Temperature studies and recent advances with fish cells in vitro

Bols, Niels C.; Mosser, Dick D.; Steels, G.B.

doi:10.1016/0300-9629(92)90235-i

Cited by 79 publications

(51 citation statements)

References 57 publications

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“…The temperatures at which VHSV survived are physiological (4-26°C) for coldwater fish (Bols et al 1992) and similar to what has been reported by others for VHSV in suspension. For example, VHSV survived in freshwater for 5 or more days at 25°C, increasing to more than 25 days at 4°C.…”

Section: Inactivation Of Adsorbed Virus With Time Temperature and Drsupporting

confidence: 89%

Using 96-well tissue culture polystyrene plates and a fluorescence plate reader as tools to study the survival and inactivation of viruses on surfaces

2011

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A method for studying the behavior of viruses on surfaces has been developed and is illustrated by determining the temperatures that inactivate adsorbed viral hemorrhagic septicemia virus (VHSV) and the concentration of 1-propanol that disinfected surfaces with adsorbed VHSV and chum salmon virus (CSV). VHSV is a rhabdovirus; CSV, a reovirus, and they were detected with two fish cell lines, EPC and CHSE-214, respectively. When polystyrene tissue culture surfaces were incubated with virus, rinsed, and left to dry, they still supported the attachment and spreading of cell lines and after 7 days these cells showed the characteristic CPE of the viruses. Thus cells appeared to be infected directly from surfaces on which viruses had been adsorbed. Applying this property to 96-well plates allowed duplicate surfaces to be examined for their infectiousness or support of CPE. For each treatment 80 replicate surfaces in a 96-well plate were tested at one time and the results expressed as the number of wells showing CPE. VHSV adsorbed to polystyrene was inactivated by drying in the dark at temperatures above 14°C, but remained infectious for at least 15 days of drying at 4°C. For chemical sterilization of polystyrene surfaces with adsorbed virus, disinfection was achieved with 1-propanol at 40% for VHSV and at 60% for CSV. As CPE can be conveniently monitored in 96-well plates with a fluorescence plate reader, this method can be used to rapidly evaluate a variety of treatments for their ability to inactivate surface-bound viruses.

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Section: Inactivation Of Adsorbed Virus With Time Temperature and Drsupporting

confidence: 89%

Using 96-well tissue culture polystyrene plates and a fluorescence plate reader as tools to study the survival and inactivation of viruses on surfaces

2011

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“…It is known that a moderate heat stress terminates or delays mammalian cell growth, whereas a severe heat stress is lethal (Kü hl and Rensing 2000). In a similar manner, cells from poikilothermic teleosts are not able to grow at their sublethal temperatures (Hightower and Renfro 1988;Bols et al 1992). For example, the rainbow trout, Oncorhynchus mykiss, cell line RTG-2 cannot grow at 26-28ЊC and cannot survive at 30ЊC (Mosser et al 1986).…”

Section: Introductionmentioning

confidence: 99%

Characterization of goldfish heat shock protein–30 induced upon severe heat shock in cultured cells

Kondo

Harano²,

Nakaya³

et al. 2004

Cell Stress Chaper

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Temperature-dependent changes of growth rate and protein components were investigated for primary cultured cells derived from goldfish caudal fin. When the culture temperature was shifted from 20ЊC to 35ЊC and 40ЊC, the growth rate was increased at 35ЊC as compared with that at 20ЊC, but no cell growth was observed at 40ЊC. The differential scanning calorimetry demonstrated the onset of the endothermic reaction for goldfish cellular components at 40ЊC. Therefore, the temperature shift to 40ЊC was found to be of severe heat shock for goldfish cultured cells. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis revealed that, although expression of 70-kDa components was slightly induced at 35ЊC, the temperature shift to 40ЊC markedly induced the expression of the 30-kDa component in addition to that of 70-kDa component. The N-terminal amino acid sequencing identified the 30-and 70-kDa components to be heat shock protein (Hsp)-30 and Hsp70, respectively. Northern blot analysis revealed that the enhanced Hsp30 messenger ribonucleic acid (mRNA) levels were only observed at 40ЊC, whereas Hsp70 mRNA was slightly accumulated at 35ЊC. These results indicated that Hsp30 might have important functions under severe heat stress condition.

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“…Moreover, there is a striking relationship between the induction temperature and the organism's environment (Lindquist, 1986;Lindquist and Craig, 1988;Maio, 1995). Bols et al (1992) reviewed the use of fish cell cultures to study three temperature phenomena, including hsp synthesis, temperature acclimation and heat resistance. In the RTG-2 cell line from rainbow trout, a cause and effect relationship between the synthesis of the major hsps and the development of thermotolerance is suggested (Mosser and Bols, 1988).…”

Section: Mentsmentioning

confidence: 99%

In Vitro Thermal Effects on Embryonic Cells of Endangered Hawksbill TurtleEretmochelys imbricata

et al. 2013

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The hawksbill turtle is an ectotherm, whose sex is determined by temperature during embryonic development. This study aimed to determine whether embryonic hawksbill turtle cells respond differently to temperature than mammalian cells. Embryonic hawksbill turtle cells were established in culture, and thermal effects on these cells were investigated in vitro. Cells were maintained in Dulbecco's Modified Eagle Medium supplemented with non-essential amino acids, vitamin solution, sodium pyruvate, and 10% fetal bovine serum at 33°C and cell proliferation occurred at 25-33°C. When cells were incubated at 37°C (the temperature of mammalian cell culture) for 24 h, cell growth was completely inhibited. This growth inhibition was evidently recovered by changing the incubation temperature back to 33°C. Expression of heat shock protein was found to increase with elevating culture temperature from 25 to 33°C.

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Temperature studies and recent advances with fish cells in vitro

Cited by 79 publications

References 57 publications

Using 96-well tissue culture polystyrene plates and a fluorescence plate reader as tools to study the survival and inactivation of viruses on surfaces

Using 96-well tissue culture polystyrene plates and a fluorescence plate reader as tools to study the survival and inactivation of viruses on surfaces

Characterization of goldfish heat shock protein–30 induced upon severe heat shock in cultured cells

In Vitro Thermal Effects on Embryonic Cells of Endangered Hawksbill TurtleEretmochelys imbricata

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