Testes transcriptome profiles of the anadromous fish Coilia nasus during the onset of spermatogenesis

Zhou, Yanfeng; Duan, Jinrong; Li, Kai; Xu, Donghui; Zhang, Minying; Fang, Di‐An; Xu, Pao

doi:10.1016/j.margen.2015.06.007

Cited by 12 publications

(10 citation statements)

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“…During the fish spawning migration, factors which regulate the development of the germ cells are still poorly known [ 11 ]. Our results on the expression of HSP60 and HSP10 expand and complement earlier reports of germ-cell-specific gene expression [ 9 ]. Even though HSP60/HSP10 matrix activity has been recognized for years, its contribution to germ cell development is still unresolved [ 18 ].…”

Section: Discussionsupporting

confidence: 91%

“…In the C. nasus spermatids, as the IM results showed, condensed mitochondria with a diffuse and vacuolated matrix are found ( Figure 6 ), which is similar to the matrix-rich mitochondria of rat fetal and postnatal spermatogonia [ 14 ]. This makes biological sense in the process of protein assembly and import into the mitochondria, and therefore these proliferating spermatogenic cells would require more HSP60 to accommodate the generation of new mitochondria for daughter germ cells [ 9 , 27 , 32 ]. During spermatogenesis, mitochondria of spermatogenic cells must undergo rapid morphological changes.…”

Section: Discussionmentioning

confidence: 99%

“…Using a drift net, live healthy fish were sampled. Six populations of C. nasus were collected from six reaches in the Yangtze River during the anadromous period (from March to June, 2016), as described by Zhou and Fang [ 9 , 39 ]. All fish were field dissected to identify the developmental period and then we collected fishes which we needed.…”

Section: Methodsmentioning

confidence: 99%

“…Mature fish usually migrate upstream and spawn in the lower and middle reach of the Yangtze River in China, and then eggs float down and hatch near the river mouth [ 6 ]. During the fish spawning migration process, long distance swimming induced fish to consume more energy and encounter a lot of stress, such as water temperature and water flow, which results in up- or downregulation of numerous adaptive genes, including heat shock proteins (HSPs), to adapt to the migration behavior [ 7 , 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

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Developmental Expression of HSP60 and HSP10 in the Coilia nasus Testis during Upstream Spawning Migration

Fang

Zhou

Zhang

et al. 2017

Genes

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Heat shock protein 60 (HSP60) and heat shock protein 10 (HSP10) are important chaperones, which have been proven to have essential roles in mediating the correct folding of nuclear encoded proteins imported to mitochondria. Mitochondria are known as the power house of the cell, with which it produces energy and respires aerobically. In this regard, the obtained HSP60 and HSP10 have typical characteristics of the HSP60/10 family signature. Their mRNA transcripts detected were highest during the developmental phase (in April), while the lowest levels were found in the resting phase (after spawning in late July). Additionally, the strongest immunolabeling positive signals were found in the primary spermatocyte, with lower positive staining in secondary sperm cells, and a weak or absent level in the mature sperm. At the electron microscopic level, immunogold particles were localized in the mitochondrial matrix. Data indicated that HSP10 and HSP60 were inducible and functional in the Coilia nasus testis development and migration process, suggesting their essential roles in this process. The results also indicated that HSP60 may be one indicator of properly working mitochondrial import and refolding in the fish testis. This study also provides an expanded perspective on the role of heat shock protein families in spawning migration biology.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Developmental Expression of HSP60 and HSP10 in the Coilia nasus Testis during Upstream Spawning Migration

Fang

Zhou

Zhang

et al. 2017

Genes

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“…As a result, in recent years, more testis analyses have been performed using RNA-seq. For example, testis transcriptomes have been profiled by RNA-seq approaches in fish such as zebrafish [16], Nile tilapia [17], catfish [18], Southern bluefin tuna [19], guppy [20], Chinese sturgeon [21], sharpsnout seabream [22], Coilia nasus [23], and olive flounder [24]. In this way, RNA-seq has provided new insights into the molecular mechanisms underlying testis development and spermatogenesis.…”

