Temporal physiological, transcriptomic and metabolomic analyses revealed molecular mechanism of<i>Canna indica</i>’s response to Cr stress

Wei, Zhao; Chen, Zhongbing; Yang, Xiaomin; Luying, Sheng; Huan, Mao; Zhu, Shuijin

doi:10.1101/2023.01.14.524062

Cited by 4 publications

(3 citation statements)

References 60 publications

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“…In contrast, ROS levels were measured by a multifunctional ELISA (Berten, USA) for 10 min, with an excitation wavelength of 499 nm and an emission wavelength of 521 nm. The absorbance measured can be converted into the content according to the formula given by the kit (Wei et al, 2023b).…”

Section: Antioxidant Enzyme Content Gsh Content Ros and Mda Levelsmentioning

confidence: 99%

Recruitment and metabolomics between Canna indica and rhizosphere bacteria under Cr stress

Mao,

Zhao,

Yang

et al. 2023

Front. Microbiol.

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It is of positive significance to explore the mechanism of antioxidant and metabolic response of Canna indica under Cr stress mediated by rhizosphere niche. However, the mechanisms of recruitment and interaction of rhizosphere microorganisms in plants still need to be fully understood. This study combined physiology, microbiology, and metabolomics, revealing the interaction between C. indica and rhizosphere microorganisms under Cr stress. The results showed that Cr stress increased the content of malondialdehyde (MDA) and oxygen-free radicals (ROS) in plants. At the same time, the activities of antioxidant enzymes (SOD, POD, and APX) and the contents of glutathione (GSH) and soluble sugar were increased. In addition, Cr stress decreased the α diversity index of C. indica rhizosphere bacterial community and changed its community structure. The dominant bacteria, namely, Actinobacteriota, Proteobacteria, and Chloroflexi accounted for 75.16% of the total sequence. At the same time, with the extension of stress time, the colonization amount of rhizosphere-dominant bacteria increased significantly, and the metabolites secreted by roots were associated with the formation characteristics of Proteobacteria, Actinobacteria, Bacteroidetes, and other specific bacteria. Five critical metabolic pathways were identified by metabolome analysis, involving 79 differentially expressed metabolites, which were divided into 15 categories, mainly including lipids, terpenoids, and flavonoids. In conclusion, this study revealed the recruitment and interaction response mechanism between C. indica and rhizosphere bacteria under Cr stress through multi-omics methods, providing the theoretical basis for the remediation of Cr-contaminated soil.

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Section: Antioxidant Enzyme Content Gsh Content Ros and Mda Levelsmentioning

confidence: 99%

Recruitment and metabolomics between Canna indica and rhizosphere bacteria under Cr stress

Mao,

Zhao,

Yang

et al. 2023

Front. Microbiol.

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“…Recently, high-throughput omics technologies, including transcriptomics, proteomics, and metabolomics, have been widely applied to study the responses of hyperaccumulators, such as Phytolacca americana (Zhao et al, 2011), S. nigrum (Wang et al, 2022), S. alfredii (Wu et al, 2020), and Brassica napus (Zhang et al, 2019), to heavy metal stress. These omics technologies have further expanded our understanding of the complex biological processes induced by Cd stress (Wang et al, 2022;Wei et al, 2023). Therefore, in the present study, we employed a combination of physiological, transcriptomic, and metabolomic analyses to investigate how A. manihot acclimates to Cd stress.…”

Section: Introductionmentioning

confidence: 99%

Integrative physiological, transcriptomic, and metabolomic analysis of Abelmoschus manihot in response to Cd toxicity

Wu,

Xu,

Tang

et al. 2024

Front. Plant Sci.

