The process of evaluating the soil quality is complex and is impacted by a variety of physical, chemical, and biological indicators as well as how they interact over time, particularly throughout different cultivation periods. The main objective of the current investigation is to examine how various organic farming practices affect the soil quality indicators at selected organic farms throughout various cultivation times. The study sites in Egypt were selected to represent two great soil groups (Torripsamments and Haplotorrerts). Moreover, the selected farms have different organic cultivation periods and management practices. Soil samples were collected from five different farms and uncultivated soil in each soil great soil group as control. After analyzing soil samples in the lab, physical, chemical, and biological indicators were assessed and used to calculate the Soil Quality Index (SOI). Principal Component Analysis (PCA) was used to obtain the minimum data set needed for soil quality evaluation. The length of organic cultivation in Torripasament raised the SQI value for physical and chemical indicators in the following order: 2 years > 6 years > 10 years > 13 years. Similar to Torripsament, the most sensitive indicators for SQI assessment in Haplotorrert were found to be soil respiration, microbial biomass C, microbial quotient, organic matter, and physical characteristics of soil quality. Results also indicated that organic farming improves most of the studied soil quality indicators of both soils (Torripsamment and Haplotorrerts). Soil respiration, Microbial Biomass (MBC), Microbial Quotient (MQ), organic matter, and physical aspects of soil quality were the most sensitive indicators of soil quality evaluation. Soil quality index (SQI) for Torripsamment was affected mostly by soil biological indicators, while soil physical indicators were the main indicators affecting SQI in Haplotorrerts. The best score for the soil quality index was at 13 years of organic cultivation periods in Torripsamment and at 17 years in Haplotorrerts.