Section: Introductionmentioning

confidence: 99%

Different expression patterns of sperm motility-related genes in testis of diploid and tetraploid cyprinid fish†

Zhou

et al. 2017

Biology of Reproduction

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Sperm motility is an important standard to measure the fertility of male. In our previous study, we found that the diploid spermatozoa from allotetraploid hybrid (4nAT) had longer durations of rapid and slow progressive motility than haploid spermatozoa from common carp (COC). In this study, to explore sperm motility-related molecular mechanisms, we compared the testis tissues transcriptomes from 2-year-old male COC and 4nAT. The RNA-seq data revealed that 2985 genes were differentially expressed between COC and 4nAT, including 2216 upregulated and 769 downregulated genes in 4nAT. Some differentially expressed genes, such as tubulin genes, dynein, axonemal, heavy chain(dnah) genes, mitogen-activated protein kinase(mapk) genes, tektin 4, FOX transcription factors, proteasome genes, and ubiquitin carboxyl-terminal hydrolase(uchl) genes, are involved in the regulation of cell division, flagellar and ciliary motility, gene transcription, cytoskeleton, energy metabolism, and the ubiquitin–proteasome system, suggesting that these genes were related to sperm motility of the 4nAT. We confirmed the differential expression of 12 such genes in 4nAT by quantitative PCR. By western blotting, we also confirmed increased expression of Uchl3 in 4nAT testis. In addition, we identified 1915 and 2551 predicted long noncoding RNA (lncRNA) transcripts from testis tissue transcriptomes of COC and 4nAT, respectively. Of these, 1575 lncRNAs were specifically expressed in 4nAT and 939 were specifically expressed in COC. This study provides insights into the transcriptome profile of testis tissues from diploid and tetraploid, which are useful for research on regulatory mechanisms behind sperm motility in male polyploidy.

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Identification of olfactory receptor genes in the Japanese grenadier anchovy Coilia nasus

et al. 2017

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Olfaction is essential for fish to detect odorant elements in the environment and plays a critical role in navigating, locating food and detecting predators. Olfactory function is produced by the olfactory transduction pathway and is activated by olfactory receptors (ORs) through the binding of odorant elements. Recently, four types of olfactory receptors have been identified in vertebrate olfactory epithelium, including main odorant receptors (MORs), vomeronasal type receptors (VRs), trace-amine associated receptors (TAARs) and formyl peptide receptors (FPRs). It has been hypothesized that migratory fish, which have the ability to perform spawning migration, use olfactory cues to return to natal rivers. Therefore, obtaining OR genes from migratory fish will provide a resource for the study of molecular mechanisms that underlie fish spawning migration behaviors. Previous studies of OR genes have mainly focused on genomic data, however little information has been gained at the transcript level. In this study, we identified the OR genes of an economically important commercial fish Coilia nasus through searching for olfactory epithelium transcriptomes. A total of 142 candidate MOR, 52 V2R/OlfC, 32 TAAR and two FPR putative genes were identified. In addition, through genomic analysis we identified several MOR genes containing introns, which is unusual for vertebrate MOR genes. The transcriptome-scale mining strategy proved to be fruitful in identifying large sets of OR genes from species whose genome information is unavailable. Our findings lay the foundation for further research into the possible molecular mechanisms underlying the spawning migration behavior in C. nasus.Electronic supplementary materialThe online version of this article (doi:10.1007/s13258-017-0517-8) contains supplementary material, which is available to authorized users.

show abstract

Testes transcriptome profiles of the anadromous fish Coilia nasus during the onset of spermatogenesis

Cited by 12 publications

References 14 publications

Developmental Expression of HSP60 and HSP10 in the Coilia nasus Testis during Upstream Spawning Migration

Developmental Expression of HSP60 and HSP10 in the Coilia nasus Testis during Upstream Spawning Migration

Different expression patterns of sperm motility-related genes in testis of diploid and tetraploid cyprinid fish†

Identification of olfactory receptor genes in the Japanese grenadier anchovy Coilia nasus

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