Terrestrial Microalgae: Novel Concepts for Biotechnology and Applications

Lakatos, Michael; Strieth, Dorina

doi:10.1007/124_2017_10

Cited by 16 publications

(23 citation statements)

References 191 publications

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“…For this reason, the temperature distribution on borosilicate glass, PMMA and silicone was investigated and simulated (see Figure 3). The temperatures of the surfaces in the cultivation area range were between 21.8-24.8 • C (borosilicate glass), 20.8-21.8 • C (PMMA) and 21-21.4 • C (silicone) and are within the temperature range tolerated by different cyanobacterial strains [21]. The simulated temperature profiles of the individual substrates show slight deviations compared to those determined experimentally.…”

Section: Temperature and Light Distribution In Epbrmentioning

confidence: 65%

“…In summary it can be said that the light intensities in the cultivation area (5-8 cm distance from the light source) are lower than the light compensation point of Nostoc sp. (formerly listed incorrectly as Trichocoleus sociatus) 90 ± 30 µmol photons m −2 s −1 [21] ) without taking scattered light into account. However, if the scattered light of 30 µmol photons m −2 s −1 is added to the transmitted light intensities, the light intensities are in the cultivation range for the substrates PMMA (49-115 µmol photons m −2 s −1 ) and borosilicate glass (39-93 µmol photons m −2 s −1 ) and in the area of the light compensation point.…”

Section: Temperature and Light Distribution In Epbrmentioning

confidence: 99%

“…The light compensation point of Nostoc sp. is 90 ± 65 µmol photons m −2 s −1 [21]. If stray light is included, the substrates consisting of PMMA and borosilicate glass cover this area.…”

Section: Influence Of Different Substrates On Biofilm Formationmentioning

confidence: 99%

“…For example, basic cations (Mg 2+ , Ca 2+ ) are available at low proton concentrations and mainly cationic acids (iron, cadmium, aluminium) are available at high proton concentrations. The development of biofilm PBRs face major challenges, but the imitation of natural habitats increases productivity of the cells [21][22][23]. To imitate conditions in the desert or the rain forest an aerosol-based PBR was developed by Kuhne et al [24].…”

Section: Introductionmentioning

confidence: 99%

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Characterization of an Aerosol-Based Photobioreactor for Cultivation of Phototrophic Biofilms

Strieth

Weber²,

Robert

et al. 2021

Life

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Phototrophic biofilms, in particular terrestrial cyanobacteria, offer a variety of biotechnologically interesting products such as natural dyes, antibiotics or dietary supplements. However, phototrophic biofilms are difficult to cultivate in submerged bioreactors. A new generation of biofilm photobioreactors imitates the natural habitat resulting in higher productivity. In this work, an aerosol-based photobioreactor is presented that was characterized for the cultivation of phototrophic biofilms. Experiments and simulation of aerosol distribution showed a uniform aerosol supply to biofilms. Compared to previous prototypes, the growth of the terrestrial cyanobacterium Nostoc sp. could be almost tripled. Different surfaces for biofilm growth were investigated regarding hydrophobicity, contact angle, light- and temperature distribution. Further, the results were successfully simulated. Finally, the growth of Nostoc sp. was investigated on different surfaces and the biofilm thickness was measured noninvasively using optical coherence tomography. It could be shown that the cultivation surface had no influence on biomass production, but did affect biofilm thickness.

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Section: Temperature and Light Distribution In Epbrmentioning

confidence: 65%

Section: Temperature and Light Distribution In Epbrmentioning

confidence: 99%

Section: Influence Of Different Substrates On Biofilm Formationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Characterization of an Aerosol-Based Photobioreactor for Cultivation of Phototrophic Biofilms

Strieth

Weber²,

Robert

et al. 2021

Life

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“…For terrestrial cyanobacteria, originated from emersed air-exposed habitats, however, the cultivation conditions in submerged fermentations are not optimal and their productivity is limited [15,16]. To address these limitations, a special biofilm cultivation system, the emersed photobioreactor (ePBR), was developed by Kuhne et al [17] and steadily refined over the past few years [18][19][20]. The ePBR imitates the natural habitat of terrestrial cyanobacteria by an air exposed cultivation and nutrient supply through an aerosol.…”

