Teriparatide improves alveolar bone modelling after tooth extraction in orchiectomized rats

Effect of Topical PTH 1-34 Functionalized to Biogran® in the Process of Alveolar Repair in Rats Submitted to Orchiectomy

“…In laser confocal microscopy, for the mineral apposition rate (MAR), in which we observed the calcium precipitation on bone surface, we observed an intragroup relationship for SHAM BG-PTH and ORQ BG-PTH in which the values presented increased, demonstrating a clear improvement in bone repair after biomaterial insertion with local PTH. This can be compared with the systemic effect of teriparatide described in the study by De Oliveira Puttini et al 2019 [ 29 ], in which the osteopenic group treated with systemic PTH also showed excellent results. For AON, the group that obtained the highest result was SHAM CLOT, with greater balance between new (red) and old (green) bone formation; the SHAM BG-PTH and ORQ BG-PTH groups were similar to it.…”

“…In addition, TRAP showed mild labeling for the treated groups, showing a local anabolic effect of teriparatide in opposition to osteoclast resorption activity. Studies of de Oliveira et al and Oliveira Puttini et al showed that the use of systemic teriparatide after exodontia in orchiectomized rats led to increased expression for Wnt and osteocalcin proteins [ 59 ], a balance between bone formation and resorption for OPG and RANKL proteins [ 29 ] and lower labeling for TRAP protein [ 59 ], indicating increased repair tissue formation and less alveolar bone resorption. Therefore, from the results presented, it can be inferred that the use of Biogran ® promoted slight improvement in rat alveolar repair and that, with the addition of PTH 1-34 topic, this condition tends to improve, resembling bone repair by clot formation.…”

“…The improvement of biomaterials properties, through the incorporation of biomolecules that may evoke cellular responses that will be contributing to the repair process, is a challenge that has presented interesting results. Teriparatide that already presented important results through systemic administration has also presented an activation of the osteoblasts receptor and has become an attractive biomolecule that may be delivered in the repair site and may develop interesting responses activating bone formation [ 12 , 13 , 14 , 28 , 29 ]. Studies with topical application of teriparatide presented positive responses in bone formation [ 17 ].…”

β-catenin and Its Relation to Alveolar Bone Mechanical Deformation – A Study Conducted in Rats With Tooth Extraction

“…The rat model has been widely used for studies in dentistry, including those which involve periodontal diseases, alveolar bone healing, tooth movement, osteogenesis, osseointegration, use of biomaterials, and altered occlusal loadings, for example (Okamoto and de Russo, 1973;Shimomoto et al, 2007;Walker et al, 2008;Wan et al, 2012;Xu et al, 2016;Zeng et al, 2016;Koutouzis et al, 2017;Lee et al, 2017;Hassumi et al, 2018;Palin et al, 2018;Uslu et al, 2018;Wang et al, 2018). Besides that, tooth extraction, inclosing incisor extraction, has been considered as a classical model of preclinical studies and has been applied in many studies with different aims in the last years (Avivi-Arber et al, 2010;Shoji et al, 2010;Boonyagul et al, 2014;Curra et al, 2016;Hassumi et al, 2018;de Oliveira Puttini et al, 2019). Medical and dental science has benefited from the rat as a model through the investigation of pathological conditions affecting craniofacial morphology.…”

Bone remodeling during alveolar repair is impaired by RANKL antagonist

“…Thus, at 14 days after extraction of the right upper incisor it was administered by the intramuscular route 20 mg/kg of fluorocromo calcein. After 28 days (42 days after extraction), the alizarin red fluorochrome was administered intramuscularly in the amount of 20 mg/kg (Oliveira et al, 2019).…”