Telomerase in saliva: An assistant marker for oral squamous cell carcinoma

Suhail, Shaista; Sonam, Manjari; Ahmad, Mohammad Kaleem; Chandra, Shaleen; Saleem, Mohammad

doi:10.4103/jomfp.jomfp_83_19

Cited by 4 publications

(2 citation statements)

References 13 publications

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“…Studies in the past show the association of genetic risk factors with malignancy, thus suggesting that genomic modifications play a significant role in the etiology of precancerous and cancerous lesions (17,18). The process of oral carcinogenesis is an exceedingly intricate and multifaceted trajectory comprising of genetic modifications accretion and, with the help of oncogenes, causes induction of proteins resulting in abnormal cell growth, augmented enzymatic (telomerase) activity encouraging cell proliferation and loss of proteins that can also inhibit the abnormal cell proliferation with the help of tumor suppressor genes (19,20).…”

Section: Introductionmentioning

confidence: 99%

Comparing Length and Telomere Expression at Oral Precancerous and Cancerous Stages

Samadi,

Suhail,

Sonam

et al. 2024

Iran J Pathol

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Background & Objective: Telomeres consist of repetitive G-rich nucleotides located at the end of each chromosome, acting as protein binding sites. The aim of this study was to examine the differences in telomere length in blood, saliva, and tissue samples at various stages of oral precancerous and cancerous lesions. Methods: Samples of blood, tissue, and saliva were collected from patients with oral precancerous and cancerous lesions. DNA extraction was performed. Then, a TRAP assay was conducted to assess and compare the telomere length and telomerase expression. Results: The levels of telomerase activity (TA) in the DNA samples ranged from 0.19 to 6.91 (2.05 + 1.37) among oral squamous cell carcinoma (OSCC) patients and from 0.17 to 4.5 (0.28 + 4.25) among precancerous patients. A significant difference was observed in TA levels between OSCC and precancerous samples ( t =3.9691, P = 0.0000). Conclusion: Assessing the telomerase activity is crucial for studying the behavior of carcinoma in the clinical setting. The augmented telomerase expression and the length of telomere contribute to OSCC progression. Hence, this study adds a diagnostic tool that can serve as a biomarker for the early detection and prognosis of OSCC.

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Section: Introductionmentioning

confidence: 99%

Comparing Length and Telomere Expression at Oral Precancerous and Cancerous Stages

Samadi,

Suhail,

Sonam

et al. 2024

Iran J Pathol

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“…Oral squamous cell carcinoma (OSCC) constitutes the majority (>90%) of oral cancers. Evidence has suggested that OSCC may Pharmaceutics 2021, 13, 574 2 of 21 have developed from precancerous lesions, such as leukoplakia and lichen planus [2]. Current oral cancer treatment modalities include surgery, radiation, and chemotherapy, depending on tumor location and stage [3].…”

Section: Introductionmentioning

confidence: 99%

Pharmacokinetic Model Analysis of Supralingual, Oral and Intravenous Deliveries of Mycophenolic Acid

Gao

Tsai

et al. 2021

Pharmaceutics

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Mycophenolic acid (MPA) is commonly used for organ rejection prophylaxis via oral administration in the clinic. Recent studies have shown that MPA also has anticancer activities. To explore new therapeutic options for oral precancerous/cancerous lesions, MPA was designed to release topically on the dorsal tongue surface via a mucoadhesive patch. The objective of this study was to establish the pharmacokinetic (PK) and tongue tissue distribution of mucoadhesive MPA patch formulation after supralingual administration in rats and also compare the PK differences between oral, intravenous, and supralingual administration of MPA. Blood samples were collected from Sprague Dawley rats before and after a single intravenous bolus injection, a single oral dose, or a mucoadhesive patch administration on the dorsal tongue surface for 4 h, all with a dose of 0.5 mg/kg of MPA. Plots of MPA plasma concentration versus time were obtained. As multiple peaks were found in all three curves, the enterohepatic recycling (EHR) model in the Phoenix software was adapted to describe their PK parameters with an individual PK analysis method. The mean half-lives of intravenous and oral administrations were 10.5 h and 7.4 h, respectively. The estimated bioavailability after oral and supralingual administration was 72.4% and 7.6%, respectively. There was a 0.5 h lag-time presented after supralingual administration. The results suggest that the systemic plasma MPA concentrations were much lower in rats receiving supralingual administration compared to those receiving doses from the other two routes, and the amount of MPA accumulated in the tongue after patch application showed a sustained drug release pattern. Studies on the dynamic of drug retention in the tongue after supralingual administration showed that ~3.8% of the dose was accumulated inside of tongue right after the patch removal, ~0.11% of the dose remained after 20 h, and ~20.6% of MPA was not released from the patches 4 h after application. The data demonstrate that supralingual application of an MPA patch can deliver a high amount of drug at the site of administration with little systemic circulation exposure, hence lowering the potential gastrointestinal side effects associated with oral administration. Thus, supralingual administration is a potential alternative route for treating oral lesions.

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A comprehensive review on quantification of various biomarkers for the detection of oral carcinoma via electrochemical biosensors

Daweshar,

Pankaj,

Mewada

et al. 2024

Microchemical Journal

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Telomerase in saliva: An assistant marker for oral squamous cell carcinoma

Cited by 4 publications

References 13 publications

Comparing Length and Telomere Expression at Oral Precancerous and Cancerous Stages

Comparing Length and Telomere Expression at Oral Precancerous and Cancerous Stages

Pharmacokinetic Model Analysis of Supralingual, Oral and Intravenous Deliveries of Mycophenolic Acid

A comprehensive review on quantification of various biomarkers for the detection of oral carcinoma via electrochemical biosensors

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