Temporal variations in plasma vitamin K and lipid concentrations and clotting factor activity in humans

Abstract

There is no information available on temporal variability in plasma vitamin K concentrations and its relationship to coagulation processes. We investigated the possible existence of temporal changes in plasma vitamin K and lipid concentrations and activity of clotting factors II, VII, IX, and X and relationships between these variables. Plasma vitamin K and lipid concentrations and clotting factor activity were measured at four-hour intervals for 28 hours in a group of healthy volunteers. Temporal variations existed in plasma vitamin K concentrations, with a mean maximum at 22:00 hr and a mean minimum (32% of the maximum) at 10:00 hr. Plasma triglycerol concentrations mirrored the changes in vitamin K concentrations. Mean factor VII activity was positively correlated with mean total plasma cholesterol concentrations (r = 0.714; P < 0.0001) and with mean plasma low density lipoprotein (LDL) cholesterol concentrations (r = 0.461; P < 0.0001). No distinct correlations were found between plasma vitamin K concentrations and either high density lipoprotein (HDL) or LDL cholesterol concentrations, or between triglycerol, HDL, or LDL cholesterol concentrations and functional activity of factors II, IX, and X. Plasma vitamin K concentrations did not correlate with the functional activity of any of the clotting factors. The presence of a correlation between plasma cholesterol concentrations and factor VII activity for blood samples collected at four-hour intervals suggests that plasma cholesterol concentrations may have a more acute effect on factor VII activity. Temporal variations in plasma vitamin K concentrations indicate that a single time point measurement may be an inappropriate method of establishing vitamin K status in an individual. Am.

Combined deficiency of vitamin K-dependent coagulation factors and extensive hemorrhage

“…Serum vitamin K transport also can significantly alert by serum lipids mostly triglycerides [11]. All of these factors can affect vitamin K-dependent coagulation factors activation and therefore reduce their activity in blood stream [9,11]. In the current patient, high serum lipids, the only factor that we found that can affect patient's coagulation factors and causes severe bleeding and hospitalization.…”

“…Acquired deficiency can results in different conditions including liver disease and malabsorption [8,9]. In the present study, we reported a woman with acquired vitamin K-dependent coagulation factors with extensive menorrhagia.…”

“…In patient's serum sample, chylomicronemia was obvious and when triglyceride was measured, it was very high. Several studies were reported that variations in serum lipids including cholesterol and triglyceride could significantly affect vitamin K-dependent coagulation factors [9]. In the study of Maccallum et al, it was observed that protein C and protein S had significant variations in healthy individuals by increased level of cholesterol and triglyceride [8].…”

“…Acquired VKCFD may be observed in neonatal period as a temporal event [8,9]. This diathesis is more common in patients with chronic liver disease but malnutation, malabsorption and medications are other causes [8][9][10].…”

Relationship between acquired deficiency of vitamin K-dependent clotting factors and hemorrhage

“…Early research indicated that there were temporal variations in lipid concentrations and activities of the four clotting factors which might be influenced by lipid metabolism [31,32] . In the present study, no significant was found in lipid concentrations between the diseased and control subjects, and bivariate correlation analyses showed no significant relationship between each of the four clotting factors and levels of cholesterol and triglycerides (data not shown).…”