Testing the utility of matK and ITS DNA regions for discrimination of Allium species

Abstract

IntroductionAbout 750 species have been recognized within the genus Allium L. (Stearn, 1992) and at least 20 of these species are edible (Fritsch and Friesen, 2002). Economically imported and widely cultivated edible Allium species are onion (A. cepa L.), garlic (A. sativum L.), leek (A. porrum L.), chive (A. schoenoprasum L.), Chinese chive (A. tuberosum Rottl. ex Spreng), rakkyo (A. chinense G.Don) and Japanese bunching onion (A. fistulosum L.). Remaining edible Allium species are either cultivated locally or collected from wild for local consumption.The phylogeny of the genus Allium has been revised using several molecular approaches. The initial molecular approach for phylogenetic analyses of the genus Allium was based on restriction enzyme analysis of chloroplast DNA (Havey, 1991;von Berg et al., 1996). In these studies, the phylogenetic relationships among Allium species were found to be generally in agreement with their previous traditional taxonomic classification. Later nucleotide sequences of the some conserved DNA regions were utilized for the phylogenetic reconstruction of the genus Allium. The nucleotide sequence of the nuclear ribosomal internal transcribed spacer region (ITS) was one of the most commonly used DNA regions for discrimination of the

Phylogenetic relationships of Iranian Allium sect. Allium (Amaryllidaceae, Allioideae) as inferred from nrDNA ITS, cpDNA rps16 and trnL–F sequences

“…Allium were also included: Allium rubellum M.Bieb., Allium rhodopeum Velen., Allium longivaginatum Wendelbo. In order to recover most of the clades currently recognized in the phylogeny of the genus (according to Friesen et al 2006, Li et al 2010, İpek et al 2014 we also included sequences of 28 representative species from GenBank (< www.ncbi.nlm. nih.gov >).…”

“…1, 2. There have been several molecular phylogenetic studies in Allium (Dubouzet and Shinoda 1998, 1999, Friesen et al 2006, Gurushidze et al 2007, Nguyen et al 2008, Hirschegger et al 2010, Li et al 2010, Choi et al 2012, Wheeler et al 2013, İpek et al 2014, Herden et al 2016, that addressed the infrageneric classification and the relationships in certain species groups. Based on a large-scale molecular systematic study, Allium has been classified into three evolutionary lines, 15 subgenera and 72 sections including A. subg.…”

Molecular phylogeny of Astragalus section Anthylloidei (Fabaceae) inferred from nrDNA ITS and plastid rpl32-trnL(UAG) sequence data

“…The internal transcribed spacer (ITS) contains the signals needed to process the rRNA transcript (Baldwin et al, 1995) and has often been used for inferring phylogeny at intra-and intergeneric levels (Wojchiekhowski et al, 1999;Kazempour Osaloo et al, 2003, 2005Javanmardi et al, 2012;Taşci Margoz et al, 2013;İpek et al, 2014). The rpl32-trnL (UAG) region is located in the SSC region of the chloroplast genome.…”

Aksaray’da Hami̇di̇ye Mahallesi̇’ne Çerkes İskâni Ve Çerkesleri̇n Kültürel Özelli̇kleri̇

“…Bu hususta kaza ve nahiyedeki memurları sıkıştırıp birtakım taşkınlıklar yapmışlar ve herhangi bir işte çalışmayı reddetmişlerdir. 88 Çerkesler iskân edildikleri yörelerin insanları ile anlaşma noktasında uyum sıkıntısı yaşamışlardır. Bu durum birçok sosyal problemi de beraberinde getirmiştir.…”