Terminalia bellirica fruit extracts: in-vitro antibacterial activity against selected multidrug-resistant bacteria, radical scavenging activity and cytotoxicity study on BHK-21 cells

Dharmaratne, M. Priyanga Jayamal; Manoraj, Amirthasingam; Thevanesam, Vasanthi; Ekanayake, Asela; Kumar, N. Savitri; Liyanapathirana, Veranja; Abeyratne, Eranga; Bandara, B. M. Ratnayake

doi:10.1186/s12906-018-2382-7

Cited by 51 publications

(38 citation statements)

References 24 publications

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“…In contrast, in spontaneously hypertensive rats, the H 2 S-induced actions scarcely depend on nitric oxide release; also, the nitric oxide effects are largely H 2 S-independent and represent the first step for understanding pathophysiological mechanisms of nitric oxide/H 2 S interplays under both normotensive and hypertensive conditions [31]. Terminalia bellirica is also shown to have antihypertensive [17] and antioxidant [32] activity. Curcumae aeruginosae known by the local name of black temu and Amomi fructus (cardamom) have been proven to have antioxidant activity [18][19][20].…”

Section: Discussionmentioning

confidence: 99%

Acute and Subchronic Oral Toxicity Study of Polyherbal Formulation Containing Allium sativum L., Terminalia bellirica (Gaertn.) Roxb., Curcuma aeruginosa Roxb., and Amomum compactum Sol. ex. Maton in Rats

Sholikhah

Mustofa

Nugrahaningsih

et al. 2020

BioMed Research International

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The polyherbal formulation containing Allium sativum L., Terminalia bellirica (Gaertn.) Roxb., Curcuma aeruginosa Roxb., and Amomum compactum Sol ex. Maton has been used for hypertension treatment empirically. Our previous study showed its blood pressure-lowering effect on a rat model of hypertension. However, toxicity data were not available for this polyherbal formulation. This study is aimed at evaluating the acute and subchronic oral toxicity of the polyherbal formulation in rats. The acute toxicity study was conducted on 6 female Wistar rats using the fixed-dose method for the treatment group and 5 female Wistar rats for the control. The single dose of 2,000 mg/kg of the polyherbal formulation was given orally. There were no significant toxic effects and no death observed until the end of the study, and it was showed that the lethal dose 50% (LD50) of the polyherbal formulation was estimated to be more than 2,000 mg/kg. The macroscopic and microscopic examination of vital organs showed no symptoms of toxicity. At the subchronic toxicity study, the polyherbal formulation with 3 dose variations of 252 mg/kg, 1,008 mg/kg, and 4,032 mg/kg was administered for 91 days orally. The lowest dose of 252 mg/kg is equivalent to the daily recommended dose for a human. There were no significant toxic effects observed at all doses on physical sign and symptoms, weight gain, food intake, hematological parameters, biochemical parameters, and macroscopic and microscopic examination of organs. These findings showed that the short- and long-term oral administration of the polyherbal formulation is safe to use within its dose recommendation.

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Section: Discussionmentioning

confidence: 99%

Acute and Subchronic Oral Toxicity Study of Polyherbal Formulation Containing Allium sativum L., Terminalia bellirica (Gaertn.) Roxb., Curcuma aeruginosa Roxb., and Amomum compactum Sol. ex. Maton in Rats

Sholikhah

Mustofa

Nugrahaningsih

et al. 2020

BioMed Research International

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“…Roxb. ( Dharmaratne et al., 2018 ) and Terminalia chebula Retz. ( Sharma et al., 2012 ) fruit extracts.…”

Section: Updates On the Research On Terminalia Spmentioning

confidence: 99%

“…Roxb. fruits possess antibacterial activity without having cytotoxicity ( Dharmaratne et al., 2018 ). A recent study showed that the aqueous acetone extract of T. bellirica (Gaertn.)…”

Section: Terminalia Sp Medicinal Potential (Folk Medicinal mentioning

confidence: 99%

“…Roxb. dried fruits were used to obtain direct and sequential dichloromethane, methanol, and water extracts, and to evaluate their antibacterial activity against 16 strains of methicillin-resistant Staphylococcus aureus , spectrum β-lactamase producing Escherichia coli , and methicillin-resistant Acinetobacter sp., Klebsiella pneumoniae , and Pseudomonas aureginosa ( Dharmaratne et al., 2018 ). The minimum inhibitory concentration values showed that all aqueous and methanol extracts have antibacterial activity with values ranging from 0.25 to 4 mg/mL against all strains tested, which indicates that further studies are needed to test the antimicrobial potential of Terminalia sp.…”

