Temporary immersion systems (TISs): A comprehensive review

Mirzabe, Amir Hossein; Hajiahmad, Ali; Fadavi, Ali; Rafiee, Shahin

doi:10.1016/j.jbiotec.2022.08.003

Cited by 30 publications

(12 citation statements)

References 207 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The liquid medium allows the plant tissues to have better contact for nutrient absorption enabling better culture growth and development [98]. However, the continuous submersion of plants under the liquid medium in a complete immersion bioreactor system produces unwanted hyperhydricity and asphyxia, undermining normal plant development [99]. Asphyxia is an unwanted physiological condition caused to plants due to low oxygen supply producing malformation in plants by affecting normal growth [100].…”

Section: Limitations Of Ss and Liquid Culturementioning

confidence: 99%

“…Exposure of the plants to gas is essential to avoid unwanted asphyxia in the culture [144]. Plants usually require different oxygen and carbon dioxide concentrations for proper development [99]. Bioreactors fitted with pressure and flow regulators can provide proper gas exposure to the cultures to enhance multiplication and growth.…”

Section: Important Factors Influencing Temporary Immersion System (Tis)mentioning

confidence: 99%

“…Bioreactors fitted with pressure and flow regulators can provide proper gas exposure to the cultures to enhance multiplication and growth. Increasing oxygen and carbon dioxide to a certain level is warranted as they are the crucial components of photosynthesis affecting plant growth and metabolism [99]. The oxygen transfer rate depends on its mass transfer coefficient, which is easily influenced by agitation and air flow rate and the design of the bioreactor [95].…”

Section: Important Factors Influencing Temporary Immersion System (Tis)mentioning

confidence: 99%

“…When exposed to the gas after short immersion, the plants acquire oxygen and carbon dioxide, which are also essential for their development [95]. Optimizing the immersion time and frequency is vital as lengthening the immersion time will increase the hyperhydricity effect, and more prolonged gas exposure will enhance tissue drying and moisture loss, deterring normal development and proliferation [99]. The immersion time and frequency in TIS varied considerably with species and micropropagation process involved.…”

Section: Important Factors Influencing Temporary Immersion System (Tis)mentioning

confidence: 99%

See 3 more Smart Citations

Orchid Micropropagation Using Conventional Semi-Solid and Temporary Immersion Systems: A Review

Nongdam

Beleski

Tikendra

et al. 2023

Plants

View full text Add to dashboard Cite

Orchids, with their astonishingly stunning flowers, dominate the international floricultural market. They are considered prized assets for commercial applications in pharmaceutical and floricultural industries as they possess high therapeutic properties and superior ornamental values. The alarming depletion of orchid resources due to excessive unregulated commercial collection and mass habitat destruction makes orchid conservation measures an extreme priority. Conventional propagation methods cannot produce adequate number of orchids, which would meet the requirement of these ornamental plants for commercial and conservational purposes. In vitro orchid propagation using semi-solid media offers an outstanding prospect of rapidly producing quality plants on a large scale. However, the semi-solid (SS) system has shortcomings with low multiplication rates and high production costs. Orchid micropropagation using a temporary immersion system (TIS) overcomes the limitations of the SS system by reducing production costs and making scaleup and full automation possible for mass plant production. The current review highlights different aspects of in vitro orchid propagation using SS and TIS and their benefits and drawbacks on rapid plant generation.

show abstract

Section: Limitations Of Ss and Liquid Culturementioning

confidence: 99%

Section: Important Factors Influencing Temporary Immersion System (Tis)mentioning

confidence: 99%

Section: Important Factors Influencing Temporary Immersion System (Tis)mentioning

confidence: 99%

Section: Important Factors Influencing Temporary Immersion System (Tis)mentioning

confidence: 99%

See 2 more Smart Citations

Orchid Micropropagation Using Conventional Semi-Solid and Temporary Immersion Systems: A Review

Nongdam

Beleski

Tikendra

et al. 2023

Plants

View full text Add to dashboard Cite

show abstract

“…Studies have indicated that it facilitates faster plant multiplication and is more effective for producing certain secondary metabolites compared to plants grown in soil [ 5 , 13 , 14 , 15 , 16 ]. The key aspect for maximizing the potential of in vitro cultures maintained in the PlantForm TIS is the experimental optimization of process parameters [ 4 , 5 , 15 , 16 , 17 , 18 , 19 , 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Assessment of Lignan Profiling and Biological Activities of Schisandra henryi Leaf and In Vitro PlantForm Bioreactor-Grown Culture Extracts

Jafernik,

Kubica,

Dziurka

et al. 2024

Pharmaceuticals

View full text Add to dashboard Cite

This research’s scope encompassed biotechnological, phytochemical, and biological studies of Schisandra henryi, including investigations into its in vitro microshoot culture grown in PlantForm bioreactors (temporary immersion systems, TISs), as well as extracts from leaves of the parent plant, focusing on anti-inflammatory, antioxidant, anticancer, and antimicrobial activities. The phytochemical analysis included the isolation and quantification of 17 compounds from dibenzocyclooctadiene, aryltetralin lignans, and neolignans using centrifugal partition chromatography (CPC), HPLC-DAD, and UHPLC-MS/MS tandem mass spectrometry with triple quadrupole mass filter methods. Higher contents of compounds were found in microshoots extracts (max. 543.99 mg/100 g DW). The major compound was schisantherin B both in the extracts from microshoots and the leaves (390.16 and 361.24 mg/100 g DW, respectively). The results of the anti-inflammatory activity in terms of the inhibition of COX-1, COX-2, sPLA2, and LOX-15 enzymes indicated that PlantForm microshoot extracts showed strong activity against COX-1 and COX-2 (for 177 mg/mL the inhibition percentage was 76% and 66%, respectively). The antioxidant potential assessed using FRAP, CUPRAC, and DPPH assays showed that extracts from microshoot cultures had 5.6, 3.8, and 3.3 times higher power compared to extracts from the leaves of the parent plant, respectively. The total polyphenol content (TPC) was 4.1 times higher in extracts from the in vitro culture compared to the leaves. The antiproliferative activity against T-cell lymphoblast line Jurkat, breast adenocarcinoma cultures (MCF-7), colon adenocarcinoma (HT-29), and cervical adenocarcinoma (HeLa), showed that both extracts have considerable effects on the tested cell lines. The antimicrobial activity tested against strains of Gram-positive and Gram-negative bacteria and fungi showed the highest activity towards H. pylori (MIC and MBC 0.625 mg/mL).

show abstract

Temporary Immersion Systems in Plant Micropropagation

Ramírez-Mosqueda,

Cruz-Cruz

2024

Methods in Molecular Biology

View full text Add to dashboard Cite

Temporary immersion systems (TISs): A comprehensive review

Cited by 30 publications

References 207 publications

Orchid Micropropagation Using Conventional Semi-Solid and Temporary Immersion Systems: A Review

Orchid Micropropagation Using Conventional Semi-Solid and Temporary Immersion Systems: A Review

Comparative Assessment of Lignan Profiling and Biological Activities of Schisandra henryi Leaf and In Vitro PlantForm Bioreactor-Grown Culture Extracts

Temporary Immersion Systems in Plant Micropropagation

Contact Info

Product

Resources

About