Testing the protective effects of cyclodextrins vs. alternariol-induced acute toxicity in HeLa cells and in zebrafish embryos

Fliszár-Nyúl, Eszter; Bock, Illés; Csepregi, Rita; Szente, Lajos; Szabó, István; Csenki, Zsolt; Poór, Miklós

doi:10.1016/j.etap.2022.103965

Cited by 11 publications

(11 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Based on previous studies, alternariol and some of its derivatives form relatively stable complexes with SBECD and SGD [ 16 , 18 ]. Therefore, we selected these CDs to test their complex formation with AG, AMG, and Z14Glr.…”

Section: Resultsmentioning

confidence: 99%

“…CDs can accommodate lipophilic ligand molecules in their apolar cavity, forming host–guest-type inclusion complexes [ 17 ]. As has been recently reported, alternariol and its methyl and sulfate metabolites form relatively stable complexes with sulfobutylether-β-CD (SBECD) and with sugammadex (SGD; a chemically modified γ-CD) [ 16 , 18 ]. Furthermore, SGD bound alternariol and its methyl derivative with unexpectedly high affinity [ 16 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

“…As has been recently reported, alternariol and its methyl and sulfate metabolites form relatively stable complexes with sulfobutylether-β-CD (SBECD) and with sugammadex (SGD; a chemically modified γ-CD) [ 16 , 18 ]. Furthermore, SGD bound alternariol and its methyl derivative with unexpectedly high affinity [ 16 , 18 ]. Alternariol and its methyl/sulfate metabolites were successfully extracted from aqueous solutions with insoluble (but water-swellable) β-CD bead polymer (BBP) [ 16 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

“…Zearalenone, zearalenols, zearalanone, zearalanols, and zearalenone sulfate were almost completely removed from aqueous solutions by BBP [ 20 , 21 , 22 ]. In addition, certain CDs strongly alleviated or even ceased the alternariol- and zearalenone-induced toxic effects in cell experiments and in zebrafish studies [ 18 , 23 ]. These observations demonstrate that CDs are promising binders of these mycotoxins.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Probing Serum Albumins and Cyclodextrins as Binders of the Mycotoxin Metabolites Alternariol-3-Glucoside, Alternariol-9-Monomethylether-3-Glucoside, and Zearalenone-14-Glucuronide

et al. 2023

Self Cite

View full text Add to dashboard Cite

Mycotoxins are toxic metabolites of molds. Chronic exposure to alternariol, zearalenone, and their metabolites may cause the development of endocrine-disrupting and carcinogenic effects. Alternariol-3-glucoside (AG) and alternariol-9-monomethylether-3-glucoside (AMG) are masked derivatives of alternariol. Furthermore, in mammals, zearalenone-14-glucuronide (Z14Glr) is one of the most dominant metabolites of zearalenone. In this study, we examined serum albumins and cyclodextrins (CDs) as potential binders of AG, AMG, and Z14Glr. The most important results/conclusions were as follows: AG and AMG formed moderately strong complexes with human, bovine, porcine, and rat albumins. Rat albumin bound Z14Glr approximately 4.5-fold stronger than human albumin. AG–albumin and Z14Glr–albumin interactions were barely influenced by the environmental pH, while the formation of AMG–albumin complexes was strongly favored by alkaline conditions. Among the mycotoxin–CD complexes examined, AMG–sugammadex interaction proved to be the most stable. CD bead polymers decreased the mycotoxin content of aqueous solutions, with moderate removal of AG and AMG, while weak extraction of Z14Glr was observed. In conclusion, rat albumin is a relatively strong binder of Z14Glr, and albumin can form highly stable complexes with AMG at pH 8.5. Therefore, albumins can be considered as affinity proteins with regard to the latter mycotoxin metabolites.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Probing Serum Albumins and Cyclodextrins as Binders of the Mycotoxin Metabolites Alternariol-3-Glucoside, Alternariol-9-Monomethylether-3-Glucoside, and Zearalenone-14-Glucuronide

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, chronic exposure to CPA can cause degenerative effects (nephrotoxic, neurotoxic, etc. ), and its occurrence in food should not be underestimated [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

The Vapor Phase of Selected Essential Oils and Their Antifungal Activity In Vitro and In Situ against Penicillium commune, a Common Contaminant of Cheese

