2011
DOI: 10.1007/s12686-011-9410-y
|View full text |Cite
|
Sign up to set email alerts
|

Ten polymorphic microsatellite loci for the Atlantic Silverside, Menidia menidia

Abstract: Ten polymorphic microsatellite loci were isolated from the Atlantic Silverside (Menidia menidia) in order to test hypotheses regarding the role of adaptive phenotypic variation in structuring estuarine populations along coastal North America. Loci were amplified in three multiplex panels requiring a total of four individual PCRs. All loci were highly polymorphic in individuals screened from an estuary in Nova Scotia, and none exhibited significant departures from Hardy-Weinberg equilibrium. The results suggest… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

0
4
0

Year Published

2014
2014
2016
2016

Publication Types

Select...
3

Relationship

0
3

Authors

Journals

citations
Cited by 3 publications
(4 citation statements)
references
References 12 publications
0
4
0
Order By: Relevance
“…Among 58 primers tested, 26 microsatellites screened successfully, 25 resolved as polymorphic and Chum53 was monomorphic in specimens from Villa Victoria. Mean number of alleles in the polymorphic loci was 14.3, SD = 9.38, a mean number 2× higher than the resolved earlier for the species (see [6]; 6.4, SD = 2.5), but similar to those characterized closely related species [17] [18]; and H O and H E 0.47, SD = 0.32 and 0.58, SD = 0.32, respectively. These later values are similar to those previously reported (see [6]; 0.61, SD = 0.19 and 0.63, SD = 0.12, for H O and H E , respectively).…”
Section: Resultsmentioning
confidence: 45%
“…Among 58 primers tested, 26 microsatellites screened successfully, 25 resolved as polymorphic and Chum53 was monomorphic in specimens from Villa Victoria. Mean number of alleles in the polymorphic loci was 14.3, SD = 9.38, a mean number 2× higher than the resolved earlier for the species (see [6]; 6.4, SD = 2.5), but similar to those characterized closely related species [17] [18]; and H O and H E 0.47, SD = 0.32 and 0.58, SD = 0.32, respectively. These later values are similar to those previously reported (see [6]; 0.61, SD = 0.19 and 0.63, SD = 0.12, for H O and H E , respectively).…”
Section: Resultsmentioning
confidence: 45%
“…Larval DNA (DS ranging from 1 to 15 dph) was extracted using a more cost-effective 'salting out' protocol (Sunnucks et al 1996) that yielded useable DNA from larval silversides. Ten polymorphic microsatellite loci for M. menidia (Sbrocco and Barber 2011) were amplified for all parents and offspring in a 10 lL reaction containing 19 PCR buffer, 109 bovine serum albumin, 1.5-3.5 mM MgCl 2 (Table 2), 0.12 mM dNTPs, 0.16 lM of the reverse primer and fluorescently labeled M13 primer (Schuelke 2000), 0.04 lM of the species-specific forward primer and 1 unit Taq polymerase, and 10-40 ng of genomic DNA. Thermal cycling consisted of 5 min at 94°C followed by 35 cycles of 94°C for 30 s, primer specific annealing temperature (T a , Table 2) for 30 s and 72°C for 60 s with a final extension at 72°C for 10 min.…”
Section: Dna Extraction and Amplificationmentioning
confidence: 99%
“…; Murray et al. ), and (iii) the required species‐specific primers for 10 microsatellite loci were already available (Sbrocco and Barber ).…”
Section: Introductionmentioning
confidence: 99%
See 1 more Smart Citation