Terminalia catappa leaf extracts inhibited metastasis of A2058 and A375 melanoma cells via downregulating p-Src and β-catenin pathway in vitro

Abstract

Background: Melanoma is a highly aggressive, lethal, and malignant cancer. Once diagnosed early, it can be easily removed and cured with satisfaction. Although many methods such as surgery, chemotherapy, radiotherapy, and immunotherapy have been used to treat this disease at an advanced stage, the outcomes are poor. Terminalia catappa leaves have been shown to have various biological benefits, including antitumor activity. The specific effects and molecular mechanisms of Terminalia catappa leaf in treating A2058 and A375 melanoma cells in vitro need to be clarified.Methods: The A2058 and A375 melanoma cancer cells were treated with Terminalia catappa leaf extracts, and then the effect of Terminalia catappa leaf extracts on migration and invasion was examined. The cell migration/invasion capacities of A2058 and A375 cells were investigated by a modified Boyden chamber assay. Zymography was used to clarify the activities of matrix metalloproteinases-2 and urinary type plasminogen activator. We performed a Western blot to verify the related expression of phospho-Src (Tyr416), phospho-Focal adhesion kinase (Tyr397), Vimentin, and β-catenin.Results: Modified Boyden chamber assays demonstrated that treatment of Terminalia catappa leaf extracts significantly inhibited A2058 and A375 cell migration/invasion capacities. In the zymography results, we showed that Terminalia catappa leaf extracts negatively modulated the activities of matrix metalloproteinases-2 and urinary type plasminogen activator. Western blot indicated that Terminalia catappa leaf extracts reduced the expression of phospho-Src (Tyr416), phospho-Focal adhesion kinase (Tyr397), Vimentin, and β-catenin.Conclusion:Terminalia catappa leaf extracts affected the antimetastasis of the A2058 and A375 melanoma cell lines by inhibiting the Focal adhesion kinase/Src interaction and Wingless-int1/β-catenin pathways in vitro. Terminalia catappa leaf extracts may serve as an effective chemopreventive agent against metastasis of melanoma cancer.

Effectiveness of Ketapang (Terminalia cattapa L.) Extract Against Avian Pathogenic Eschericia coli (APEC) Infections in Layer Performance

“…As for saponins, its mechanism damages the bacteria's cell membranes, releasing important components, such as proteins, nucleic acids, and nucleotides; thus, bacteria turn into lysed. [12][13][14][15][16] Previous studies have discussed the antibacterial activity of Ketapang against various bacteria, 17 but none has discussed its antibacterial activity against APEC. This study aimed to determine the activity of Ketapang extract (Terminalia cattapa L.) in layers infected with APEC.…”

Evaluation of the Antitumor Activity of Quaternary Ammonium Surfactants

“…This type of cancer is associated with a variety of risk factors, including UV exposure and genetic conditions. Patients who develop metastases and have a prognosis of about 5 years account for less than 20% [2]. Undetected and untreated at an early stage, it quickly spreads to lymph nodes and nearby organs.…”

“…Surgical treatment, unfortunately, has no positive effect when metastasis is present. Therefore, there is a great need for new agents and therapeutic strategies [1][2][3]. Colorectal cancer accounts for 10.2% of all cancer cases and 9.2% of cancer deaths [4].…”

In Vitro Antioxidant and Antitrypanosomal Activities of Extract and Fractions of Terminalia catappa

“…In Brazil, it is found mainly in the Northeast region of the country [10]. Its leaves have biologically active compounds with some activities described in the literature, including anti-inflammatory and immunomodulatory [11], antidiabetic [12], antibacterial [13] and anticancer [14,15].…”