Hlebová

Foltínová

Vešelényiová

et al. 2022

Foods

View full text Add to dashboard Cite

This study aimed to determine the in vitro and in situ antifungal activity of (14) selected essential oils (EOS), namely clove, thyme, red thyme, litsea, eucalyptus, niaouli, fennel, anise, cumin, basil, rosemary, sage, bergamot mint, and marjoram, by vapor contact against the growth of two strains of Penicillium commune (KMi–183 and KMi–402). Furthermore, to exclude the negative effect of EOs on the lactic acid bacteria (LABs) (Streptococcus spp.) on cheeses, their influence was monitored. Next, the sensory evaluation of cheese treated by EOs was evaluated. The results show that litsea and clove EOs were the most effective in the vapor phase against both tested strains. These EOs were characterized by the highest amount of α- (40.00%) and β-Citral (34.35%) in litsea and eugenol (85.23%) in clove. The antitoxicogenic activity of less effective (in growth inhibition) EOs on cyclopiazonic acid (CPA) production by the tested strains was also observed. The growth of Streptococcus spp. (ranging from 8.11 to 9.69 log CFU/g) was not affected by the EOs in treated cheese. Even though the evaluators recognized some EOs in sensory evaluation by the triangle test, they did not have a negative effect on the taste and smell of the treated cheeses and were evaluated as edible. The antifungal activity of EOs against several types of microscopic fungi and their effect on the sensory properties of treated foods needs to be further tested to achieve the most effective protection of foods from their direct contaminants.

show abstract

Photo‐Stimuli Smart Supramolecular Self‐Assembly of Azobenzene/β‐Cyclodextrin Inclusion Complex for Controlling Plant Bacterial Diseases

Yang

Xiang

et al. 2023

Adv Funct Materials

View full text Add to dashboard Cite

Controllable and on‐demand delivery of supramolecular systems have received considerable attention in modern agricultural management, especially for managing intractable plant diseases. Here, an intelligent photoresponsive pesticide delivery system is reported based on β‐cyclodextrin (β‐CD) and azobenzene, which overcomes the resistance of phytopathogens caused by the irrational use of conventional pesticides. Antibacterial bioassays illustrated that designed azobenzene derivative 3a possesses the most efficient bioactivity with EC50 values of 0.52–25.31 µg mL−1 toward three typical phytopathogens. Moreover, the assembly of the supramolecular binary complex 3a@β‐CD is successfully elucidated and displays exceptional inhibitory activity on biofilm formation. Of note, this supramolecular binary complex significantly improves the water solubility, foliar surface wettability, and shows marked light‐responsive properties. In vivo anti‐Xoo assays reveal that 3a@β‐CD has excellent control efficiency (protective activity: 51.22%, curative activity: 48.37%) against rice bacterial blight pathogens, and their control efficiency can be elevated to values of 55.84% (protective activity) and 52.05% (curative activity) by UV–vis exposure. In addition, the 3a@β‐CD are non‐toxic toward various non‐target organisms. This study therefore offers new insights into the potential of host‐guest complexes as a feasible pesticide discovery strategy characterized by a safe, biocompatible, light‐responsive release, and antibiofilm properties for overcoming intractable plant bacterial diseases.

show abstract

Testing the protective effects of cyclodextrins vs. alternariol-induced acute toxicity in HeLa cells and in zebrafish embryos

Cited by 11 publications

References 44 publications

Probing Serum Albumins and Cyclodextrins as Binders of the Mycotoxin Metabolites Alternariol-3-Glucoside, Alternariol-9-Monomethylether-3-Glucoside, and Zearalenone-14-Glucuronide

Probing Serum Albumins and Cyclodextrins as Binders of the Mycotoxin Metabolites Alternariol-3-Glucoside, Alternariol-9-Monomethylether-3-Glucoside, and Zearalenone-14-Glucuronide

The Vapor Phase of Selected Essential Oils and Their Antifungal Activity In Vitro and In Situ against Penicillium commune, a Common Contaminant of Cheese

Photo‐Stimuli Smart Supramolecular Self‐Assembly of Azobenzene/β‐Cyclodextrin Inclusion Complex for Controlling Plant Bacterial Diseases

Contact Info

Product

Resources

About