Section: Introductionmentioning

confidence: 99%

Influence of wettability and surface design on the adhesion of terrestrial cyanobacteria to additive manufactured biocarriers

Scherer

Soerjawinata

Schaefer

et al. 2022

Bioprocess Biosyst Eng

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Productive biofilms are gaining growing interest in research due to their potential of producing valuable compounds and bioactive substances such as antibiotics. This is supported by recent developments in biofilm photobioreactors that established the controlled phototrophic cultivation of algae and cyanobacteria. Cultivation of biofilms can be challenging due to the need of surfaces for biofilm adhesion. The total production of biomass, and thus production of e.g. bioactive substances, within the bioreactor volume highly depends on the available cultivation surface. To achieve an enlargement of surface area for biofilm photobioreactors, biocarriers can be implemented in the cultivation. Thereby, material properties and design of the biocarriers are important for initial biofilm formation and growth of cyanobacteria. In this study, special biocarriers were designed and additively manufactured to investigate different polymeric materials and surface designs regarding biofilm adhesion of the terrestrial cyanobacterium Nostoc flagelliforme (CCAP 1453/33). Properties of 3D-printed materials were characterized by determination of wettability, surface roughness, and density. To evaluate the influence of wettability on biofilm formation, material properties were specifically modified by gas-phase fluorination and biofilm formation was analyzed on biocarriers with basic and optimized geometry in shaking flask cultivation. We found that different polymeric materials revealed no significant differences in wettability and with identical surface design no significant effect on biomass adhesion was observed. However, materials treated with fluorination as well as optimized biocarrier design showed improved wettability and an increase in biomass adhesion per biocarrier surface.

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Passively immobilized cyanobacteria Nostoc species BB 92.2 in a moving bed photobioreactor (MBPBR): Design, cultivation, and characterization

Walther

Erdmann

Stoffel

et al. 2022

Biotech & Bioengineering

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The cyanobacterium Nostoc sp. BB 92.3. had shown antibacterial activity. A cultivation as biofilm, a self-forming matrix of cells and extracellular polymeric substances, increased the antibacterial effect. A new photobioreactor system was developed that allows a surface-associated cultivation of Nostoc sp. as biofilm. Highdensity polyethylene carriers operated as a moving bed were selected as surface for biomass immobilization. This system, well established in heterotrophic wastewater treatment, was for the first time used for phototrophic biofilms. The aim was a cultivation on a large scale without inhibiting growth while maximizing immobilization. Cultivation in a small photobioreactor (1.5 L) with different volumetric filling degrees of carriers (13.4%-53.8%) in a batch process achieved immobilization rates of 70%-85% and growth was similar to a no-carrier-control. In a larger photobioreactor (65 L) essentially all of the biomass was immobilized on the carriers and the space-time yield of biomass (0.018 g cell dry weight L −1 day −1 ) was competitive compared to phototrophic biofilm cultivations from literature. The use of carriers increased the gas exchange in the reactor by a factor of 2.5-3 but doubled the mixing time. Enriched gassing with carbon dioxide resulted in a shortterm increase in growth rate, but unexpectedly it also adversely changed the growth morphology.

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Terrestrial Microalgae: Novel Concepts for Biotechnology and Applications

Cited by 16 publications

References 191 publications

Characterization of an Aerosol-Based Photobioreactor for Cultivation of Phototrophic Biofilms

Characterization of an Aerosol-Based Photobioreactor for Cultivation of Phototrophic Biofilms

Influence of wettability and surface design on the adhesion of terrestrial cyanobacteria to additive manufactured biocarriers

Passively immobilized cyanobacteria Nostoc species BB 92.2 in a moving bed photobioreactor (MBPBR): Design, cultivation, and characterization

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