Terminalia paniculata bark extract attenuates non-alcoholic fatty liver via down regulation of fatty acid synthase in high fat diet-fed obese rats

Abstract

BackgroundThis study was performed to understand the possible therapeutic activity of Terminalia paniculata ethanolic extract (TPEE) on non alcoholic fatty liver in rats fed with high fat diet.MethodsThirty six SD rats were divided into 6 groups (n = 6): Normal control (NC), high fat diet (HFD), remaining four groups were fed on HFD along with different doses of TPEE (100,150 and 200 mg/kg b.wt) or orlistat, for ten weeks. Liver tissue was homogenized and analyzed for lipid profiles, activities of superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) content. Further, the expression levels of FAS and AMPK-1α were also studied in addition to histopathology examination of liver tissue in all the groups.ResultsHFD significantly increased hepatic liver total cholesterol (TC), triglycerides (TG), free fatty acids (FFA) and MDA but decreased the activities of SOD and CAT which were subsequently reversed by supplementation with TPEE in a dose-dependent manner. In addition, TPEE administration significantly down regulated hepatic mRNA expression of FAS but up regulated AMPK-1α compared to HFD alone fed group. Furthermore, western blot analysis of FAS has clearly demonstrated decreased expression of FAS in HFD + TPEE (200 mg/kg b.wt) treated group when compared to HFD group at protein level.ConclusionsOur biochemical studies on hepatic lipid profiles and antioxidant enzyme activities supported by histological and expression studies suggest a potential therapeutic role for TPEE in regulating obesity through FAS.

Evaluation of anti-obesity activities of ethanolic extract of Terminalia paniculata bark on high fat diet-induced obese rats

“…(Combretaceae) is a tropical tree with a large natural distribution in western and southern parts of India. Traditionally, its flower juice is used to treat cholera, inflammation of parotid glands and menstrual disorders, cough, bronchitis, cardiac debility, hepatitis, diabetes and obesity [ 6 ]. The bark is reported to contain 14 % tannins which have a pyrogallol nucleus along with gallic acid.…”

Antiobesity Activity of <i>Bauhinia purpurea</i> Extract: Effect on Hormones and Lipid Profile in High Calorie Diet Induced Obese Rats

“…The temperature of the heated transfer capillary was 325˚C. The mass spectrometer was scanned from m/z 100 to 1200 in full scan mode, gas flow 10 L/min; Nebulizer 40 psi [2].…”

“…The prevalence of obesity is escalating across the world cutting across age groups, sex and ethnicity. Obesity is one of the major contributors to morbid conditions like type-2 diabetes, hypertension, cardiovascular disorders, and non-alcoholic fatty liver metabolic syndrome [2] [3]. Though the etiology of obesity is diverse, the root cause is energy disparity: more calories consumed than expended.…”

In- vitroantioxidant and anti-obesity properties of Bauhinia variegata

“…Obesity is one of the major risk factor for hypertension, cardiovascular diseases, type 2 diabetes and non alcoholic fatty liver metabolic syndrome 5,6 . Identifying the exact cause of obesity is difficult since many factors influence energy balance and interact at different levels.…”

“…Various parts of the plants are used in indigenous medicine for the treatment of many ailments obesity, dyspepsia, bronchitis, leprosy, ulcer, and as an astringent, tonic and anthelmintic [8][9][10][11] . Phytochemical analysis of the leaves of Bauhinia variegata Linn was reported to contain, alkaloids, oil, fat glycosides, phenolics, lignins, saponins, terpinoids, β -sitosterol, tannins, kaempferol-3-glucoside, rutin, quercetin, quercetin, apigenin, apigenin-7-O-glucoside, lupeol amides, carbohydrates, reducing sugars, protein, vitamin C, fibers, calcium and phosphorus 5 . The beneficial therapeutic properties of the plants are mainly due to the presence of these components.…”