Tertiary Lymphoid Structures Are Related to Inflammatory Progression and Bone Loss in Human Apical Periodontitis

Liu, Jiayi; Yue, Junli; Wang, Konghuai; Zhang, Lu; Mao, Han-Qing; Chen, Zhi; Li, Qiuhui; Zhang, Lu

doi:10.1016/j.joen.2023.06.006

Cited by 5 publications

(1 citation statement)

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“…8c ). Few papers have described oral tertiary lymphoid structures as a common feature in periodontitis 48 , 49 . We quantified this by the same ROIs using single protein markers and manual thresholding to determine the positivity of by converting 0-255 auxiliary units of fluorescence to a binary classification of either negative (“0”) or positive (“1”; see: Methods).…”

Section: Resultsmentioning

confidence: 99%

Single-cell and spatially resolved interactomics of tooth-associated keratinocytes in periodontitis

Easter,

Fernandes Matuck,

Beldorati Stark

et al. 2024

Nat Commun

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Periodontitis affects billions of people worldwide. To address relationships of periodontal niche cell types and microbes in periodontitis, we generated an integrated single-cell RNA sequencing (scRNAseq) atlas of human periodontium (34-sample, 105918-cell), including sulcular and junctional keratinocytes (SK/JKs). SK/JKs displayed altered differentiation states and were enriched for effector cytokines in periodontitis. Single-cell metagenomics revealed 37 bacterial species with cell-specific tropism. Fluorescence in situ hybridization detected intracellular 16 S and mRNA signals of multiple species and correlated with SK/JK proinflammatory phenotypes in situ. Cell-cell communication analysis predicted keratinocyte-specific innate and adaptive immune interactions. Highly multiplexed immunofluorescence (33-antibody) revealed peri-epithelial immune foci, with innate cells often spatially constrained around JKs. Spatial phenotyping revealed immunosuppressed JK-microniches and SK-localized tertiary lymphoid structures in periodontitis. Here, we demonstrate impacts on and predicted interactomics of SK and JK cells in health and periodontitis, which requires further investigation to support precision periodontal interventions in states of chronic inflammation.

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Section: Resultsmentioning

confidence: 99%