Telomeric motifs are present in a highly repetitive element in the Plasmodium berghei genome

Pace, Tomasino; Ponzi, Marta; Dore, E.; Frontali, C.

doi:10.1016/0166-6851(87)90106-x

Cited by 42 publications

(28 citation statements)

References 23 publications

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“…Además, se han caracterizado varias secuencias repetitivas subteloméricas no codificantes de P. falciparum como pRep20 (17-18) y PFCOL692 (19) entre otras, y un elemento de 2,3 Kb de Plasmodium berghei (20).…”

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Ubicación de la secuencia repetitiva PFCOL692 en fragmentos genómicos de Plasmodíum falciparum

Alvarado¹,

Rojas²,

Guerrero³

et al. 2000

biomedica

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ResumenLas secuencias repetitivas son componentes estructurales de los genomas eucariotes. Se desconoce la función de la mayoría de ellas, pero, al parecer, no son simplemente ADN egoísta sino que intervienen en procesos de recombinación y regulación génica. En este estudio se estableció la localización subtelomérica d e l a secuencia repetitiva PFCOL692 en los cromosomas de Plasmodium falciparum, a través de la caracterización de cuatro clones de la genoteca hEMBL4lPFCOL692, que contenían insertos entre 15 y 23Kb de ADN genómico del parásito; esta caracterización se hizo mediante análisis de restricción y la posible ubicación de PFCOL692 en el genoma se exploró utilizando sondas específicas para las regiones telomérica (pTB4.1) y subtelomérica (pRep20) de los cromosomas de P falciparum. El análisis de los mapas de restricción obtenidos permitió plantear una posible ubicación de PFCOL692 en el extremo de los cromosomas del parásito, donde las copias de esta secuencia se encuentran agrupadas en un segmento del subtelómero y con una posición ~0 n s e~a d a con respecto a la secuencia pRep20 Se sugiere, además, que PFCOL692 no se encuentra en el limite entre el subtelómero y el telómero.Palabras clave: Plasmodium falciparum, secuencias repetitivas, región subtelomérica Location o f the repetitive sequence PFCOL692 in genomic fragments of Plasmodium falciparum AbstractIn this paper we established the subtelomeric localization of the repetitive sequence PFCOL692 of Plasmodium falciparum by the characterization of four clones from a hEMBL41 PFCOL692 genomic library, which contains 15-23Kbp long inserts of parasite genomic DNA. Characterization was made by restriction analysis and the PFCOL692 localization in the genome was explored using specific probes for telomeric (pTB4.1) and subtelomeric regions (pRep20) of P falciparum chromosomes. We proposed a possible organization for the parasite chromosomes ends, where the copies of PFCOL692 are clusters in the subtelomeric region and their localization is conserved with respect to the pRep20 sequence. In addition, we established that PFCOL692 is not located next to the telomere.

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Ubicación de la secuencia repetitiva PFCOL692 en fragmentos genómicos de Plasmodíum falciparum

Alvarado¹,

Rojas²,

Guerrero³

et al. 2000

biomedica

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“…We subsequently cloned (in pUC8, using either HindlIlor BamHI-unique sites) the 2.3-kb unit (17) and showed it to be specific to P. berghei, in which it is present in several hundred copies, mostly in tandem organization. The basic 2.3-kb element contains a series of telomere-related motifs (17) and a bent DNA region (6).…”

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“…The basic 2.3-kb element contains a series of telomere-related motifs (17) and a bent DNA region (6). Furthermore, hybridization to molecular (PFGE) karyotypes indicated that the 2.3-kb * Corresponding author.…”

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confidence: 99%

“…repeat family is irregularly distributed over different chromosomes in a strain-specific fashion (17).…”

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confidence: 99%

“…Homology between subfragments d and 8, c and -y, b and , and a and a had been demonstrated by cross-hybridization (17), but in the case of the latter pair, hybridization between telomeric repeats in a and telomere-related motifs in a might have masked possible differences in the nonrepeated parts.…”

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Organization of subtelomeric repeats in Plasmodium berghei.

Dore¹,

Pace²,

Ponzi³

et al. 1990

Mol. Cell. Biol.

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Several (but not all) Plasmodium berghei chromosomes bear in the subtelomeric position a cluster of 2.3-kilobase (kb) tandem repeats. The 2.3-kb unit contains 160 base pairs of telomeric sequence. The resulting subtelomeric structure is one in which stretches of telomeric sequences are periodically spaced by a 2.1-kb reiterated sequence. This periodic organization of internal telomeric sequences might be related to chromosome-size polymorphisms involving the loss or addition of subtelomeric 2.3-kb units.

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The var genes of Plasmodium falciparum are located in the subtelomeric region of most chromosomes.

1996

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PfEMP1, a Plasmodium falciparum‐encoded protein on the surface of infected erythrocytes is a ligand that mediates binding to receptors on endothelial cells. The PfEMP1 protein, which is encoded by the large var gene family, shows antigenic variation and changes in binding phenotype associated with alterations in antigenicity. We have constructed a yeast artificial chromosome contig of chromosome 12 from P. falciparum and show that var genes are arranged in four clusters; two lie amongst repetitive subtelomeric sequences and two occur in the more conserved central region. Analysis of parasite chromosomes by pulsed field gel electrophoresis (PFGE) demonstrates that most contain var genes and two‐dimensional PFGE has shown that var genes are located at chromosome ends interspersed amongst repetitive sequences present in the subtelomeric complex. Analysis of a var gene located in the subtelomeric region of chromosome 12 has shown that it has close homologues at the opposite end of the chromosome and in the subtelomeric region of two other chromosomes. This suggests that recombination between heterologous chromosomes has occurred in the subtelomeric regions of these chromosomes. The subtelomeric location of var genes dispersed amongst repetitive sequences has important implications for generation of antigenic variants and novel cytoadherent specificities of this protein.

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Telomeric motifs are present in a highly repetitive element in the Plasmodium berghei genome

Cited by 42 publications

References 23 publications

Ubicación de la secuencia repetitiva PFCOL692 en fragmentos genómicos de Plasmodíum falciparum

Ubicación de la secuencia repetitiva PFCOL692 en fragmentos genómicos de Plasmodíum falciparum

Organization of subtelomeric repeats in Plasmodium berghei.

The var genes of Plasmodium falciparum are located in the subtelomeric region of most chromosomes.

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