Ten Years-long Survey on Pathogen Status of Mouse and Rat Breeding Colonies

Detection of Serum Antibodies Using LBC Cell-grown Sialodacryoadenitis (SDA) Virus

“…However, there seemed to be certain problems in antigenicity and reactivity [1]. In Japan, MHV antigen prepared from infected mouse liver has been widly used [4,15,17] and anti-MHV-2 antibody detected in conventionl rat breeder or user colonies was indicative of SDAV infection [4,14]. Using enzymeliked immunosorbent assay with SDAV-TG, Parker's coronavirus (PCV) 8190, MHV-S and MHV-NuU by Machii et al [13] revealed lower reactivities of MHV-S and MHV-NuU antigens for detection of anti-SDAV antibody in rats.…”

“…is widely conducted for detection of infectious diseases in breeding and test colonies of laboratory rats and mice [1,3,4,9,10,13,[15][16][17] . For sialodacryoadenitis virus (SDAV), complement fixation (CF) reaction with mouse hepatitis virus (MHV), which shares common antigens with SDAV, has been routinely used in Japan [4,15,17], while antigenic relatedness between SDAV and MHV has not been thoroughly investigated.…”

“…For sialodacryoadenitis virus (SDAV), complement fixation (CF) reaction with mouse hepatitis virus (MHV), which shares common antigens with SDAV, has been routinely used in Japan [4,15,17], while antigenic relatedness between SDAV and MHV has not been thoroughly investigated. Of SDAV antigen used in the U. S. A. certain problems in its preparation procedures and specificity have been described [1,2,161.…”

Antigenic Characterization of <I>Pasteurella pneumotropica</I>Isolated from Mice and Rats

“…Past eurella pneumotropica has been isolated from various animal species including mice, rats, Syrian hamsters, guinea pigs and dogs [1,8,10,13], and regarded as a causative agent of pneumonia [1][2][3]7,8], subcutaneous abscess [10,19,20,22], mastitis [6,19], conjunctivitis [1,11,20], metritis [1], abortion [21] and urocystitis [1]. It is well known that mice and rats are often healthy carriers of the organism [4,5,7,8,13,17,18], however, host-specific parasitism of the organism has not yet been investigated except our previous study in which rat strains were shown to be inf ective to rats but rarely to mice, while mouse strains were highly inf ective to both animals [12].…”

“…It is well known that mice and rats are often healthy carriers of the organism [4,5,7,8,13,17,18], however, host-specific parasitism of the organism has not yet been investigated except our previous study in which rat strains were shown to be inf ective to rats but rarely to mice, while mouse strains were highly inf ective to both animals [12].…”