Tepidibacter thalassicus gen. nov., sp. nov., a novel moderately thermophilic, anaerobic, fermentative bacterium from a deep-sea hydrothermal vent

Abstract

Tepidibacter thalassicus gen. nov., sp. nov., a novel moderately thermophilic, anaerobic, fermentative bacterium from a deep-sea hydrothermal vent Micro-organisms inhabiting deep-sea hydrothermal vents are physiologically and phylogenetically diverse, and are represented by members of the Bacteria and the Archaea (Jeanthon, 2000). Chemo-organotrophic thermophilic anaerobic bacteria isolated from these extreme environments include members of the order Thermotogales -Thermosipho melanesiensis ( et al., 2002b). In this article, we report the isolation and characterization of an anaerobic, moderately thermophilic, fermentative, endospore-forming micro-organism (strain SC 562 T ) belonging to a novel genus within the Bacillus-Clostridium subphylum of the Bacteria. Strain SC 562T was isolated from a sample of the outer part of a chimney-like structure (a black smoker) covered with tubes and specimens of the polychaetous annelid Alvinella spp. The sample was collected during the AMISTAD cruise at the 13u N hydrothermal field on the East-Pacific Rise at a depth of 2650 m, as described previously (Slobodkin et al., 2001). An enrichment culture was initiated by inoculating 10 % (w/v) of the sample into anaerobically prepared, bicarbonate-buffered, sterile (135 uC, 1 h)

Abundance and diversity of octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX)-metabolizing bacteria in UXO-contaminated marine sediments

“…They fermented peptone and glucose into propionate and butyrate. Strain UXO3‐5, a typical strain of group III bacteria, was closely related to the two recognized species Tepidibacter thalassicus DSM 15285 T (Slobodkin et al , 2003) and Tepidibacter formicigens DSM 15518 T (Urios et al , 2004), with 96.8−97.3% similarity in their 16S rRNA gene (Fig. 1).…”

Bacterial and archeal community composition in hot springs from Indo-Burma region, North-east India

“…The presence of sequence reads from bacterial taxa Tepidibacter sp., Ignavibacterium sp., Teribacillus sp., Dechloromonas sp., could be the representatives of novel species within these genera. The genus Tepidibacter ( Firmicutes ) was proposed (Slobodkin et al 2003) with three reported type species i.e. Tepidibacter thalassicus, Tepidibacter formicigenes and Tepidibacter mesophilus (Tan et al 2012).…”

Presence of glucose, xylose, and glycerol fermenting bacteria in the deep biosphere of the former Homestake gold mine, South Dakota

“…Considering these limitations, we have isolated glucose-, xylose-, or glycerol-fermenting bacteria from the extreme deep biosphere environment of the former Homestake gold mine, Lead, SD. There are very few reports on subsurface isolates utilizing glucose for ethanol production (Alain et al, 2002; Slobodkin et al, 2003; Slobodkina et al, 2008) but the information on xylose and glycerol fermenting deep subsurface microbes is relatively scarce. Deep subsurface microbes, Tepidibacter thalassicus and Clostridium tepidiprofundi sp.…”

“…Deep subsurface microbes, Tepidibacter thalassicus and Clostridium tepidiprofundi sp. nov., fermented glucose into ethanol, acetate, and H 2 (Slobodkin et al, 2003; Slobodkina et al, 2008). Another strain, Caminicella sporogenes gen. nov., sp.…”