Testing and Evaluation of Bioactive Compounds in Soybean

Abstract

This study was aimed to estimate the antioxidants, antibacterial, and antifungal activities of methanolic extract from soybean seeds against pathogenic microorganisms. The methanolic extract was prepared from defatted soybean flour. Total phenolics content (TPC), total flavonoid content (TFC), and the major phenolic, flavonoid, and isoflavone compounds in soybean extract were estimated by HPLC. On the other hand, biological activities such as antioxidants, antibacterial, and antifungal activities were evaluated. The value of phenolic compounds was 8.59 mg GAE/g extract, while the value of flavonoids was 0.82 mg QE/g extract. The chemicals listed below were chromatographed and identified: syringic acid, quercetin, gallic acid, benzoic acid, genistein, daidzein, p-coumaric acid, glycitein, and ferulic acid. The methanolic extract showed the antibacterial and antifungal activity against tested microorganisms. Considering the results, it is possible to employ the methanolic extract from soybean seeds, which is rich in phenolic chemicals, as an antioxidant, antibacterial, and antifungal agent. It functions well as a pure, natural product.

Antimicrobial Activities of Natural Bioactive Polyphenols

“…C. albicans and T. rubrum are affected by daidzein and genistein. Glycitein shows effective antifungal as well as antibacterial properties [ 89 , 90 ]. In experiments using antimicrobial agents, strains susceptible to resveratrol (50 µg/mL) and curcumin (20 µg/mL) show sub-MIC levels lowering evaluated virulence factors by at least 50% (IC 50 ).…”

The Synergistic Effect of Germinating Soybean Seed in Prolonging the Shelf Life of Cold-Preserved Camel Meat

“…Surface conditioning is vital for cell growth, and often creates a favorable environment for bacterial growth, and enhances adhesion. Cells attach to surfaces, leading to infection [29]. Therefore, it can be assumed that the presence of the plant extract in the growth medium led to an unfavorable condition that could prevent cell attachment or reduce surface adhesion.…”

“…Many plant secondary metabolite compounds have characterized antibacterial activities against plant pathogens, which can be effectively applied to fight human pathogens. Moreover, the antibacterial activities of many metabolites are used differently from conventional drugs and can therefore be important in enhancing antibacterial therapy [3,29] also found that flavonoids should be found as effective antimicrobial agents against a wide range of pathogenic microorganisms in vitro.…”