Temporal and Spatial Variation of the Skin-Associated Bacteria from Healthy Participants and Atopic Dermatitis Patients

Barnes, Christopher J.; Clausen, Maja‐Lisa; Asplund, Maria E.; Rasmussen, L.; Olesen, Caroline Meyer; Yüsel, Yasemin Topal; Andersen, Paal Skytt; Litman, Thomas; Hansen, Anders J.; Agner, Tove

doi:10.1128/msphere.00917-21

Cited by 6 publications

(11 citation statements)

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“…Nineteen infants with AD (mean age at inclusion (range): 12.5 months (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22); female: 31.6%; mean O-SCORAD (range): 19.0 (8.0-31.9)) and 19 healthy infants (mean age at inclusion (range): 8.5 months (3-21); female: 57.9%) were included in the study (Table SI). Patients were allowed to use topical treatments (i.e.…”

Section: Resultsmentioning

confidence: 99%

“…As part of Barnes et al ( 19 ) study, 20 adults with AD and 20 healthy controls were sampled repeatedly at the same clinic during the same period of time, using tape-stripping. To investigate the similarities between infants and adults, the mean composition for each skin group was calculated at different age ranges (0–6, 7–12, 13–18, 18+ months, and adults) and compared at the family level ( Fig.…”

Section: Resultsmentioning

confidence: 99%

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Altered Maturation of the Skin Microbiome in Infants with Atopic Dermatitis

et al. 2022

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The aim of this study was to investigate the early-life development of the skin microbiome in atopic dermatitis. Nineteen infants with atopic dermatitis and 19 healthy infants were evaluated 3 times, at 3 months intervals, within the first 30 months of life. Tape-strips were collected from volar forearms, cheeks, and eczema lesions, and the skin microbiome was assessed by 16S rRNA sequencing. Both the community structure and richness of the skin microbiome of infants with atopic dermatitis differed significantly from that of healthy infants, with greater richness in healthy infants. For infants with atopic dermatitis, the community composition was not dominated by Staphylococci. For healthy infants, community composition and richness correlated significantly with age, while such a pattern was not revealed in subjects with atopic dermatitis. This suggests a slower maturation of the skin bacterial communities in atopic dermatitis, which precedes the staphylococcal predominance observed in older children and adults.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Altered Maturation of the Skin Microbiome in Infants with Atopic Dermatitis

et al. 2022

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“…Tape stripping is an emerging, non-invasive, and easily applicable skin sampling technique, which has been used to study mRNA [ 6 ], proteins [ 7 ], lipids [ 8 ], and microbiome [ 9 ] on the skin surface, especially in pediatric patients [ 6 , 24 ].…”

Section: Discussionmentioning

confidence: 99%

“…Tape stripping is such a non-invasive skin sampling technique that applies adhesive tapes to obtain molecular information on mRNA [ 6 ], proteins [ 7 ], lipids [ 8 ], and microbiome [ 9 ] from the skin. Because the technique is easily applicable, it is particularly useful for sampling large patient cohorts and for pediatric patients, from whom an epidermal transcriptomic profile may lead to discovery of biomarkers [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Profiling the Atopic Dermatitis Epidermal Transcriptome by Tape Stripping and BRB-seq

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Andersen

et al. 2022

IJMS

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Tape stripping is a non-invasive skin sampling technique, which has recently gained use for the study of the transcriptome of atopic dermatitis (AD), a common inflammatory skin disorder characterized by a defective epidermal barrier and perturbated immune response. Here, we performed BRB-seq—a low cost, multiplex-based, transcriptomic profiling technique—on tape-stripped skin from 30 AD patients and 30 healthy controls to evaluate the methods’ ability to assess the epidermal AD transcriptome. An AD signature consisting of 91 differentially expressed genes, specific for skin barrier and inflammatory response, was identified. The gene expression in the outermost layers, stratum corneum and stratum granulosum, of the skin showed highest correlation between tape-stripped skin and matched full-thickness punch biopsies. However, we observed that low and highly variable transcript counts, probably due to low RNA yield and RNA degradation in the tape-stripped skin samples, were a limiting factor for epidermal transcriptome profiling as compared to punch biopsies. We conclude that deep BRB-seq of tape-stripped skin is needed to counteract large between-sample RNA yield variation and highly zero-inflated data in order to apply this protocol for population-wide screening of the epidermal transcriptome in inflammatory skin diseases.

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“…Finally, comparing skin‐associated bacterial communities of adult healthy volunteers with lesional and nonlesional skin of adult AD patients, the bacterial community was found to vary markedly according to AD severity, and between patients with or without filaggrin mutations, but no significant temporal variation was found within either AD patients or healthy controls 22 …”

Section: Temporal Variations Of Atopic Dermatitis In Children and Adultsmentioning

confidence: 97%

Skin microbiome dysbiosis and the role of Staphylococcus aureus in atopic dermatitis in adults and children: A narrative review

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Connetable

Kérob

et al. 2023

Acad Dermatol Venereol

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A dysfunctional epidermal barrier, which may be associated with mutations in the filaggrin gene in genetically predisposed individuals or harmful effects of environmental agents and allergens, contributes to the development of atopic dermatitis (AD) due to an interplay between the epithelial barrier, immune defence and the cutaneous microbiome. The skin of patients with AD is frequently over‐colonized by biofilm‐growing Staphylococcus aureus, especially during flares, causing dysbiosis of the cutaneous microbiota and a decrease in bacterial diversity that inversely correlates with AD severity. Specific changes in the skin microbiome can be present before clinical AD onset in infancy. Additionally, local skin anatomy, lipid content, pH, water activity and sebum secretion differ between children and adults and generally correlate with the predominant microbiota. Considering the importance of S. aureus in AD, treatments aimed at reducing over‐colonization to rebalance microbial diversity may help manage AD and reduce flares. Anti‐staphylococcal interventions in AD will contribute to a decrease in S. aureus superantigens and proteases that cause damage and inflammation of the skin barrier while concomitantly increasing the proportion of commensal bacteria that secrete antimicrobial molecules that protect healthy skin from invading pathogens. This review summarizes the latest data on targeting skin microbiome dysbiosis and S. aureus over‐colonization to treat AD in adults and children. Indirect AD therapies, including emollients ‘plus’, anti‐inflammatory topicals and monoclonal antibodies, may have an impact on S. aureus and help control bacterial diversity. Direct therapies, including antibacterial treatments (antiseptics/topical or systemic antibiotics), and innovative treatments specifically targeting S. aureus (e.g. anti‐S. aureus endolysin, and autologous bacteriotherapy), may be effective alternatives to mitigate against an increase in microbial resistance and allow a proportionate increase in the commensal microbiota.

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Temporal and Spatial Variation of the Skin-Associated Bacteria from Healthy Participants and Atopic Dermatitis Patients

Abstract: The bacteria associated with human skin may influence skin barrier function and the immune response. Previous studies have attempted to understand the factors that regulate the skin bacteria, characterizing the spatial-temporal variation of the skin bacteria within unaffected skin.

Cited by 6 publications

References 44 publications

Altered Maturation of the Skin Microbiome in Infants with Atopic Dermatitis

Altered Maturation of the Skin Microbiome in Infants with Atopic Dermatitis

Profiling the Atopic Dermatitis Epidermal Transcriptome by Tape Stripping and BRB-seq

Skin microbiome dysbiosis and the role of Staphylococcus aureus in atopic dermatitis in adults and children: A narrative review

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