity of the assay for CRC detection were 92% and 100%, respectively. Furthermore, hTERT-AT mRNA levels in plasma significantly correlated with hTERT-AT mRNA levels in tumors (r = 0.702, P < 0.0001).Conclusions: These findings indicate that quantification of hTERT mRNA in plasma may be used as a marker for detection and monitoring of neoplastic colorectal disease.

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Section: Discussionsupporting

confidence: 87%

Relationship Between Tumor and Plasma Levels of hTERT mRNA in Patients with Colorectal Cancer: Implications for Monitoring of Neoplastic Disease

Terrin

Rampazzo

Pucciarelli

et al. 2008

Clinical Cancer Research

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“…Although the activity of this enzyme was recently shown to be upregulated by augmenting the gene transcription of hTERT by survivin, 34 contradictory results have been reported for the gastrointestinal tract. The majority of studies found upregulation of telomerase activity and mRNA expression in colorectal tumors, 17,[35][36][37] while others found increased activity only in late events during carcinogenesis but not in preneoplastic lesions. 38,39 In this context, contradictory results have been found in patients with UC.…”

Section: Discussionmentioning

confidence: 99%

Enhanced expression of proproliferative and antiapoptotic genes in ulcerative colitis-associated neoplasia

Švec

Musı́lková

Bryndová

et al. 2010

Inflammatory Bowel Diseases

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“…Telomerase activity is related to the Dukes' stage: patients at stage A and B showed a lower percentage of positivity than the ones at stages C or D [45,47,61,62,64,66] . Tumours localized in colon showed a higher percentage of positivity than tumours localized in the rectum [61,64] .…”

Section: Telomerase As a Colorectal Cancer Markermentioning

confidence: 99%

“…The overall survival and the disease-free survival of patients showing high telomerase activity was significantly worse than those for other patients. Telomerase activity was an independent prognosis-associated factor Ghori et al [66] , 2002 30/20 2 Lower rates of telomerase activity were detected in tumours at early stages Kawanishi-Tabata et al [69] , 2002 122 1 Telomerase activity detection is more frequent in colon than in rectal tumours at stage Ⅱ.…”

Section: Telomerase As a Colorectal Cancer Markermentioning

confidence: 99%

Telomere function in colorectal cancer

Frías

Morán²,

Juan³

et al. 2009

WJGO

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Colorectal cancer is the third most common form of cancer and the second leading cause of cancer-related death in the western world. Tumour cells acquire the hallmarks of cancer during the carcinogenic selection process. Cell immortality is one of the principal features acquired during this process which involves the stabilization of telomere length. It is achieved mainly, by telomerase activation. Thus, the discovery of telomeres and telomerase allowed an understanding of the mechanisms by which cells can become immortalized. Different studies have shown that tumour cells have shorter telomeres than nontumour cells and have detected telomerase activity in the majority of tumours. Survival studies have determined that telomere maintenance and telomerase activity are associated with poor prognosis. Taking into account all the results achieved by different groups, quantification and evaluation of telomerase activity and measurement of telomere length may be useful methods for additional biologic and prognostic staging of colorectal carcinoma.

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Telomerase expression of malignant epithelial cells correlates with Dukes' stage in colorectal cancer

Cited by 15 publications

References 20 publications

Relationship Between Tumor and Plasma Levels of hTERT mRNA in Patients with Colorectal Cancer: Implications for Monitoring of Neoplastic Disease

Relationship Between Tumor and Plasma Levels of hTERT mRNA in Patients with Colorectal Cancer: Implications for Monitoring of Neoplastic Disease

Enhanced expression of proproliferative and antiapoptotic genes in ulcerative colitis-associated neoplasia

Telomere function in colorectal cancer

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