Telomere Length Analysis and Preterm Infant Health: The Importance of Assay Design in the Search for Novel Biomarkers

Turner, Kara; Vasu, Vimal; Greenall, John; Griffin, Darren K.

doi:10.2217/bmm.14.13

Cited by 21 publications

(15 citation statements)

References 99 publications

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“…51 Benetos et al 52 showed that for a given chronological age, subjects with shorter telomere length are at increased risk for higher pulse wave velocity values.…”

Section: Discussionmentioning

confidence: 99%

Aortic Elastic Properties in Preschool Children Born Preterm

Komazec

Posod

Schwienbacher

et al. 2016

ATVB

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Objective-Preterm birth predisposes children to the development of cardiovascular diseases in adulthood. The aim of this study was to characterize elastic properties of the aorta at preschool age and test the hypothesis that prematurity is associated with decreased aortic distensibility and increased stiffness, both of which are predictors of increased cardiovascular risk. Approach and Results-In an observational study of 76 five-to seven-year-old children born at a gestational age <32 weeks and 79 term-born controls, elastic parameters of the ascending and descending abdominal aorta were determined noninvasively by means of M mode echocardiographic tracings and calculated using computerized wall contour analysis. Compared with children born at term, the preterm group showed significantly reduced distensibility and increased stiffness of the descending abdominal aorta. These results remained significant under multivariable adjustment for birth weight z score, maternal smoking in pregnancy, maternal education, family history of cardiovascular disease, breastfeeding, childhood nutrition, and current body mass index z score (multivariable odds ratios and 95% confidence intervals 5.1, 1.7-15.9; P=0.005 and 2.8, 1.0-7.9; P=0.046, respectively). Further adjustment for intravenous lipid therapy attenuated the strength of association. Elastic properties of the ascending aorta did not differ between the 2 study groups. Conclusions-Children

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“…51 Benetos et al 52 showed that for a given chronological age, subjects with shorter telomere length are at increased risk for higher pulse wave velocity values.…”

Section: Discussionmentioning

confidence: 99%

Aortic Elastic Properties in Preschool Children Born Preterm

Komazec

Posod

Schwienbacher

et al. 2016

ATVB

View full text Add to dashboard Cite

show abstract

“…salivary vs. leukocyte telomere length; neonates vs. children vs. adults, TRF vs. PCR-based measurements) many gaps remain in the current literature on the relation between preterm birth and telomere length. This was also emphasized by a recent review on telomere length and preterm birth [ 2 ].…”

Section: Introductionmentioning

confidence: 90%

“…gestational age <37 weeks) [ 1 ]. Next to respiratory morbidity and neurodevelopmental impairment, the ex-preterm infant phenotype is also characterized by adverse metabolic health in later life [ 2 ]: Already at a young adult age, ex-preterms have a higher risk for age-associated diseases, such as cardiovascular disease (CVD) [ 3 – 7 ] and have increased cardiovascular mortality [ 8 ]. The fetal origin hypothesis [ 9 ] states that this increased risk is programmed during fetal life and that adverse events during pregnancy lead to reprogramming, causing diseases in later adulthood.…”

Section: Introductionmentioning

confidence: 99%

Leukocyte Telomere Length in Young Adults Born Preterm: Support for Accelerated Biological Ageing

et al. 2015

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BackgroundSubjects born preterm have an increased risk for age-associated diseases, such as cardiovascular disease in later life, but the underlying causes are largely unknown. Shorter leukocyte telomere length (LTL), a marker of biological age, is associated with increased risk of cardiovascular disease.ObjectivesTo compare LTL between subjects born preterm and at term and to assess if LTL is associated with other putative cardiovascular risk factors at young adult age.MethodsWe measured mean LTL in 470 young adults. LTL was measured using a quantitative PCR assay and expressed as T/S ratio. We analyzed the influence of gestational age on LTL and compared LTL between subjects born preterm (n = 186) and at term (n = 284). Additionally, we analyzed the correlation between LTL and potential risk factors of cardiovascular disease.ResultsGestational age was positively associated with LTL (r = 0.11, p = 0.02). Subjects born preterm had shorter LTL (mean (SD) T/S ratio = 3.12 (0.44)) than subjects born at term (mean (SD) T/S ratio = 3.25 (0.46)), p = 0.003). The difference remained significant after adjustment for gender and size at birth (p = 0.001). There was no association of LTL with any one of the putative risk factors analyzed.ConclusionsYoung adults born preterm have shorter LTL than young adults born at term. Although we found no correlation between LTL and risk for CVD at this young adult age, this biological ageing indicator may contribute to CVD and other adult onset diseases at a later age in those born preterm.

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“…Several methods have been developed to assess telomere length. They include procedures which quantify the mean from total DNA extracted from a population of cells, and those which measure the telomere of individual chromosomes in a cell [ 8 ]. The most established method uses southern blot hybridization analysis of terminal restriction fragments (TRF) to measure the absolute telomere length and the subtelomeric regions.…”

Section: Introductionmentioning

confidence: 99%

Correlation of cord blood telomere length with birth weight

et al. 2017

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BackgroundIntrauterine growth restriction affects 3% of newborns; and the lightest 10% of whom are classified as small for gestational age (SGA). These low-birth weight newborns are at increased risk of neonatal morbidity such as hypoxia and hypoglycaemia. In later life, they are at higher risk of several age-related diseases such as cardiovascular and metabolic disorders and dementia. As having short telomeres is also associated with these diseases, we tested if these newborns might already start with shorter telomeres at birth.FindingsRelative telomere lengths were determined using quantitative real-time PCR in cord blood samples from 195 newborns of Chinese ancestry. Based on the telomere length normalised to a single copy gene and a reference DNA sample as internal control, we found statistically significant correlations between relative telomere length and both unadjusted and gestational age-adjusted birth weight, with the lighter newborns having shorter telomeres. The SGA birth weight group comprising the bottom 10% of the samples also had the shortest telomeres compared to the medium and heaviest birth weight groups.ConclusionsOur results indicate that there is reduction of cord blood telomere length for newborns with lower birth weight.

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Telomere Length Analysis and Preterm Infant Health: The Importance of Assay Design in the Search for Novel Biomarkers

Cited by 21 publications

References 99 publications

Aortic Elastic Properties in Preschool Children Born Preterm

Aortic Elastic Properties in Preschool Children Born Preterm

Leukocyte Telomere Length in Young Adults Born Preterm: Support for Accelerated Biological Ageing

Correlation of cord blood telomere length with birth weight

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