Term development of caprine embryos derived from immature oocytes in vitro

Keskintepe, Levent; Darwish, Gamal M.; Kenimer, A.T.; Brackett, Benjamin G.

doi:10.1016/0093-691x(94)90690-k

Cited by 47 publications

(21 citation statements)

References 23 publications

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“…no. 230-614OPJ, GIBCO Laboratories, Grand Island, NY 14072) 100 pg LH (NIADDK-oLH-26) ml-', 0.5 pg FSH ml-', and 1 pg Estradiol-17-P (Sigma) ml-', 5.5 mg Glucose ml-', 3.6 mg NaHCO, ml-', 112 pg Pyruvateml-' and 50 pg Gentamycinml-' (Keskintepe et al, 1994a).…”

Section: Oocyte Retrieval and Maturationmentioning

confidence: 99%

“…Culture of presumptive zygotes and embryos was carried out by the methods reported in our earlier experiments (Keskintepe et al, 1994a;Keskintepe et al, 1994b). The medium was TCM 199 with 25 mM HEPES (HM-199, Sigma) supplemented with 10% fetal bovine serum (FBS).…”

Section: In Vitro Culturementioning

confidence: 99%

“…Embryo transfer was performed as previously described (Keskintepe et al, 1994a) with the exception that embryos in this work were deposited directly into the uterine horns instead of oviducts. After entry of the peritoneal cavity, access into the uterine lumen was afforded by puncture through the myometrium using an atraumatic surgical needle.…”

Section: Synchronizationmentioning

confidence: 99%

“…Difficulties in obtaining adequate numbers of oocytes have been overcome by recent progress in oocyte maturation following acquisition of ovaries at slaughter (Martin0 et al, 1994; 1 1 -X 1994b). It is now possible to obtain live kids following transfer of embryos completely produced by IVMFC of goat oocytes (Keskintepe et al, 1994a). …”

Section: Introductionmentioning

confidence: 99%

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Procedural improvements for in vitro production of viable uterine stage caprine embryos

Keskintepe

Luvoni

Rzucidlo

et al. 1996

Small Ruminant Research

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Efforts to improve proportions of caprine immature oocytes developing into viable uterine-stage embryos in vitro involved study of 1924 oocytes in experiments designed to examine influences of fertilization media, sperm incubation temperatures, sperm treatment procedures, different protein supplementations, and different insemination intervals. Oocytecumulus complexes (OCCs) were matured during 27 h in TCM-199 supplemented with 20% FBS, 100 pug LH ml-', 0.5 pg FSH ml-', and 1 pg Estradiol-17-P ml-' at 38.5"C in a humidified 5% CO,, 5% O,, and 90% N, atmosphere. Freshly collected sperm were washed and incubated at either 22°C or 38.5"C for 5 h and then treated with either 0.1 PM calcium ionophore A23187 for 1 min, or with 7.35 mM calcium lactate in the presence of oocytes during the insemination interval, or with 100 pg heparin +2 mM caffeineml -' for 15 min. 'The interval for insemination was experimentally varied, i.e. 14 or 24 h. Results showed that: (a) when used as a fertilization medium mDM supported more blastocyst development than TALP (10.5% vs. O%, P < 0.05); (b) incubation temperatures of 22°C or 38.5"C prepared goat spermatozoa equally for capacitation in mDM containing 20% FBS; (c> when oocytes were inseminated with sperm incubated in mDM with 20% FBS and capacitated with calcium lactate more embryos reached the blastocyst stage (P < 0.05) than after incubation in the same conditions but after sperm capacitation with heparin, and A23187 (31.8% vs. 24.2% and 10.2%, respectively; (d) a 24 h insemination interval was not superior to 14 h when sperm were incubated with either 20% FBS or 6 mg BSAml-' and capacitated with calcium lactate (P > 0.05). Three morulae resulting from the best conditions in this work (FBS, calcium lactate, 14 h insemination) were transferred into the uterine horn ipsilateral to the corpus luteum of a recipient and two normal female kids were born after normal gestation. This is the first report in which it has been possible to consistently take caprine development to the blastocyst stage in vitro, and to obtain offspring following uterine transfer. Methodology reported here should facilitate implementation of new reproductive and genetic strategies in goat breeding.

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Section: Oocyte Retrieval and Maturationmentioning

confidence: 99%

Section: In Vitro Culturementioning

confidence: 99%

Section: Synchronizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Procedural improvements for in vitro production of viable uterine stage caprine embryos

Keskintepe

Luvoni

Rzucidlo

et al. 1996

Small Ruminant Research

Self Cite

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“…Sera are usually added to the IVM medium to the rate of 10-20% and are known to contain hormones, trace elements, growth factors [7], chelate heavy metal cations and serves as an osmolyte, as well as surfactant [8]. In all the experiments, maturation medium contained, bovine follicular fluid (BFF) [9], estrous goat serum (EGS) [10,11], estrous sheep serum (ESS) [11,12], estrous cow serum (ECS) [2], fetal bovine serum (FBS) [11,13] and bovine serum albumin (BSA) [14] as protein supplements. The maturation medium and Capsule Media Supplemented with human menopausal serum, estrous sheep serum and estrous goat serum supported better rates of in vitro maturation, in vitro fertilization and embryo development.…”

Section: Introductionmentioning

confidence: 99%

In vitro maturation of ovine oocytes using different maturation media: effect of human menopausal serum

Shabankareh

Sarsaifi

Mehrannia

2010

J Assist Reprod Genet

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Objective To compare the effect of human menopausal serum with estrous sheep serum, estrous goat serum, ovine follicular fluid and bovine follicular fluid on in vitro maturation, in vitro fertilization and embryo development of sheep oocytes Method (s) Oocytes were treated in culture with different sera and follicular fluids supplemented media to examine effects on embryo development. Results Basic culture medium supplemented with human menopausal serum, estrous sheep serum and estrous goat serum supported better rates of in vitro maturation, in vitro fertilization and embryo development. Ovine follicular fluid and bovine follicular fluid supplementations supported similar rates of In vitro maturation, In vitro fertilization and embryo development which were lower than those supported by human menopausal serum, estrous sheep serum, estrous goat serum and control medium.Conclusion Human menopausal serum, estrous sheep serum, and estrous goat serum resulted in higher maturation, fertilization and embryo development than ovine follicular fluid, bovine follicular fluid and control media.

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Embryo Transfer andin vitroFertilization

Hasler¹

2007

Comparative Reproductive Biology

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Term development of caprine embryos derived from immature oocytes in vitro

Cited by 47 publications

References 23 publications

Procedural improvements for in vitro production of viable uterine stage caprine embryos

Procedural improvements for in vitro production of viable uterine stage caprine embryos

In vitro maturation of ovine oocytes using different maturation media: effect of human menopausal serum

Embryo Transfer andin vitroFertilization

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