Terminal Regions of Wheat Chromosomes Select Their Pairing Partners in Meiosis

Corredor, Eduardo; Lukaszewski, Adam J.; Pachón, Paula; Allen, Diana C.; Naranjo, T.

doi:10.1534/genetics.107.078121

Cited by 76 publications

(70 citation statements)

References 55 publications

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“…This hypothesis is supported by the fact that chromosomes with terminal deletions show increased CO number in regions that were formerly proximal Qi et al 2002). Higher CO rates in distal subtelomeric regions of chromosomes have been related to the onset of meiosis with the bouquet formation occurring in the vicinity of these regions thereby favoring CO formation (Corredor et al 2007). This feature, however, is a common feature of meiosis and thus also appears in species with small genomes that do not show such contrast in CO rate between distal and proximal regions.…”

Section: Discussionmentioning

confidence: 99%

Detailed Recombination Studies Along Chromosome 3B Provide New Insights on Crossover Distribution in Wheat (Triticum aestivum L.)

et al. 2009

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In wheat (Triticum aestivum L.), the crossover (CO) frequency increases gradually from the centromeres to the telomeres. However, little is known about the factors affecting both the distribution and the intensity of recombination along this gradient. To investigate this, we studied in detail the pattern of CO along chromosome 3B of bread wheat. A dense reference genetic map comprising 102 markers homogeneously distributed along the chromosome was compared to a physical deletion map. Most of the COs (90%) occurred in the distal subtelomeric regions that represent 40% of the chromosome. About 27% of the proximal regions surrounding the centromere showed a very weak CO frequency with only three COs found in the 752 gametes studied. Moreover, we observed a clear decrease of CO frequency on the distal region of the short arm. Finally, the intensity of interference was assessed for the first time in wheat using a Gamma model. The results showed m values of 1.2 for male recombination and 3.5 for female recombination, suggesting positive interference along wheat chromosome 3B.

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Section: Discussionmentioning

confidence: 99%

Detailed Recombination Studies Along Chromosome 3B Provide New Insights on Crossover Distribution in Wheat (Triticum aestivum L.)

et al. 2009

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“…3). In wheat, the frequency of recombination is suppressed in the vicinity of centromeres (Corredor et al, 2007). Therefore, it is highly probable that the 3H genetic map was distorted due to Table 2.…”

Section: Comparison Of the 3h Physical And Genetic Mapsmentioning

confidence: 99%

Dissection of barley chromosome 3H in common wheat and a comparison of 3H physical and genetic maps

et al. 2009

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We used the gametocidal system to dissect a barley chromosome 3H added to common wheat. The gametocidal system induced chromosomal structural changes in the 3H addition line of common wheat, and we cytologically screened for rearranged chromosomes involving the 3H chromosome by in situ hybridization (FISH/GISH). We established 50 common wheat lines carrying single rearranged (or dissected) 3H chromosomes of independent origin. The dissected 3H chromosomes were either deletions or translocations with wheat chromosomes, and their breakpoints were in the centromere/the long arm/the short arm in a rough ratio of 1:2:2. We used these so-called 3H dissection lines to map 36 EST markers that were polymorphic between euploid common wheat and the 3H addition line and that had been used for the construction of a 3H genetic map. We conducted PCR analysis to detect the EST markers in the dissection lines. The results of the PCR analysis, which mostly corresponded to the retained or lost segments of the dissected 3H chromosomes, allowed us to place the 36 EST markers into 20 chromosomal regions flanked by the breakpoints of the dissected chromosomes. We compared this physical map constructed in this study with a 3H genetic map constructed using the same EST markers. The order of all EST markers was consistent between the two maps. We briefly discuss on the advantage of the physical mapping using dissection lines over genetic mapping.

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“…The main regulator, Ph1, was discovered 50 years ago (Riley and Chapman, 1958;Sears and Okamoto, 1958) and only recently characterized at the molecular level (Griffiths et al, 2006;Al-Kaff et al, 2007). However, the very peculiar nature of the Ph1 locus, which consists of a cluster of cyclin-dependant kinases (cdk-like genes) and a segment of subtelomeric heterochromatin, does not readily explain the multiple cytological effects attributed to Ph1 (Feldman, 1993;Mikhailova et al, 1998;Martinez-Perez et al, 2003;Prieto et al, 2005;Corredor et al, 2007). On the other hand, Ph2, another suppressor of homoeologous associations (Upadhya and Swaminathan, 1967;MelloSampayo, 1971), was shown to affect synaptic progression (Martinez et al, 2001) but the genes responsible for the phenotype are still to be identified (Sutton et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Genetic Regulation of Meiotic Cross-Overs between Related Genomes inBrassica napusHaploids and Hybrids

et al. 2009

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Although the genetic regulation of recombination in allopolyploid species plays a pivotal role in evolution and plant breeding, it has received little recent attention, except in wheat (Triticum aestivum). PrBn is the main locus that determines the number of nonhomologous associations during meiosis of microspore cultured Brassica napus haploids (AC; 19 chromosomes). In this study, we examined the role played by PrBn in recombination. We generated two haploid 3 euploid populations using two B. napus haploids with differing PrBn (and interacting genes) activity. We analyzed molecular marker transmission in these two populations to compare genetic changes, which have arisen during meiosis. We found that cross-over number in these two genotypes was significantly different but that cross-overs between nonhomologous chromosomes showed roughly the same distribution pattern. We then examined genetic recombination along a pair of A chromosomes during meiosis of B. rapa 3 B. napus AAC and AACC hybrids that were produced with the same two B. napus genotypes. We observed significant genotypic variation in cross-over rates between the two AAC hybrids but no difference between the two AACC hybrids. Overall, our results show that PrBn changes the rate of recombination between nonhomologous chromosomes during meiosis of B. napus haploids and also affects homologous recombination with an effect that depends on plant karyotype.

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Terminal Regions of Wheat Chromosomes Select Their Pairing Partners in Meiosis

Cited by 76 publications

References 55 publications

Detailed Recombination Studies Along Chromosome 3B Provide New Insights on Crossover Distribution in Wheat (Triticum aestivum L.)

Detailed Recombination Studies Along Chromosome 3B Provide New Insights on Crossover Distribution in Wheat (Triticum aestivum L.)

Dissection of barley chromosome 3H in common wheat and a comparison of 3H physical and genetic maps

Genetic Regulation of Meiotic Cross-Overs between Related Genomes inBrassica napusHaploids and Hybrids

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