Terahertz spectroscopy for quantification of free water and bound water in leaf

Zang, Ziyi; Li, Zaoxia; Lu, Xing-Xing; Liang, Jie; Wang, Jie; Cui, Hong‐Liang; Yan, Shihan

doi:10.1016/j.compag.2021.106515

Cited by 20 publications

(9 citation statements)

References 51 publications

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“…This is consistent with the presence of bound water. Due to the reduced freedom of movement, the spectrum of bound water behaves as if it were a lower temperature than bulk water [ 3 , 18 ].…”

Section: Discussionmentioning

confidence: 99%

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Tissue Characterization Using Synchrotron Radiation at 0.7 THz to 10.0 THz with Extended ATR Apparatus Techniques

Vilagosh

Appadoo

Foroughimehr

et al. 2022

Sensors

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The attenuated total reflection (ATR) apparatus, with an added partial reflection/partial transmission mode, was used to demonstrate a novel way of characterizing water-based substances at 0.7 to 10.0 THz at the Australian Synchrotron THz-far infrared beamline. The technique utilized a diamond-crystal-equipped ATR to track temperature-dependent changes in reflectance. A “crossover flare” feature in the spectral scan was noted, which appeared to be a characteristic of water and water-dominated compounds. A “quiet zone” feature was also seen, where no temperature-dependent variation in reflectance exists. The variation in these spectral features can be used as a signature for the presence of bound and bulk water. The method can also potentially identify the presence of fats and oils in a biological specimen. The technique requires minimal sample preparation and is non-destructive. The presented method has the promise to provide a novel, real-time, low-preparation, analytical method for investigating biological material, which offers avenues for rapid medical diagnosis and industrial analysis.

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Section: Discussionmentioning

confidence: 99%

“…The THz spectra of biological tissues and specimens, such as microscopic organisms, bacteria, and cultured cells, can yield information regarding tissue hydration, bound water content, the presence of melanin, and fat content [ 1 , 2 , 3 ]. Attenuated total reflection (ATR) is a well-established spectral technique.…”

Section: Introductionmentioning

confidence: 99%

Tissue Characterization Using Synchrotron Radiation at 0.7 THz to 10.0 THz with Extended ATR Apparatus Techniques

Vilagosh

Appadoo

Foroughimehr

et al. 2022

Sensors

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“…Stable water content is essential to maintain the cornea in a normal state; a 10% change in water content results in pathological conditions [ 13 ]. Two natural states of water in biological tissues are known as free “bulk” water (i.e., freely moving water molecules) and bound water [ 31 ] which is associated with tissue molecular components and is not movable. As the name implies, free “bulk” water can move freely within the cell whereas bound water is immobilised by hydrogen bonds in more subtle molecular contact with cellular constituents.…”

Section: Introductionmentioning

confidence: 99%

“…The bound water is converted into free water when strong stimulation is applied to the tissue [ 32 ]. At THz frequencies, the result is that bound water has a lower absorption coefficient and a higher refractive index when compared to free water at the same temperature [ 31 ]. Water illustrates high absorption and reflection coefficient values in the 0.3 to 3 THz frequency range [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Investigating the Impact of Synchrotron THz Radiation on the Corneal Hydration Using Synchrotron THz-Far Infrared Beamline

Foroughimehr

Vilagosh

Yavari

et al. 2022

Sensors

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Due to increasing interest in imaging, industrial, and the development of wireless communication operating at THz frequencies, it is crucial to ascertain possible health impacts arising from exposure to THz radiation. This paper reports on the pilot study of transmittance and absorbance spectra of the porcine cornea following THz frequency irradiation at a synchrotron THz/Far-IR beamline. The exposure period was 4 hours. One cornea was exposed to the radiation, with a second cornea acting as a control.An Attenuated Total Reflection (ATR) apparatus was used, and the frequency range of 2.4 to 8 THz was selected to evaluate any changes. It was found that the synchrotron THz radiation intensities are too low to produce induced corneal injury, but may lead to subtle changes in the state of water. Our results suggest that THz spectroscopy is a promising modality for corneal tissue hydration sensing.

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“…Due to the strong absorption of THz wave by water [ 22 , 23 , 24 , 25 ], and the slow development of high power THz wave source [ 26 , 27 , 28 , 29 ], THz microfluidic detection technology [ 30 , 31 , 32 ] and THz attenuated total reflection detection technology [ 33 , 34 , 35 , 36 , 37 ], the advancement of THz spectroscopy detection technology for water-containing biological substances is slow. In this paper, we investigated the absorption characteristics of L-arginine aqueous solution in THz band through simulation and experimental detection, because it is a semi-essential amino acid in the body, and its main physiological functions are lowering blood pressure [ 38 , 39 , 40 ], regulating blood glucose [ 41 ], protecting the liver [ 42 ], enhancing immunity and limiting tumor growth [ 43 , 44 , 45 , 46 , 47 ], benefit embryo survival and foetal development [ 48 , 49 , 50 , 51 ], accelerating wound healing [ 52 , 53 ].…”

Section: Introductionmentioning

confidence: 99%

Experimental Detection and Simulation of Terahertz Spectra of Aqueous L-Arginine

Hou

Wang

et al. 2022

Biosensors

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Terahertz (THz) wave is a good candidate for biological sample detection, because vibration and rotation energy levels of biomolecule are in THz band. However, the strong absorption of THz wave by water in biological samples hinders its development. In this paper, a method for direct detection of THz absorption spectra of L-arginine suspension was proposed by using a strong field THz radiation source combined with a polyethylene cell with micrometer thickness in a THz time-domain spectroscopy system. And the THz absorption spectrum of L-arginine solution was simulated by the density functional theory and the simulation result is in good agreement with the experimental results. Finally, the types of chemical bond interaction that cause the absorption peak are identified based on the experimental and simulation results. This work paves a way to investigate the THz absorption spectra and intramolecular interactions of aqueous biological samples.

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Terahertz spectroscopy for quantification of free water and bound water in leaf

Cited by 20 publications

References 51 publications

Tissue Characterization Using Synchrotron Radiation at 0.7 THz to 10.0 THz with Extended ATR Apparatus Techniques

Tissue Characterization Using Synchrotron Radiation at 0.7 THz to 10.0 THz with Extended ATR Apparatus Techniques

Investigating the Impact of Synchrotron THz Radiation on the Corneal Hydration Using Synchrotron THz-Far Infrared Beamline

Experimental Detection and Simulation of Terahertz Spectra of Aqueous L-Arginine

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