Temporal and spatial visualization of water uptake into human hair studied by infrared microscopy

Fuse, Naoya; Matsui, Masashi; Matsue, Yukako

doi:10.1007/s44211-022-00074-x

Cited by 7 publications

(8 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…IR spectroscopy has also been shown to be a useful technique for studying aspects of the structure of hair. As an example, it has been used to show variation of the internal structure in human hair such as in the localization of amide bonds, C-H bonds and sulphonic acid groups formed by bleaching the hair [11,12]. Also, again using IR spectroscopy, the secondary structural composition of a protein can be determined by deconvolution of the amide I band and, using this, it was found that αhelix in mechanically compressed hair converts to βsheet [13].…”

Section: Discussionmentioning

confidence: 99%

“…Hair samples were obtained from 10 subjects of each age group with the hair fibres being cut at 1 cm from the root region. The method of Fuse et al [12] was used to cut smooth sections of 6.0 μm lengths from the fibres using Leica RM2265, a rotary microtome (Leica Biosystems, Tokyo, Japan). These sections were then used to determine the free amine group content of the hair by reaction of amine groups in the hair with NBD-Cl and then measurement of the fluorescence intensity across the hair crosssection according to the method reported by Masaki et al [15] as follows.…”

Section: Visualization and Quantification Of Amino Groups In The Hairmentioning

confidence: 99%

“…A FT-IR microscope (iN-10, Thermo Scientific, Waltham, USA) was used to visualize distribution of protein, as described in reference [12]. In order to visualize distribution of protein, the hairs were sliced to a thickness of 6.0 μm using a microtome.…”

Section: Ft-ir Microscopy Analysismentioning

confidence: 99%

See 2 more Smart Citations

Age‐related hair denaturation related to protein carbonyls

Fuse,

Morita,

Matsue

2023

Intern J of Cosmetic Sci

Self Cite

View full text Add to dashboard Cite

ObjectiveHair ageing is accompanied by hair fibres becoming irregularly shaped causing them to stick out in irregular directions or have more curliness and being spread out. This is believed to be due to changes within the hair fibre structure which occur with ageing, and one of the causes of these changes could be an increase in the number of protein carbonyl groups present in the hair. The aim of this study is to investigate the internal denaturation of hair related to protein carbonyls in attempt to gain new insight into age‐related changes that occur in hair.MethodsThe degree of carbonylation of the hair structural protein as determined by fluorescent labelling and Western blotting analysis was used to investigate the primary structure of hair protein. The amount of helix, a common conformation in the secondary structure of proteins, in hair in groups of women with different ages was also analysed using infrared microscopy coupled with multivariate curve resolution (MCR). From the results of this, an image of the two‐dimensional distribution of the α‐helices was generated for the hair taken from each age group. Also, high‐pressure differential scanning calorimetry (HPDSC) of the hair in water was performed on the hair taken from each age group to determine the peak temperature of endothermic effect and the enthalpy of denaturation.ResultsWe found that the amino group content in hair proteins decreased and Type II keratin, one of the subunits of intermediate filament, was more carbonylated with age. The results of the MCR indicated eight separate components, including components of the secondary structure of proteins, such as α helices and β sheets. Two‐dimensional images of the hair cross‐sections revealed that the presence of α helices decreased with age. In addition, data from the HPDSC showed that the enthalpy associated with the denaturing temperature also significantly decreased with age.ConclusionThese results suggest that there is a negative correlation between age and structural integrity of the helix segment in intermediate filament. The results of this study also show that there is a positive correlation between age‐related hair denaturation and protein carbonyls.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Visualization and Quantification Of Amino Groups In The Hairmentioning

confidence: 99%

See 1 more Smart Citation

Age‐related hair denaturation related to protein carbonyls

Fuse,

Morita,

Matsue

2023

Intern J of Cosmetic Sci

Self Cite

View full text Add to dashboard Cite

show abstract

“…The FT-IR microscope technique is also often employed for thin hair cross sections to visualize the distribution of additives. Fuse et al have visualized the water uptake in human hair by using heavy water (D 2 O) instead of lightweight water (H 2 O) 27,40) . Penetration of this heavy water from the cuticle to medulla was quantified by monitoring the O-D stretching band located at 2500 cm −1 .…”

Section: Visualization and Controlled Delivery Of Additivesmentioning

confidence: 99%

“…The second key point for developing better hair care products is the controlled delivery of active ingredients. Up to now, their distribution has been investigated by FT-IR microscope 26,27,40) , laser confocal microscopy 41−44) , and scanning electron microscopy (SEM) 45,46) . Moreover, an attempt to apply nanoscale secondary ion mass spectrometry (NanoSIMS) technology in hair research has been recently successful 47−50) .…”

mentioning

confidence: 99%

Recent Progress in Hair Science and Trichology

Uyama

2024

J. Oleo Sci.

View full text Add to dashboard Cite

IntroductionHuman scalp hair has a lot of important functions. Seasonal variations in hair growth and goosebumps caused by cold stimuli or emotions point to its evolutionary history 1,2) . The main function of hair is to protect our head and neck from sunlight (mainly UV rays) , thermal pain (extreme heat/cold) , and physical damage 3,4) . Hair is also essential as an excretory system for trace metals and acts as an accumulating tissue. Thus, the metal content in hair can reflect the body s condition for a long period, recording the history of personal exposure to metals 5−8) . Last but not least, hair style is deeply tied to gender 9−12) , religion, and culture, as we arrange our hair as we want to complete our entire look. It is said that the first barbering services were performed in ancient Egypt in 5000 B.C. with tools made from oyster shells or sharpened flint. In ancient Egyptian culture, barbers were highly respected individuals and surgeons were said to also serve as barbers. In addition to this, the earliest records on the usage of henna as a hair dye go back to ancient Egypt, where people used henna to decorate and color their hair and nails 13) . In Japan, people

show abstract

Analysis of the hydration water on the surface of human hair using a combination of infrared absorption vibrational spectroscopy and multivariate curve resolution

Chikami,

Maeda,

Latag

et al. 2024

Surface & Interface Analysis

View full text Add to dashboard Cite

Modern society's keen regard for aesthetics made hair products an integral part of a multi‐billion‐dollar cosmetic industry. Hair care products (e.g., shampoos and conditioners) and chemical treatments (e.g., bleaching and permanent waving) result in various effects on the morphological attributes of hair. Generally, water adsorbed on the hair surface is known to significantly dictate the hair's mechanical characteristics (smoothness and friction), and hair's macroscopic wettability has been commonly used to indicate its surface properties. However, an approach to selectively characterize the hydration water in the hair surface is required to accurately understand the intermolecular events between the hair and its vicinal water. In this paper, we successfully obtained the infrared (IR) absorption spectra of the hydration water of human hair. We employed the multivariate curve resolution‐alternating least square (MCR‐ALS) method to separate the hydration and bulk water spectra from the whole spectra. Comparing the IR spectra of the hydration water of chemically untreated and bleached hair samples, we conclude that water molecules form strong hydrogen bonds with the bleached hair surface due to the destruction of the hair's hydrophobic outer layer and the consequent formation of hydrophilic residues.

show abstract

Temporal and spatial visualization of water uptake into human hair studied by infrared microscopy

Cited by 7 publications

References 10 publications

Age‐related hair denaturation related to protein carbonyls

Age‐related hair denaturation related to protein carbonyls

Recent Progress in Hair Science and Trichology

Analysis of the hydration water on the surface of human hair using a combination of infrared absorption vibrational spectroscopy and multivariate curve resolution

Contact Info

Product

Resources

About