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Rapid industrialization and urbanization have caused severe soil contamination with cadmium (Cd) necessitating effective remediation strategies. Phytoremediation is a widely adopted technology for remediating Cd-contaminated soil. Previous studies have shown that Abelmoschus manihot has a high Cd accumulation capacity and tolerance indicating its potential for Cd soil remediation. However, the mechanisms underlying its response to Cd stress remain unclear. In this study, physiological, transcriptomic, and metabolomic analyses were conducted to explore the response of A. manihot roots to Cd stress at different time points. The results revealed that Cd stress significantly increased malondialdehyde (MDA) levels in A. manihot, which simultaneously activated its antioxidant defense system, enhancing the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) by 19.73%–50%, 22.87%–38.89%, and 32.31%–45.40% at 12 h, 36 h, 72 h, and 7 days, respectively, compared with those in the control (CK). Moreover, transcriptomic and metabolomic analyses revealed 245, 5,708, 9,834, and 2,323 differentially expressed genes (DEGs), along with 66, 62, 156, and 90 differentially expressed metabolites (DEMs) at 12 h, 36 h, 72 h, and 7 days, respectively. Through weighted gene coexpression network analysis (WGCNA) of physiological indicators and transcript expression, eight hub genes involved in phenylpropanoid biosynthesis, signal transduction, and metal transport were identified. In addition, integrative analyses of metabolomic and transcriptomic data highlighted the activation of lipid metabolism and phenylpropanoid biosynthesis pathways under Cd stress suggesting that these pathways play crucial roles in the detoxification process and in enhancing Cd tolerance in A. manihot. This comprehensive study provides detailed insights into the response mechanisms of A. manihot to Cd toxicity.

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“…In addition, some crucial metabolisms that directly protect cells from Cd stress were highlighted. For example, the metabolism of galactose, lipid, and glutathione in buckwheat (Huo et al, 2023), and the ABC transporter, phenylpropanoid biosynthesis and flavonoid biosynthesis pathway in sorghum (Jiao et al, 2023); the unsaturated fatty acids, amino acids (including phenylalanine), nucleotides, sulfur compounds, flavonoids, glutathione and lignin biosynthetic metabolism in Pistia stratiotes were also implicated (Wei et al, 2023). The metal-transport genes related to Cd uptake, transport, and detoxification have also been extensively investigated.…”

Section: Introductionmentioning

confidence: 99%

Transcriptomics and metabolomics association analysis revealed the responses of Gynostemma pentaphyllum to cadmium

Zhou,

Yao,

Huang

et al. 2023

Front. Plant Sci.

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Gynostemma pentaphyllum an important medicinal herb, can absorb high amounts of cadmium (Cd) which can lead to excessive Cd contamination during the production of medicines and tea. Hence, it is crucial to investigate the response mechanism of G. pentaphyllum under Cd stress to develop varieties with low Cd accumulation and high tolerance. Physiological response analysis, transcriptomics and metabolomics were performed on G. pentaphyllum seedlings exposed to Cd stress. Herein, G. pentaphyllum seedlings could significantly enhance antioxidant enzyme activities (POD, CAT and APX), proline and polysaccharide content subject to Cd stress. Transcriptomics analysis identified the secondary metabolites, carbohydrate metabolism, amino acid metabolism, lipid metabolism, and signal transduction pathways associated with Cd stress, which mainly involved the XTH, EXP and GST genes. Metabolomics analysis identified 126 differentially expressed metabolites, including citric acid, flavonoid and amino acids metabolites, which were accumulated under Cd stress. Multi-omics integrative analysis unraveled that the phenylpropanoid biosynthesis, starch, and sucrose metabolism, alpha-linolenic acid metabolism, and ABC transporter were significantly enriched at the gene and metabolic levels in response to Cd stress in G. pentaphyllum. In conclusion, the genetic regulatory network sheds light on Cd response mechanisms in G. pentaphyllum.

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Temporal physiological, transcriptomic and metabolomic analyses revealed molecular mechanism ofCanna indica’s response to Cr stress

Cited by 4 publications

References 60 publications

Recruitment and metabolomics between Canna indica and rhizosphere bacteria under Cr stress

Recruitment and metabolomics between Canna indica and rhizosphere bacteria under Cr stress

Integrative physiological, transcriptomic, and metabolomic analysis of Abelmoschus manihot in response to Cd toxicity

Transcriptomics and metabolomics association analysis revealed the responses of Gynostemma pentaphyllum to cadmium

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