Testing Motility Parameters of Post-Thawing Pesisir Bulls (Bos indicus) Semen with and without Sexing

Abstract

This study aimed to test the motility parameters of frozen semen from Pesisir bulls with and without sexing. The research material consisted of semen from two Pesisir bulls. The data obtained were statistically analyzed using ANOVA. The results of this study showed that the motility percentage of Pesisir bulls ranged from 53.66 to 58.87%, and progressive motility ranged from 41.20 to 48.87%. The values of distance curve line (DCL) ranged from 24.05 to 28.24μm, distance average path (DAP) ranged from 24.05 to 24.35μm, and distance straight-line (DSL) ranged from 18.49 to 22.78μm. The values of velocity curve line (VCL) ranged from 93.95 to 112.97μm/s, velocity average path (VAP) ranged from 53.90 to 62.57μm/s, and velocity straight-line (VSL) ranged from 41.97 to 50.51μm/s. The values of linearity (LIN) ranged from 0.43 to 0.45, straightness (STR) ranged from 0.75 to 0.80, and wobble (WOB) ranged from 0.54 to 0.59. Meanwhile, the amplitude of lateral head (ALH) value was between 4.22 and 5.14μm, and the beat cross frequency (BCF) value ranged from 21.34 to 26.99Hz. The motility parameters, progressive motility, DAP, DSL, VAP, VSL, and LIN of post-thawing Pesisir bulls semen did not show significant differences (P>0.05) both with and without sexing. However, there were significant differences (P<0.05) in the parameters of progressive motility, DCL, VCL, STR, WOB, ALH, and BCF. However, for future research, it is expected that studies could involve more significant and more diverse sample sizes, as well as variations in cryopreservation techniques that may be employed. Additionally, further research could consider factors such as the age and health condition of Pesisir bulls, which could influence semen quality after thawing.