Section: Pharmacological Effects Of Active Constituents From mentioning

confidence: 99%

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Plants of the Genus Terminalia: An Insight on Its Biological Potentials, Pre-Clinical and Clinical Studies

et al. 2020

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The evaluation and confirmation of healing properties of several plant species of genus Terminalia based on their traditional uses and the clinical claims are of utmost importance. Genus Terminalia has received more attention to assess and validate the therapeutic potential and clinical approval due to its immense folk medicinal and traditional applications. Various species of Terminalia genus are used in the form of herbal medicine and formulations, in treatment of diseases, including headache, fever, pneumonia, flu, geriatric, cancer, to improve memory, abdominal and back pain, cough and cold, conjunctivitis, diarrhea, heart disorder, leprosy, sexually transmitted diseases, and urinary tract disorders. These are reported to possess numerous biological properties, counting: antibacterial, antifungal, antiinflammatory, antiviral, antiretroviral, antioxidant, and antipa7rasitic. This current research review aims to update the detailed biological activities, pre-clinical and clinical studies of various extracts and secondary metabolites from several plant species under the genus Terminalia , along with information on the traditional uses and chemical composition to develop a promising strategy for their potential applications in the form of medicine or use in modern drug formulations for treating diseases like pneumonia, flu, and other types of viral infections or controlling human contagions.

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“…Wight & Arn. have been particularly well studied [21]. In contrast, the Australian Terminalia species remain relatively poorly examined.…”

Section: Terminalia Ferdinandiana Fruit and Leaf Extracts Inhibit Metmentioning

confidence: 99%

Terminalia ferdinandiana Fruit and Leaf Extracts Inhibit Methicillin-Resistant Staphylococcus aureus Growth

et al. 2019

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The development of multiple antibiotic–resistant bacteria has vastly depleted our repertoire of effective antibiotic chemotherapies. The development of multi-β-lactam-resistant strains are particularly concerning due to our previous reliance on this class of antibiotics because of their initial efficacy and broad-spectrum activity. With increases in extended-spectrum β-lactam-resistance and an expanded resistance to other classes of antibiotics, there is an urgent need for the development of effective new antibiotic therapies. Terminalia ferdinandiana is an endemic Australian plant known for its high antioxidant and tannin contents. T. ferdinandiana fruit and leaf extracts have strong antibacterial activity against a wide variety of bacterial pathogens. However, T. ferdinandiana extracts have not been tested against ESBL and MRSA antibiotic-resistant pathogens. An objective of this study was to screen T. ferdinandiana fruit and leaf extracts for bacterial growth inhibitory activity by disc diffusion assay against β-lactam-sensitive and -resistant E. coli strains and against methicillin-sensitive and -resistant S. aureus. The minimum inhibitory concentration (MIC) was quantified by liquid dilution techniques. The fruit methanolic extract, as well as the methanolic, aqueous, and ethyl acetate leaf extracts strongly inhibited the growth of the MRSA, with MICs as low as 223 µg/mL. In contrast, the extracts were ineffective inhibitors of ESBL growth. Metabolomic fingerprint analysis identified a diversity and relative abundance of tannins, flavonoids, and terpenoids, several of which have been reported to inhibit MRSA growth in isolation. All extracts were nontoxic in the Artemia nauplii and HDF toxicity assays, further indicating their potential for medicinal use.

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Terminalia bellirica fruit extracts: in-vitro antibacterial activity against selected multidrug-resistant bacteria, radical scavenging activity and cytotoxicity study on BHK-21 cells

Cited by 51 publications

References 24 publications

Acute and Subchronic Oral Toxicity Study of Polyherbal Formulation Containing Allium sativum L., Terminalia bellirica (Gaertn.) Roxb., Curcuma aeruginosa Roxb., and Amomum compactum Sol. ex. Maton in Rats

Acute and Subchronic Oral Toxicity Study of Polyherbal Formulation Containing Allium sativum L., Terminalia bellirica (Gaertn.) Roxb., Curcuma aeruginosa Roxb., and Amomum compactum Sol. ex. Maton in Rats

Plants of the Genus Terminalia: An Insight on Its Biological Potentials, Pre-Clinical and Clinical Studies

Terminalia ferdinandiana Fruit and Leaf Extracts Inhibit Methicillin-Resistant Staphylococcus aureus Growth

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