Abstract:Many proteins produced in E. coli accumulate in inclusion bodies. This study aims to detect the role of temperature in reducing the formation of inclusion bodies during recombinant human prethrombin-2expressed in E. coli BL21 (DE3) Arctic Express host. In this study, we created a host growth curve to find out the right time to add an inducer. The inducer used in our experiment was IPTG 0.1 mM. The fermentation process use a temperature of 12°C and 22°C. The results showed that recombinant human prethrombin-2 w… Show more
“…1. Other reports [42][43][44][45][46] are present on the cloning and expression of xylosidase gene in E. coli from various sources but till now no report is present on the cloning and expression of xylosidase gene from C. clariavum.…”
The present study describes the cloning, expression, purification and characterization of a xylosidase gene from Clostridium clariflavum into E. coli BL21 (DE3) using the expression vector pET-21a(+) for utilization in biofuel production.
“…1. Other reports [42][43][44][45][46] are present on the cloning and expression of xylosidase gene in E. coli from various sources but till now no report is present on the cloning and expression of xylosidase gene from C. clariavum.…”
The present study describes the cloning, expression, purification and characterization of a xylosidase gene from Clostridium clariflavum into E. coli BL21 (DE3) using the expression vector pET-21a(+) for utilization in biofuel production.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.