Temperature-Triggered Self-Assembly of Elastin-Like Block Co-Recombinamers:The Controlled Formation of Micelles and Vesicles in an Aqueous Medium

Martin, Laura B.; Castro, Emilio; Ribeiro, Artur; Alonso, Matilde; Rodríguez-Cabello, J Carlos

doi:10.1021/bm201436y

Cited by 91 publications

(109 citation statements)

References 39 publications

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“…In recent years, a great deal of stimuli-responsive amphipathic polymer micelles that respond to internal or external stimuli including pH, [1,2] temperature, [3] redox, [4] light, [5], and magnetic field [6] have been abundantly investigated due to their broad range of potential applications as nanocarriers, [7] biosensors, [8,9], and biochemical gates. [10] Among the various stimuli, light is one of the most attractive external stimuli because of the superiority in spatial and temporal control.…”

Section: Introductionmentioning

confidence: 99%

Self-assembly and morphology transition of amphipathic spiropyran-based random copolymers to control drug release

Chen

Liu

et al. 2016

Designed Monomers and Polymers

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An amphipathic spiropyran-based random copolymer P(SPMA-co-DMAEMA) was synthesized by atom transfer radical polymerization, and the resulting copolymer was characterized by means of 1 H nuclear magnetic resonance spectroscopy, Fourier transform infrared spectroscopy, and gel permeation chromatography. The self-assembly behaviors and morphology transition were systematically investigated under single and combined external environmental stimuli by transmission electron microscopy. With coumarin 102 as the model drug molecule, the selfassembly micelles were used to control drug loading, release, and re-encapsulation to some extent. The characterization results indicated the successful preparation of the spiropyran-based random copolymer P(SPMA-co-DMAEMA). The external stimuli had some influences on the morphology of the self-assembly aggregate, and the 'schizophrenic' behavior was interestingly found in this work. The drug release experiments showed the reversible loading and release process up to a point, which might expand the potential application domain of the amphiphilic spiropyran-based random copolymer in drug delivery.

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Section: Introductionmentioning

confidence: 99%

Self-assembly and morphology transition of amphipathic spiropyran-based random copolymers to control drug release

Chen

Liu

et al. 2016

Designed Monomers and Polymers

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“…Smaller self-assembling oligopeptides can be produced recombinantly (9), but the small sizes of these peptides preclude the incorporation of biologically relevant motifs. Elastin-like polypeptides (10) have been assembled into various structures, including micelles (11) and vesicles (12), although the direct visualization of a bilayer membrane or vesicular encapsulation has not been explicitly shown (12).…”

mentioning

confidence: 99%

Self-assembly of tunable protein suprastructures from recombinant oleosin

Vargo

Parthasarathy

Hammer

2012

Proc. Natl. Acad. Sci. U.S.A.

104

130

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Using recombinant amphiphilic proteins to self-assemble suprastructures would allow precise control over surfactant chemistry and the facile incorporation of biological functionality. We used cryo-TEM to confirm self-assembled structures from recombinantly produced mutants of the naturally occurring sunflower protein, oleosin. We studied the phase behavior of protein self-assembly as a function of solution ionic strength and protein hydrophilic fraction, observing nanometric fibers, sheets, and vesicles. Vesicle membrane thickness correlated with increasing hydrophilic fraction for a fixed hydrophobic domain length. The existence of a bilayer membrane was corroborated in giant vesicles through the localized encapsulation of hydrophobic Nile red and hydrophilic calcein. Circular dichroism revealed that changes in nanostructural morphology in this family of mutants was unrelated to changes in secondary structure. Ultimately, we envision the use of recombinant techniques to introduce novel functionality into these materials for biological applications.protein surfactants | self-assembled suprastructure | cryogenic transmission microscopy

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“…[54][55][56][57][58] This selfassembly behavior can be induced by the design of the ELR construct itself, conjugation of a drug, addition of charged molecules or mediated by an external stimulus, such as temperature, pH, etc.…”

Section: Self-assembled Elrs For Nano-and Microparticle Synthesismentioning

confidence: 99%

Nanotechnological Approaches to Therapeutic Delivery Using Elastin-Like Recombinamers

et al. 2015

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This review discusses the use of elastin-like polymers and their recombinant version, elastinlike recombinamers, in drug-delivery systems. These macromolecules exhibit a number of interesting properties that are rarely found together in any other family of materials, especially extremely high biocompatibility, high bioactivity and functionality, complex yet fully controlled composition and stimuli responsiveness.Appropriate design of these molecules opens up a broad range of different possibilities for their use in new therapeutic platforms. The first of these described herein is the use of ELRs in singlemolecule devices as therapeutic entities on their own. Subsequently, we describe how the selfassembly properties of these materials can be exploited to create nanocarriers and, eventually, microcarriers that are able to temporally and spatially control and direct the release of their drug load. Intracellular drug-delivery devices and nanocarriers for treating cancer are among the uses described in that section. Finally, the use of ELRs as base materials for implantable drug depots, in the form of hydrogels, is discussed.

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Temperature-Triggered Self-Assembly of Elastin-Like Block Co-Recombinamers:The Controlled Formation of Micelles and Vesicles in an Aqueous Medium

Cited by 91 publications

References 39 publications

Self-assembly and morphology transition of amphipathic spiropyran-based random copolymers to control drug release

Self-assembly and morphology transition of amphipathic spiropyran-based random copolymers to control drug release

Self-assembly of tunable protein suprastructures from recombinant oleosin

Nanotechnological Approaches to Therapeutic Delivery Using Elastin-Like Recombinamers

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