Temporal changes in liver tissue metabolome of lambs fed low‐quality roughage

Abstract

Early experience with low-quality roughage may induce adaptations in ruminants' metabolism. This study was conducted to explore the variation in the hepatic metabolomes of lambs fed low-quality roughage beginning early in life. Five lambs were fed Sudan grass hay (crude protein (CP): 4.6% of the dry matter (DM), neutral detergent fiber, 68.5% of DM) for 6 months during time periods P1, P2 and P3, which consisted of 2 months each. The metabolizable energy (ME) and CP intake satisfied lambs' maintenance requirements in P1 and P2, but the ME intake was 78.5% of the maintenance ME requirement in P3. Liver metabolomics was carried out in P2 and P3 by the capillary electrophoresis and time-of-flight mass spectrometry system. Eight amino acids and six amino acid metabolism-related metabolites were altered between P2 and P3. Several intermediates of glycolysis/gluconeogenesis decreased, while nicotinamide adenine dinucleotide and nicotinamide adenine dinucleotide phosphate increased in P3. Taurocholic acid and taurine increased, while glycocholic acid decreased in P3. The results suggested that amino acid utilization and the efficiency of glycolysis/gluconeogenesis might be adjusted to accommodate the low-quality roughage fed to the lambs during early stages of life. The composition of bile acids might also be optimized to promote the efficiency of lipid absorption.

Recent advances in capillary electrophoresis‐mass spectrometry: Instrumentation, methodology and applications

“…[88,112,118] TOF Untargeted biomarker discovery (metabolites). Identification of 499 [112], 286 [88], 60 [92] and 300 [118] [88,92,112,118] Rat urine [225] Human urine [226] Corynebacterium glutamicum [157] Mouse serum/plasma [144,145,150], feces [145], luminal [145], arterial [147] and colonic [144], muscle [139] and brain [143] tissue Sheep liver tissue [230] Pork [231] Renal carcinoma cells [232] CZE(N) HMT sheath liquid [234] TOF Identification of 106 [146], 412 [159] and 367 [161] metabolites Influence of taurine on carbohydrate metabolism [234] Tobacco leaves [159,161] CZE(R) FS 50 mM ammonium acetate…”

Metabolomics Integrated with Transcriptomics Reveals a Subtle Liver Metabolic Risk in Dairy Cows Fed Different Crop By‐products

“…To our knowledge, no study has been reported about liver transcriptomics from dairy cows fed different sources of crop byproducts. However, the liver transcriptomics in terms of cows' feed efficiency has been well documented in several studies, [37,39] and the notable consistent results were that lower feed efficiency cows presented upregulated lipid metabolism and inflammation. In ruminants, the lipid metabolism occurs in adipose tissues as well as in liver tissue, with limited metabolic capacity.…”